FastQCFastQC Report
Mon 26 Jun 2023
SRR392997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8965169
Total Bases645.4 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC6388757.126190259213184RNA PCR Primer, Index 14 (95% over 22bp)
GTTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAA3979234.438544326381354Illumina Paired End PCR Primer 2 (96% over 27bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT3234813.6081974584082013TruSeq Adapter, Index 11 (95% over 24bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC2399042.675956248008264RNA PCR Primer, Index 20 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT2108362.3517236540660864RNA PCR Primer, Index 31 (95% over 21bp)
AGTTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAA2094142.335862268742508TruSeq Adapter, Index 18 (96% over 28bp)
TTCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAA2002812.233990234874546RNA PCR Primer, Index 18 (96% over 28bp)
TCTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAA1841452.0540047822857552Illumina Paired End PCR Primer 2 (96% over 27bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT1415431.5788101707842874RNA PCR Primer, Index 20 (96% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT976641.0893715444739525RNA PCR Primer, Index 41 (95% over 23bp)
CTACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA878830.9802715375471449TruSeq Adapter, Index 18 (96% over 28bp)
CGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTCT742140.8278036922672624RNA PCR Primer, Index 20 (95% over 23bp)
ACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCTGCT547660.6108752662665924RNA PCR Primer, Index 33 (100% over 25bp)
TACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA424030.47297490989851954Illumina Paired End PCR Primer 2 (96% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG402510.44897090060432765TruSeq Adapter, Index 3 (96% over 26bp)
TACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCTGC385860.43039902538368213RNA PCR Primer, Index 21 (96% over 25bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG373040.4160992391777556Illumina PCR Primer Index 10 (96% over 26bp)
TGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA367690.41013169969244306Illumina PCR Primer Index 3 (96% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC346870.3869084899570772RNA PCR Primer, Index 41 (95% over 22bp)
CTACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCTG312040.3480581347657808RNA PCR Primer, Index 21 (95% over 24bp)
CGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTG304810.33999359075104996TruSeq Adapter, Index 11 (96% over 25bp)
CGCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCT300710.33542033619221234Illumina PCR Primer Index 3 (96% over 26bp)
GTTCTACAGTCCGACGATCCCCATCTCGTATGCCGTCTTCTGCTTGAAAA295000.329051242648075TruSeq Adapter, Index 18 (96% over 29bp)
TCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTG289560.32298331464805624RNA PCR Primer, Index 41 (95% over 24bp)
ACAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCTGCTT282230.3148072278392075RNA PCR Primer, Index 33 (100% over 26bp)
TCTACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCT269550.3006636015450462RNA PCR Primer, Index 21 (95% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC268080.29902392247151166RNA PCR Primer, Index 31 (95% over 22bp)
TACAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCTGCT258820.28869505973618564RNA PCR Primer, Index 21 (96% over 26bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCATCTCGTATGCCGTCTTC255880.2854157015891167RNA PCR Primer, Index 20 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC250870.2798274076038053TruSeq Adapter, Index 18 (95% over 22bp)
AGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCTGCTTG238660.2662080324419986RNA PCR Primer, Index 33 (100% over 27bp)
ACAGTCCGACGATCCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA234370.2614228465743368TruSeq Adapter, Index 18 (96% over 28bp)
CTACAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCTGC227370.2536148509860774RNA PCR Primer, Index 33 (100% over 24bp)
GCGTTGGTGGTATAGTGGTTAGCATAGCTGCCATCTCGTATGCCGTCTTC215210.2400512472213296RNA PCR Primer, Index 14 (95% over 22bp)
CAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCTGCTT215050.2398727787507408RNA PCR Primer, Index 21 (96% over 27bp)
GCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCTT213710.23837810530955972Illumina PCR Primer Index 3 (96% over 27bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA200690.22385523351539718TruSeq Adapter, Index 3 (96% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGGCTT195520.21808847105949705No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCATCTCGTATGCCGTCTTCTGCT192790.21504335278007586RNA PCR Primer, Index 24 (96% over 27bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC192560.21478680435360448TruSeq Adapter, Index 3 (96% over 25bp)
ACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCTTGAAA190800.21282365117712784Illumina PCR Primer Index 3 (96% over 28bp)
