FastQCFastQC Report
Mon 26 Jun 2023
SRR392999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR392999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16638716
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC4487872.6972453884061727RNA PCR Primer, Index 20 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT4361052.621025564713046RNA PCR Primer, Index 31 (95% over 21bp)
ACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCG1520310.9137183422086175No Hit
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT1349920.8113126036888904No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG970990.5835726747184098No Hit
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT767710.4613997858969406RNA PCR Primer, Index 41 (95% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC755240.45390521720546223RNA PCR Primer, Index 31 (95% over 22bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCGCGATCTCGTAT731850.43984764208969007No Hit
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT688020.413505465205368RNA PCR Primer, Index 20 (96% over 25bp)
AGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCT682950.41045835507980305RNA PCR Primer, Index 34 (95% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC682130.4099655285900667RNA PCR Primer, Index 14 (95% over 22bp)
CACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCC666320.4004635934647842No Hit
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT661020.3972782515189273RNA PCR Primer, Index 3 (95% over 21bp)
GAGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTC614730.36945759516539617No Hit
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA584730.35142735773601763TruSeq Adapter, Index 3 (96% over 29bp)
AACTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCC580540.34890913457504774No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC550250.3307046048505185No Hit
CTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGT518620.3116947245208104No Hit
TGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTC501390.301339358157204No Hit
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCCATCTCGTATGC496410.29834633874392713No Hit
TAACTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGC491150.2951850371146428No Hit
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT469580.28222129640291954RNA PCR Primer, Index 24 (95% over 22bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCGCATCTCGTATG441740.26548923606845626No Hit
AAAACCCATGCGGGTGCGCGGGCGGTGGAGAGGGGGATCTCGTATGCCGT438490.2635359603469402No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT437450.2629109121160551TruSeq Adapter, Index 7 (95% over 23bp)
TGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA437350.26285081132462385Illumina PCR Primer Index 3 (96% over 28bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTT411850.2475251095096521RNA PCR Primer, Index 38 (95% over 22bp)
GTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC399690.2402168532716106TruSeq Adapter, Index 3 (96% over 25bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT362330.21776319759289117No Hit
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCGCCCCATCTCGTATGCC360030.21638087938997216No Hit
GGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA331840.19943846628549944Illumina PCR Primer Index 3 (96% over 28bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA314520.18902900920960486RNA PCR Primer, Index 31 (96% over 28bp)
AGTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAA310460.18658891707749564RNA PCR Primer, Index 28 (96% over 28bp)
GTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAA306470.1841908954993883Illumina PCR Primer Index 7 (96% over 27bp)
CCCTGGGCGCGCGCCGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA305510.18361392790164818Illumina PCR Primer Index 1 (96% over 28bp)
CGCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCT300200.18042257587664817No Hit
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC299130.17977949740833365RNA PCR Primer, Index 38 (100% over 22bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGATCTCGTATGCCGT297540.1788238948245766No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT288130.17316841035089486No Hit
GATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA280710.16870893162669523RNA PCR Primer, Index 24 (96% over 30bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC279890.1682161051369589No Hit
CAAAACCCATGCGGGTGCGCGGGCGGTGGAGAGGGGGATCTCGTATGCCG273380.16430354361478375No Hit
TCAGTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGT272500.16377465665018862No Hit
GCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA272300.16365445506732612RNA PCR Primer, Index 20 (96% over 30bp)
GGTGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCCATCTCGTATGCCG270760.16272890287928465No Hit
CTGAAAGGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGT267000.16046911312146922No Hit
TGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCCATCTCGTATGCCGTC266970.16045108288403984No Hit
CGCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCT262640.15784871861506622Illumina PCR Primer Index 3 (96% over 26bp)
AACTGAAAGGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCC261850.15737392236275924No Hit
GTCCGCCCACGGCTGCGCCGCGCCGCCCAGGGCGGGGATCATCTCGTATG258430.15531847529581008No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT255800.1537378244811679RNA PCR Primer, Index 41 (95% over 23bp)
TGAAAGGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTC248660.1494466279729758No Hit
GGTGCGAGAGGTCCCGGGTTCATATCCCGGACGAGCATCTCGTATGCCGT246720.14828067261920932No Hit
GGAGGGTCCTCGCCTGCCGCGCGATGCCTCCGCTGGCCGGGGATCTCGTA242650.14583457040795697No Hit
GATGCCTCCGCTGGCCGGGGATCTCGTATGCCGTCTTCTGCTTGAAAAAA241750.14529366328507562RNA PCR Primer, Index 23 (96% over 28bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC240930.14480083679533926No Hit
CGGGAGGGTCCTCGCCTGCCGCGCGATGCCTCCGCTGGCCGGGGATCTCG234230.1407740837694447No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCATCTCGTATGCC233510.14034135807113962No Hit
CGCGAAAGGTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCG231950.13940378572481193No Hit
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC231270.13899510034307935TruSeq Adapter, Index 3 (95% over 22bp)
GTCCTCGGTTCGTAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC230610.13859843511963302Illumina PCR Primer Index 11 (95% over 24bp)
GTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA229440.13789525585988727TruSeq Adapter, Index 18 (96% over 28bp)
TGCTGCGATCCATTGAAAGTCAGCCCTCGATCCATCTCGTATGCCGTCTT224360.13484213565517916RNA PCR Primer, Index 42 (95% over 21bp)
GAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCT222780.13389254315056523No Hit
TAACTGAAAGGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGC222360.13364011982655394No Hit
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT221190.13293694056680816Illumina PCR Primer Index 10 (95% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC218970.13160270299703417RNA PCR Primer, Index 31 (95% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGATCTCGTATGCCGTCTT218530.1313382595147366No Hit
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCG214720.1290484193612055No Hit
TGGCGGCGGCGACCCTGGGCGCGCGCCGCGCCCTTCCCGCGGATCTCGTA212240.12755791973371022No Hit
GGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTGA210030.12622969224307934RNA PCR Primer, Index 38 (96% over 29bp)
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTATCTCGTATGCCGTCTTC209730.12604938986878556RNA PCR Primer, Index 37 (95% over 22bp)
GTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC201350.1210129435468458TruSeq Adapter, Index 3 (96% over 25bp)
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC201160.12089875204312639RNA PCR Primer, Index 34 (95% over 24bp)
GGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTG199870.12012345183366313TruSeq Adapter, Index 18 (95% over 24bp)
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC199480.1198890587470812RNA PCR Primer, Index 38 (100% over 25bp)
TCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAAA198750.11945032296963298Illumina PCR Primer Index 7 (96% over 27bp)
GCCTCCGCTGGCCGGGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA192960.11597048714576293RNA PCR Primer, Index 23 (96% over 28bp)
CCGCAGCAACCCCGGTTCGTATCCGGGTCACGGCATCTCGTATGCCGTCT189170.1136926671505181RNA PCR Primer, Index 41 (95% over 21bp)
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA189030.11360852604251434RNA PCR Primer, Index 38 (100% over 27bp)
ATTCCCCGACGGGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA187490.1126829738544729RNA PCR Primer, Index 26 (96% over 29bp)
ACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA186540.11211201633587592RNA PCR Primer, Index 20 (96% over 30bp)
CCGCAGCAACCCCGGTTCGAATCCGGGTCACGGCATCTCGTATGCCGTCT184640.11097010129868193RNA PCR Primer, Index 34 (95% over 22bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC184380.11081383924096067Illumina PCR Primer Index 3 (95% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTATCTCGTATGCCGTCTT183760.11044121433408684Illumina PCR Primer Index 10 (95% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGGCTT182020.10939546056318289No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGT180200.10830162615913391No Hit
GTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC179460.10785688030254256RNA PCR Primer, Index 39 (96% over 25bp)
GGTGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGCCATCTCGTATGCCG171360.10298871619661036No Hit
ATTGATCATCGACCTTTCGAACGCATCTCGTATGCCGTCTTCTGCTTGAA167890.10090321873394557Illumina PCR Primer Index 3 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)