FastQCFastQC Report
Mon 26 Jun 2023
SRR393000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR393000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014109
Total Bases217 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCT655372.1743407421563052No Hit
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC517311.7162949316033362Illumina PCR Primer Index 3 (95% over 22bp)
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTT380661.2629271204193346Illumina PCR Primer Index 3 (95% over 21bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG318461.0565643113769276Illumina PCR Primer Index 3 (96% over 28bp)
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT246310.8171900883478335Illumina PCR Primer Index 3 (96% over 27bp)
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCATCTCGTATGCCGTC241160.80010377859593No Hit
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA214680.712250286900706RNA PCR Primer, Index 31 (96% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCATCTCGTATGCCGTCT212110.7037237206749988No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT210460.698249466094292RNA PCR Primer, Index 31 (95% over 21bp)
ACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCG193050.6404877859427114No Hit
AAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCT189190.6276813479539062Illumina PCR Primer Index 3 (96% over 26bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC175980.5838541340077614Illumina PCR Primer Index 3 (96% over 25bp)
AAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTG165330.5485203089868349Illumina PCR Primer Index 3 (95% over 24bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCATCTCGTATGCCGTCTTCT138760.4603682215872087TruSeq Adapter, Index 7 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGATCTCGTATGCCGTCTT120960.40131262671655205No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT108520.36004006490807067TruSeq Adapter, Index 7 (95% over 23bp)
GCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAAA102110.3387734152945365Illumina PCR Primer Index 12 (96% over 26bp)
ACGAGTCTCTGGGCTCCGGGGGGAGTATGGTTGCATCTCGTATGCCGTCT99580.3303795582707858RNA PCR Primer, Index 14 (95% over 21bp)
GAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAA94010.31189980189833877Illumina PCR Primer Index 3 (96% over 28bp)
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT91610.3039372497809469No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCATCTCGTATGCCGTCT90470.30015503752518574No Hit
ATTAAATCAGTTATGGTTCCTTTGATCGCTCCACATCTCGTATGCCGTCT89140.29574245656013104RNA PCR Primer, Index 18 (95% over 21bp)
ATTAAATCAGTTATGGTTCCTTTGATCGCTCCACCATCTCGTATGCCGTC80780.2680062333512159No Hit
TTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTC75410.25019002298855153No Hit
TTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGA73300.24318961258534447Illumina PCR Primer Index 3 (96% over 28bp)
GTCTAGTGGTTAGGATTCGGCGCTCATCTCGTATGCCGTCTTCTGCTTGA70730.23466304635963728TruSeq Adapter, Index 7 (96% over 28bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC70700.2345635144581699No Hit
GTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA70190.23287147213322412Illumina PCR Primer Index 3 (96% over 28bp)
CGGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTT68830.22835935926670206RNA PCR Primer, Index 26 (95% over 22bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT68610.2276294586559411No Hit
TGAAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCT63020.20908334768251577No Hit
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA62250.2065286955448526RNA PCR Primer, Index 38 (100% over 27bp)
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTGCATCTCGTATGCCGTCT59680.1980021293191454No Hit
GTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC57330.19020546370419913TruSeq Adapter, Index 3 (96% over 25bp)
ACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCG56200.18645642874892712No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCTCGTATGCCGTCT54790.1817784293799594Illumina PCR Primer Index 5 (95% over 21bp)
AGAGGGACAAGTGGCGTTCAGCCACGCGAGACTGAGCATCTCGTATGCCG54630.18124759257213324No Hit
GTCGGTAGAGCATGAGACTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA50740.16834162268186054RNA PCR Primer, Index 29 (96% over 28bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC46260.153478192062729No Hit
AAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCT46250.15344501476223987Illumina PCR Primer Index 3 (95% over 23bp)
CGAGTCTCTGGGCTCCGGGGGGAGTATGGTTGCATCTCGTATGCCGTCTT43240.14345864731501085RNA PCR Primer, Index 24 (95% over 23bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG41260.1368895418181625No Hit
TAACTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGC40730.1351311448922385No Hit
AGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAA40430.13413582587756448Illumina PCR Primer Index 3 (96% over 28bp)
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGT40110.13307415226191224No Hit
AAATCAGTTATGGTTCCTTTGATCGCTCCACCATCTCGTATGCCGTCTTC39820.13211201054772737Illumina PCR Primer Index 1 (95% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT38700.12839615289294448No Hit
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCG37740.12521113204598772No Hit
GTTATGGTTCCTTTGATCGCTCCACCATCTCGTATGCCGTCTTCTGCTTG37530.12451440873571594Illumina PCR Primer Index 1 (96% over 28bp)
CGGTTACTTGGATAACTGTGGCATCTCGTATGCCGTCTTCTGCTTGAAAA37250.1235854443220202RNA PCR Primer, Index 24 (96% over 29bp)
GGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGA37120.12315413941566147RNA PCR Primer, Index 31 (96% over 28bp)
GTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC36920.12249059340587881TruSeq Adapter, Index 3 (96% over 25bp)
GTTATGGTTCCTTTGATCGCTCCACATCTCGTATGCCGTCTTCTGCTTGA35630.11821072164278067RNA PCR Primer, Index 18 (96% over 29bp)
AAGGAAAGACTAAAAGAAAGGGAAGGAACTCGGCATCTCGTATGCCGTCT35440.11758035293348715No Hit
ATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCATCTCGTATGCCGTCT33880.11240469405718241No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCTCGTATGCCGTCTTC31940.10596829776229062RNA PCR Primer, Index 27 (95% over 22bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCGCGATCTCGTAT31190.10348000022560565No Hit
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA31170.10341364562462738RNA PCR Primer, Index 38 (100% over 27bp)
AGTTATGGTTCCTTTGATCGCTCCACATCTCGTATGCCGTCTTCTGCTTG31050.10301551801875777TruSeq Adapter, Index 18 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)