FastQCFastQC Report
Mon 26 Jun 2023
SRR392998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956621
Total Bases284.8 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT1295453.274132144574878RNA PCR Primer, Index 31 (95% over 21bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC969992.4515615723618716RNA PCR Primer, Index 20 (95% over 24bp)
ACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCG368940.9324623207529861No Hit
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT294390.7440439708529071No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCGCGATCTCGTAT264360.6681458749776641No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG248420.6278589736039919No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC220760.5579508373432785RNA PCR Primer, Index 31 (95% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC197980.5003764575884321RNA PCR Primer, Index 14 (95% over 22bp)
AGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCT182800.46201038714600157RNA PCR Primer, Index 34 (95% over 22bp)
GAGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTC172460.4358769768446359No Hit
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT165960.4194488175642802RNA PCR Primer, Index 41 (95% over 21bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT165730.4188675134666676RNA PCR Primer, Index 20 (96% over 25bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCGCATCTCGTATG154150.38960011585643406No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC142610.36043381461100266No Hit
CACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCC132550.3350080788632523No Hit
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT130260.32922031197832696RNA PCR Primer, Index 24 (95% over 22bp)
AACTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCC127790.3229776114517918No Hit
GGTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTT121940.30819226809947176RNA PCR Primer, Index 38 (95% over 22bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT116510.29446843657757465TruSeq Adapter, Index 7 (95% over 23bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT110910.28031494550526825RNA PCR Primer, Index 3 (95% over 21bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA110350.27889959639803763RNA PCR Primer, Index 31 (96% over 28bp)
TAACTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGC104770.2647966535081323No Hit
CTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGT100450.25387824610949594No Hit
TGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTC99040.2503145992502188No Hit
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC93360.23595891544830805RNA PCR Primer, Index 38 (100% over 22bp)
GTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAA92170.23295129859544295Illumina PCR Primer Index 7 (96% over 27bp)
AAAACCCATGCGGGTGCGCGGGCGGTGGAGAGGGGGATCTCGTATGCCGT91610.2315359494882123No Hit
AGTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAA87670.22157795755519674RNA PCR Primer, Index 28 (96% over 28bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT86260.21801431069591956No Hit
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA84700.2140715524686342TruSeq Adapter, Index 3 (96% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT83290.2105079056093571RNA PCR Primer, Index 41 (95% over 23bp)
TGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA80180.20264766324598693Illumina PCR Primer Index 3 (96% over 28bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGATCTCGTATGCCGT78790.19913456456911086No Hit
GTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC76950.19448413178821017TruSeq Adapter, Index 3 (96% over 25bp)
GATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA72330.1828075016535574RNA PCR Primer, Index 24 (96% over 30bp)
GATGCCTCCGCTGGCCGGGGATCTCGTATGCCGTCTTCTGCTTGAAAAAA71070.17962296616228848RNA PCR Primer, Index 23 (96% over 28bp)
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTATCTCGTATGCCGTCTTC70680.17863727660546713RNA PCR Primer, Index 37 (95% over 22bp)
CGCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCT68780.17383519927736318No Hit
TGCTGCGATCCATTGAAAGTCAGCCCTCGATCCATCTCGTATGCCGTCTT66470.1679968842100368RNA PCR Primer, Index 42 (95% over 21bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCCATCTCGTATGC65950.16668263146760837No Hit
GCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA64800.16377611097954545RNA PCR Primer, Index 20 (96% over 30bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC64550.16314425869953175RNA PCR Primer, Index 31 (95% over 22bp)
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT64070.1619311023219055Illumina PCR Primer Index 10 (95% over 21bp)
TCAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGT63610.16076849412668032No Hit
AACTGAAAGGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCC62530.15803889227702123No Hit
GGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTGA62180.15715429908500209RNA PCR Primer, Index 38 (96% over 29bp)
GGTGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCCATCTCGTATGCCG61790.15616860952818074No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCCATCTCGTATGCCGTC61620.15573894997777143No Hit
