FastQCFastQC Report
Mon 26 Jun 2023
SRR392995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6431605
Total Bases463 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT2444273.8004044091638094RNA PCR Primer, Index 31 (95% over 21bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT1776072.761472447390659TruSeq Adapter, Index 11 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT1235611.921153429042984RNA PCR Primer, Index 41 (95% over 23bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC920781.4316488652521415RNA PCR Primer, Index 14 (95% over 22bp)
GTTCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAA919541.4297208861551665RNA PCR Primer, Index 45 (100% over 27bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCATCTCGTATGCCGTCTTC703071.0931485997663102RNA PCR Primer, Index 20 (100% over 21bp)
TCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAA679211.0560505503680653RNA PCR Primer, Index 45 (100% over 27bp)
TTCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAA624080.9703332216452969RNA PCR Primer, Index 45 (96% over 30bp)
ACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGCT554170.8616356259440685RNA PCR Primer, Index 33 (100% over 25bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCATCTCGTATGCCGTCTTCT526430.8185048677585145TruSeq Adapter, Index 18 (100% over 24bp)
AGTTCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAA523190.813467244956741RNA PCR Primer, Index 45 (96% over 30bp)
TACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGC436150.678135550923914RNA PCR Primer, Index 21 (96% over 25bp)
CTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA421450.6552796696936457RNA PCR Primer, Index 45 (96% over 30bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC403300.6270596530726001RNA PCR Primer, Index 20 (95% over 24bp)
TCTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCT377330.5866809295657927RNA PCR Primer, Index 21 (95% over 23bp)
CTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTG355900.5533610972688777RNA PCR Primer, Index 21 (95% over 24bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG352080.5474216777927127Illumina PCR Primer Index 10 (96% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC338920.5269602222151392TruSeq Adapter, Index 18 (95% over 22bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT291660.45347934147075264RNA PCR Primer, Index 3 (95% over 21bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCATCTCGTATGCCGTCTTCTGCT286180.444958917719605RNA PCR Primer, Index 24 (96% over 27bp)
GTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA281150.43713816380203696Illumina PCR Primer Index 3 (96% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG279640.43479038280491417TruSeq Adapter, Index 3 (96% over 26bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC278290.43269137330417523TruSeq Adapter, Index 3 (95% over 22bp)
CGCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGC255660.39750575478438116TruSeq Adapter, Index 3 (96% over 26bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG242170.3765312079955159No Hit
CAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGCTT228800.3557432398289385RNA PCR Primer, Index 21 (96% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG217470.3381271082412555TruSeq Adapter, Index 18 (95% over 24bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCTGTATCTCGTATGCCGTC213450.3318767243946107No Hit
ACAGTCCGACGATCTTTCGCACTCTGGAATCTCGTATGCCGTCTTCTGCT210940.3279741215450887Illumina PCR Primer Index 8 (96% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGGCTT208150.32363616857689487No Hit
TTCTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTC202780.31528677522951115RNA PCR Primer, Index 33 (100% over 21bp)
GTTCAGAGTTCTACAGTCGACGATCTTTCATCTCGTATGCCGTCTTCTGC191980.2984946992235997RNA PCR Primer, Index 45 (96% over 27bp)
TACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA190740.2965667201266247RNA PCR Primer, Index 45 (100% over 27bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC190000.2954161519558493TruSeq Adapter, Index 3 (96% over 25bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA184710.2871911443566575TruSeq Adapter, Index 3 (96% over 29bp)
TACAGTCCGACGATCTTTCGCACTCTGGAATCTCGTATGCCGTCTTCTGC162310.2523631348629153Illumina PCR Primer Index 8 (96% over 26bp)
TCTACAGTCCGACGATCTTTCGCACTCTGGAATCTCGTATGCCGTCTTCT157750.24527314721597485Illumina PCR Primer Index 8 (95% over 24bp)
CTACAGTCCGACGATCTTTCGCACTCTGGAATCTCGTATGCCGTCTTCTG157110.24427806123043938Illumina PCR Primer Index 8 (96% over 25bp)
GTTCTACAGTCCGACGATCTTTCGATCTCGTATGCCGTCTTCTGCTTGAA156720.24367168070800366RNA PCR Primer, Index 21 (100% over 29bp)
GGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTG154520.2402510726327254TruSeq Adapter, Index 18 (100% over 28bp)
GATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA149380.2322592883113935TruSeq Adapter, Index 18 (100% over 27bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTATCTCGTATGCCGTCTTCTGC139720.21723970921721716RNA PCR Primer, Index 38 (100% over 26bp)