GTTCTACAGTCCGACGATCCCCCGATCTCGTATGCCGTCTTCTGCTTGAA184160.2054172096476932Illumina PCR Primer Index 1 (96% over 28bp)
TCTACAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCTG182480.20354329070651095RNA PCR Primer, Index 21 (95% over 24bp)
GCGTTGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTCT177080.1975199798241394RNA PCR Primer, Index 14 (95% over 23bp)
AGTTCTACAGTCCGACGATCCCCATCTCGTATGCCGTCTTCTGCTTGAAA167300.18661109455939984RNA PCR Primer, Index 18 (96% over 29bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC161950.18064355507408728TruSeq Adapter, Index 3 (95% over 22bp)
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT159910.17836808207408025RNA PCR Primer, Index 41 (95% over 21bp)
TTCTACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTC149900.1672026483828693RNA PCR Primer, Index 33 (100% over 21bp)
AGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCTGCTTGA145670.16248438819167826RNA PCR Primer, Index 33 (100% over 27bp)
GATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA140910.15717495119166186TruSeq Adapter, Index 18 (100% over 27bp)
CAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCTGCTTG137500.15337134191223836RNA PCR Primer, Index 21 (96% over 28bp)
CGCGCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTG135970.15166473716223308Illumina PCR Primer Index 3 (95% over 24bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTATCTCGTATGCCGTCTTCTGC134390.14990236101516882RNA PCR Primer, Index 38 (100% over 26bp)
TTCTACAGTCCGACGATCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAA134390.14990236101516882RNA PCR Primer, Index 18 (96% over 29bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA128060.14284170214749994RNA PCR Primer, Index 31 (96% over 28bp)
CGCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGC127830.14258515372102856TruSeq Adapter, Index 3 (96% over 26bp)
GTCCTCGCCTGCCGCGCGATGCCTCCGCTGGCCATCTCGTATGCCGTCTT127600.14232860529455718RNA PCR Primer, Index 20 (95% over 23bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT126010.14055507486808114RNA PCR Primer, Index 3 (95% over 21bp)
AGTTCTACAGTCCGACGATCCCCCGATCTCGTATGCCGTCTTCTGCTTGA122000.13608220882394967Illumina Paired End PCR Primer 2 (96% over 28bp)
GCGTTGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTG119460.1332490218533527TruSeq Adapter, Index 18 (95% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC118750.13245706801511492RNA PCR Primer, Index 31 (95% over 22bp)
GTTCTACAGTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTT117840.1314420285886412RNA PCR Primer, Index 21 (95% over 21bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCATCTCGTATGCCGTCTTCT117180.13070584614746247TruSeq Adapter, Index 18 (100% over 24bp)
GGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCT114450.1276607278680413RNA PCR Primer, Index 24 (95% over 24bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG114170.12734840804451092No Hit
TCTACAGTCCGACGATCCCCCGATCTCGTATGCCGTCTTCTGCTTGAAAA113010.12605451163274223Illumina Paired End PCR Primer 2 (96% over 28bp)
TCTACAGTCCGACGATCCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA112130.1250729350445039TruSeq Adapter, Index 18 (96% over 29bp)
GCGTTGGTGGTGTAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC110760.12354479876508742RNA PCR Primer, Index 20 (95% over 22bp)
GGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA109760.12242937082390751Illumina PCR Primer Index 3 (96% over 28bp)
GCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA108940.12151471991213997TruSeq Adapter, Index 18 (96% over 28bp)
GCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTGA108680.12122470864743319TruSeq Adapter, Index 18 (96% over 29bp)
GTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTT108670.12121355436802139RNA PCR Primer, Index 24 (96% over 28bp)
GGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTG107220.11959618385331051TruSeq Adapter, Index 18 (100% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGGCTTC105450.11762187639742207RNA PCR Primer, Index 20 (95% over 21bp)
TTCTACAGTCCGACGATCCCCCTACTCTGCGATCTCGTATGCCGTCTTCT103620.11558064326506283RNA PCR Primer, Index 21 (95% over 23bp)
TACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCTTGAA103100.11500062073564928Illumina PCR Primer Index 3 (96% over 28bp)
GTTCTACAGTCCGACGATCCCCCCATCTCGTATGCCGTCTTCTGCTTGAA101680.1134167130591738TruSeq Adapter, Index 18 (96% over 28bp)
TTCTACAGTCCGACGATCCCCCGATCTCGTATGCCGTCTTCTGCTTGAAA99230.110683914603283Illumina PCR Primer Index 1 (96% over 28bp)
CGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTG98640.11002581211798684RNA PCR Primer, Index 34 (95% over 24bp)
CCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAA96320.10743801929444943Illumina PCR Primer Index 3 (96% over 28bp)
GCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTG95810.10686915104444769RNA PCR Primer, Index 34 (96% over 26bp)
CGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCT92110.10274206766208199RNA PCR Primer, Index 34 (96% over 28bp)
GTCCGACGATCCCCCGTACTCTGCGATCTCGTATGCCGTCTTCTGCTTGA90100.10050005750031038RNA PCR Primer, Index 21 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)