FastQCFastQC Report
Mon 26 Jun 2023
SRR392996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616709
Total Bases116.4 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAA378372.340371705730592Illumina Paired End PCR Primer 2 (96% over 27bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC337812.0894916772282457RNA PCR Primer, Index 14 (95% over 22bp)
AGTTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAA261331.6164318996182987TruSeq Adapter, Index 18 (96% over 28bp)
TTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAA234091.4479414662750067RNA PCR Primer, Index 18 (96% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT204711.266214266141897TruSeq Adapter, Index 11 (95% over 24bp)
TCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAA198951.2305863331001434Illumina Paired End PCR Primer 2 (96% over 27bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAA154250.9540987277240368Illumina Paired End PCR Primer 2 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT123170.7618563390195762RNA PCR Primer, Index 41 (95% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT113440.7016723479611977RNA PCR Primer, Index 31 (95% over 21bp)
CTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA107550.6652403122639882TruSeq Adapter, Index 18 (96% over 28bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT76490.47312163166036686RNA PCR Primer, Index 20 (96% over 25bp)
CGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTCT72560.44881298984542056RNA PCR Primer, Index 20 (95% over 23bp)
TACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA68460.42345282917333915Illumina Paired End PCR Primer 2 (96% over 27bp)
ACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA67960.4203601266523536TruSeq Adapter, Index 18 (96% over 28bp)
ACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCTGCT66650.4122572460473715RNA PCR Primer, Index 33 (100% over 25bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC58180.3598668653418766RNA PCR Primer, Index 20 (95% over 24bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAC55420.34279514742603645Illumina Paired End PCR Primer 2 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG53050.32813573747656505TruSeq Adapter, Index 3 (96% over 26bp)
GTTCAGAGTTCTACAGTCGACGATCCCCCATCTCGTATGCCGTCTTCTGC47620.29454898809866215RNA PCR Primer, Index 18 (96% over 25bp)
AGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA38890.24055040208225473Illumina Paired End PCR Primer 2 (96% over 27bp)
TACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCTGC37960.23479797539322167RNA PCR Primer, Index 21 (96% over 25bp)
ACAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCTGCTT37890.23436499704028368RNA PCR Primer, Index 33 (100% over 26bp)
GTTCTACAGTCCGACGATCCCCATCTCGTATGCCGTCTTCTGCTTGAAAA37660.23294235388063037TruSeq Adapter, Index 18 (96% over 29bp)
CGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTG37240.23034448376300248TruSeq Adapter, Index 11 (96% over 25bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG33320.2060976959984759Illumina PCR Primer Index 10 (96% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC33300.2059739878976365RNA PCR Primer, Index 41 (95% over 22bp)
GCGTTGGTGGTATAGTGGTTAGCATAGCTGCCATCTCGTATGCCGTCTTC31090.19230424275488042RNA PCR Primer, Index 14 (95% over 22bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAACA30940.19137643199858478Illumina Paired End PCR Primer 2 (100% over 24bp)
TCTACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCT30910.19119086984732564RNA PCR Primer, Index 21 (95% over 23bp)
CTACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCTG29950.1852528810070334RNA PCR Primer, Index 21 (95% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC29640.1833354054440224RNA PCR Primer, Index 31 (95% over 22bp)
TGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA28780.17801595710792728Illumina PCR Primer Index 3 (96% over 28bp)
TACAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCTGCT25990.160758677040828RNA PCR Primer, Index 21 (96% over 26bp)
GTTCTACAGTCCGACGATCCCCCGATCTCGTATGCCGTCTTCTGCTTGAA25350.15680001781396652Illumina PCR Primer Index 1 (96% over 28bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAACC25330.1566763097131271Illumina Paired End PCR Primer 2 (100% over 24bp)
AGTTCTACAGTCCGACGATCCCCATCTCGTATGCCGTCTTCTGCTTGAAA25210.15593406110809058RNA PCR Primer, Index 18 (96% over 29bp)
AGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCTGCTTG24510.1516042775787108RNA PCR Primer, Index 33 (100% over 27bp)
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAA24490.15148056947787142Illumina Paired End PCR Primer 2 (100% over 23bp)
TCTACAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCTG23740.14684151569639312RNA PCR Primer, Index 21 (95% over 24bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCATCTCGTATGCCGTCTTC22900.1416457754611374RNA PCR Primer, Index 20 (100% over 21bp)
TTCTACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTC22600.13979015394854608RNA PCR Primer, Index 33 (100% over 21bp)
CTACAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCTGC21630.13379031105783415RNA PCR Primer, Index 33 (100% over 24bp)
TTCTACAGTCCGACGATCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAA21200.1311305868897866RNA PCR Primer, Index 18 (96% over 29bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAG20300.12556372235201263Illumina Paired End PCR Primer 2 (100% over 24bp)
AGTTCTACAGTCCGACGATCCCCCGATCTCGTATGCCGTCTTCTGCTTGA19940.12333697653690305Illumina Paired End PCR Primer 2 (96% over 28bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACAC19240.1190071930075233Illumina Paired End PCR Primer 2 (100% over 24bp)
GCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA18790.11622376073863631TruSeq Adapter, Index 18 (96% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCATCTCGTATGCCGTCTTCTGCT18080.11183212315883688RNA PCR Primer, Index 24 (96% over 27bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACAA17930.11090431240254123Illumina Paired End PCR Primer 2 (100% over 24bp)
CATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCTG17760.10985279354540614RNA PCR Primer, Index 20 (96% over 25bp)
TTCTACAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCT17550.1085538584865922RNA PCR Primer, Index 21 (95% over 23bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAACCC16810.10397665875553361Illumina Paired End PCR Primer 2 (100% over 24bp)
CAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA16710.1033581182513365TruSeq Adapter, Index 18 (96% over 28bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCATCTCGTATGCCGTCTTCT16190.10014170762951155TruSeq Adapter, Index 18 (100% over 24bp)
GTTCTACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTT16190.10014170762951155RNA PCR Primer, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)