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCATCTCGTATGCC61500.15543566088336486No Hit
GGAGGGTCCTCGCCTGCCGCGCGATGCCTCCGCTGGCCGGGGATCTCGTA61310.15495545315055448No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGATCTCGTATGCCGTCTT61160.15457634178254628No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC60530.1529840740369118No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT59970.15156872492968115No Hit
GGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA59160.14952152354243683Illumina PCR Primer Index 3 (96% over 28bp)
GTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATG58830.1486874785328188No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC58720.14840946352961276No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCTCGTATGCCGTCT58690.14833364125601115Illumina PCR Primer Index 5 (95% over 21bp)
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC58610.14813144852640675RNA PCR Primer, Index 38 (100% over 25bp)
GCCTCCGCTGGCCGGGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA58410.1476259667023958RNA PCR Primer, Index 23 (96% over 28bp)
GGTGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCATCTCGTATGCCGT58070.1467666476015772No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTATCTCGTATGCCGTCTT57750.14595787668315968Illumina PCR Primer Index 10 (95% over 21bp)
CTGAAAGGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGT57690.14580623213595642No Hit
TCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAAA57210.14459307575833014Illumina PCR Primer Index 7 (96% over 27bp)
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC54790.13847674568779775RNA PCR Primer, Index 34 (95% over 24bp)
GTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC52930.13377576472449598Illumina PCR Primer Index 11 (95% over 24bp)
TGAAAGGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTC52860.13359884608609215No Hit
GGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGA51270.12958026558520513RNA PCR Primer, Index 31 (96% over 28bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCGATCTCGTATGC50710.1281649164779745No Hit
TAACTGAAAGGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGC49390.12482873643950229No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCATCTCGTATGCC49360.12475291416590066No Hit
CAAAACCCATGCGGGTGCGCGGGCGGTGGAGAGGGGGATCTCGTATGCCG48890.12356503187947493No Hit
ATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA48770.12326174278506838RNA PCR Primer, Index 26 (96% over 29bp)
TGGCGGCGGCGACCCTGGGCGCGCGCCGCGCCCTTCCCGCGGATCTCGTA48210.12184639367783773No Hit
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA47960.12121454139782406RNA PCR Primer, Index 38 (100% over 27bp)
GTTACTTGGATAACTGTGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA47870.12098707457701913RNA PCR Primer, Index 24 (96% over 29bp)
GAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCT47730.12063323730021147No Hit
GTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA47600.12030467411460435TruSeq Adapter, Index 18 (96% over 28bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC47390.11977391819939286Illumina PCR Primer Index 3 (95% over 22bp)
TTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAA47190.11926843637538193RNA PCR Primer, Index 28 (96% over 28bp)
CGGGAGGGTCCTCGCCTGCCGCGCGATGCCTCCGCTGGCCGGGGATCTCG46970.11871240636896987No Hit
CGCGAAAGGTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCG46300.11701904225853324No Hit
AGCCCTCGATCCAAGTTTTGTATCTCGTATGCCGTCTTCTGCTTGAAAAA45790.11573006360730531Illumina PCR Primer Index 2 (96% over 28bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC45790.11573006360730531TruSeq Adapter, Index 3 (95% over 22bp)
ACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA45620.11530040405689602RNA PCR Primer, Index 20 (96% over 30bp)
TACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTG44490.1124444317512342RNA PCR Primer, Index 38 (96% over 25bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGATCTCGTATGCCGTC43500.10994229672238003No Hit
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCG43420.10974010399277564No Hit
ATTGATCATCGACCTTTCGAACGCATCTCGTATGCCGTCTTCTGCTTGAA43180.10913352580396252Illumina PCR Primer Index 3 (96% over 28bp)
GTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC42370.10708632441671821TruSeq Adapter, Index 3 (96% over 25bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTC42370.10708632441671821TruSeq Adapter, Index 7 (96% over 25bp)
GGAGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGT41510.10491275257347116No Hit
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCGCGATCTCGTATG41330.1044578189318613No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCTCGTATGCCGTCTTC41020.10367432210464435RNA PCR Primer, Index 27 (95% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGT40430.10218315072381207No Hit
TTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCT40260.10175349117340275No Hit
GCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAAA40140.1014502020789962Illumina PCR Primer Index 12 (96% over 26bp)
CCCTGGGCGCGCGCCGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA39870.10076780161658143Illumina PCR Primer Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)