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA139510.21691319662821332RNA PCR Primer, Index 38 (100% over 27bp)
AGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTTCTGCTTG128150.19925042038495835RNA PCR Primer, Index 33 (100% over 27bp)
AGTTCTACAGTCCGACGATCTTTCGATCTCGTATGCCGTCTTCTGCTTGA127760.19864403986252266RNA PCR Primer, Index 21 (96% over 30bp)
GTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTT127120.19764895387698717RNA PCR Primer, Index 24 (96% over 28bp)
GTTCTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCTT123410.19188056480458612RNA PCR Primer, Index 21 (95% over 21bp)
GCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTGA122210.19001477858170704TruSeq Adapter, Index 18 (96% over 29bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT121980.18965716955565523TruSeq Adapter, Index 7 (95% over 23bp)
CGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTG120440.18726274390296047RNA PCR Primer, Index 34 (95% over 24bp)
TCTACAGTCCGACGATCTTTCGATCTCGTATGCCGTCTTCTGCTTGAAAA117620.1828781462791947RNA PCR Primer, Index 21 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC116720.18147880661203541RNA PCR Primer, Index 41 (95% over 22bp)
ACAGTCCGACGATCTTTCTACTCTGCGATCTCGTATGCCGTCTTCTGCTT114430.17791826457004123RNA PCR Primer, Index 21 (96% over 27bp)
CCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCT113920.17712530542531763TruSeq Adapter, Index 3 (95% over 23bp)
GGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGAA113350.17623905696945008RNA PCR Primer, Index 41 (96% over 28bp)
CGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCT111690.1736580526944674RNA PCR Primer, Index 34 (96% over 28bp)
TTCTACAGTCCGACGATCTTTCGATCTCGTATGCCGTCTTCTGCTTGAAA107450.16706560804029477RNA PCR Primer, Index 21 (96% over 30bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA105730.16439131445416813RNA PCR Primer, Index 31 (96% over 28bp)
GTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC101130.1572391339331318RNA PCR Primer, Index 39 (96% over 25bp)
CAGTCCGACGATCTTTCGCACTCTGGAATCTCGTATGCCGTCTTCTGCTT96100.1494183800155638Illumina PCR Primer Index 8 (96% over 28bp)
GTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGCTTGA95100.14786355816316457TruSeq Adapter, Index 3 (96% over 28bp)
TACAGTCCGACGATCTTTCTACTCTGCGATCTCGTATGCCGTCTTCTGCT91730.14262380852057924RNA PCR Primer, Index 33 (100% over 25bp)
CTACAGTCCGACGATCTTTCGATCTCGTATGCCGTCTTCTGCTTGAAAAA90450.14063363654950825RNA PCR Primer, Index 21 (96% over 30bp)
TTCTACAGTCCGACGATCTTTCGCACTCTGGAATCTCGTATGCCGTCTTC87870.1366221961703183Illumina PCR Primer Index 8 (95% over 23bp)
ACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA87440.13595362277378661RNA PCR Primer, Index 45 (96% over 30bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC86590.13463202419924733RNA PCR Primer, Index 31 (95% over 22bp)
GTTCAGAGTTCTACAGTCCGACGGATCTTTCATCTCGTATGCCGTCTTCT81920.12737100614854302Illumina DpnII expression Sequencing Primer (95% over 23bp)
GCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCT81880.12730881327444704RNA PCR Primer, Index 3 (96% over 27bp)
TCTACAGTCCGACGATCTTTCTACTCTGCGATCTCGTATGCCGTCTTCTG81650.12695120424839523RNA PCR Primer, Index 33 (100% over 23bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG79760.12401259094736072Illumina PCR Primer Index 3 (96% over 28bp)
AGTTCTACAGTCCGACGATCTTTCGTACTCTGCGATCTCGTATGCCGTCT79190.12312634249149318No Hit
CCCTGGGCGCGCGCCGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA78600.12220899759857766Illumina PCR Primer Index 1 (96% over 28bp)
GAGTTCTACAGTCCGACGATCTTTCATCTCGTATGCCGTCTTCTGCTTGA78520.12208461185038572RNA PCR Primer, Index 45 (96% over 30bp)
CTACAGTCCGACGATCTTTCTACTCTGCGATCTCGTATGCCGTCTTCTGC77790.1209495918981343RNA PCR Primer, Index 21 (96% over 25bp)
GAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAA76680.11922373964197118Illumina PCR Primer Index 3 (96% over 28bp)
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC76400.11878838952329938RNA PCR Primer, Index 38 (100% over 25bp)
GCTCAGTCGGTAGAGCATGAGAATCTCGTATGCCGTCTTCTGCTTGAAAA76240.11853961802691552RNA PCR Primer, Index 26 (96% over 31bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT76110.11833749118610362No Hit
AGTCCGACGATCTTTCGCACTCTGGAATCTCGTATGCCGTCTTCTGCTTG73030.11354863988071408Illumina PCR Primer Index 8 (96% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAATCTCGTATGCCGTCTTCT66770.10381545508469504RNA PCR Primer, Index 19 (95% over 24bp)
GTATAGTGGACAGTATCTCCGCATCTCGTATGCCGTCTTCTGCTTGAAAA66150.10285146553620753TruSeq Adapter, Index 18 (96% over 30bp)
TACAGTCCGACGATCTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA64710.10061252206875267TruSeq Adapter, Index 25 (96% over 29bp)
GCGTTGGTGGTGTAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC64520.10031710591679682RNA PCR Primer, Index 20 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)