FastQCFastQC Report
Mon 26 Jun 2023
SRR393001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR393001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13566734
Total Bases976.8 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCT2332841.7195295492636622No Hit
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC1974391.455317101374583Illumina PCR Primer Index 3 (95% over 22bp)
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTT1575591.1613627863566869Illumina PCR Primer Index 3 (95% over 21bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG1417351.0447245446103683Illumina PCR Primer Index 3 (96% over 28bp)
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT1075510.7927552791998428Illumina PCR Primer Index 3 (96% over 27bp)
ACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCG931590.6866722676216693No Hit
AATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCATCTCGTATGCCGTC846440.6239084513634601No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCATCTCGTATGCCGTCT832550.6136701729391908No Hit
AAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCT818090.6030117491800163Illumina PCR Primer Index 3 (96% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT816700.6019871842405106RNA PCR Primer, Index 31 (95% over 21bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC755230.5566778268078374Illumina PCR Primer Index 3 (96% over 25bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA725500.5347639306556758RNA PCR Primer, Index 31 (96% over 28bp)
AAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTG684510.5045503213964393Illumina PCR Primer Index 3 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGATCTCGTATGCCGTCTT576890.42522393377801904No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCATCTCGTATGCCGTCTTCT537150.39593169586725885TruSeq Adapter, Index 7 (95% over 24bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT497820.36694166775879883TruSeq Adapter, Index 7 (95% over 23bp)
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT480320.3540424688801299No Hit
GCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAAA466060.34353146453671163Illumina PCR Primer Index 12 (96% over 26bp)
ACGAGTCTCTGGGCTCCGGGGGGAGTATGGTTGCATCTCGTATGCCGTCT448020.3302342332355009RNA PCR Primer, Index 14 (95% over 21bp)
GAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAA417780.30794441757316093Illumina PCR Primer Index 3 (96% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCATCTCGTATGCCGTCT373480.27529101698315894No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT372080.27425908107286545No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC368370.27152445091058763No Hit
ATTAAATCAGTTATGGTTCCTTTGATCGCTCCACATCTCGTATGCCGTCT327100.24110445446929232RNA PCR Primer, Index 18 (95% over 21bp)
CGGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTT326870.24093492214117265RNA PCR Primer, Index 26 (95% over 22bp)
GTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC323540.23848038886883166TruSeq Adapter, Index 3 (96% over 25bp)
TTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGA315810.23278262844985387Illumina PCR Primer Index 3 (96% over 28bp)
GTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA310500.22886864296152634Illumina PCR Primer Index 3 (96% over 28bp)
ATTAAATCAGTTATGGTTCCTTTGATCGCTCCACCATCTCGTATGCCGTC294960.2174141543572683No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTGCATCTCGTATGCCGTCT287500.21191541014956142No Hit
GTCTAGTGGTTAGGATTCGGCGCTCATCTCGTATGCCGTCTTCTGCTTGA264670.19508748384098928TruSeq Adapter, Index 7 (96% over 28bp)
TTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTC263760.1944167254992985No Hit
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA262290.19333319279349032RNA PCR Primer, Index 38 (100% over 27bp)
AGAGGGACAAGTGGCGTTCAGCCACGCGAGACTGAGCATCTCGTATGCCG258090.19023738506260976No Hit
TGAAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCT250350.18453225367284418No Hit
GTCGGTAGAGCATGAGACTCATCTCGTATGCCGTCTTCTGCTTGAAAAAA239000.17616620182867887RNA PCR Primer, Index 29 (96% over 28bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC232170.17113182878060407No Hit
CGAGTCTCTGGGCTCCGGGGGGAGTATGGTTGCATCTCGTATGCCGTCTT226380.1668640366944616RNA PCR Primer, Index 24 (95% over 23bp)
ACTTTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCG224090.16517608438405293No Hit
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGT215100.158549581645811No Hit
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCG212810.15686162933540232No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCTCGTATGCCGTCT211060.15557170944753543Illumina PCR Primer Index 5 (95% over 21bp)
TAACTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGC204640.15083954620176088No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT203420.14994028776564797No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG200250.1476036900259119No Hit
CGGTTACTTGGATAACTGTGGCATCTCGTATGCCGTCTTCTGCTTGAAAA196900.14513441481199527RNA PCR Primer, Index 24 (96% over 29bp)
AAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCT194970.1437118174499478Illumina PCR Primer Index 3 (95% over 23bp)
GTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC192830.14213442970135629TruSeq Adapter, Index 3 (96% over 25bp)
AGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAA180980.13339982931780042Illumina PCR Primer Index 3 (96% over 28bp)
GTTATGGTTCCTTTGATCGCTCCACCATCTCGTATGCCGTCTTCTGCTTG164440.12120824363476132Illumina PCR Primer Index 1 (96% over 28bp)
AAATCAGTTATGGTTCCTTTGATCGCTCCACCATCTCGTATGCCGTCTTC158660.11694782251940666Illumina PCR Primer Index 1 (95% over 22bp)
AGTTCTACAGTCCGACGATCGGGCGCTTTGGTGACTCTAGATAACCTCGT147800.1089429482438441No Hit
GTTATGGTTCCTTTGATCGCTCCACATCTCGTATGCCGTCTTCTGCTTGA145850.10750560894022099RNA PCR Primer, Index 18 (96% over 29bp)
AAGGAAAGACTAAAAGAAAGGGAAGGAACTCGGCATCTCGTATGCCGTCT144620.10659897953332026No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCTCGTATGCCGTCTTC143530.10579554371744887RNA PCR Primer, Index 27 (95% over 22bp)
AGTTATGGTTCCTTTGATCGCTCCACATCTCGTATGCCGTCTTCTGCTTG138520.10210268735275564TruSeq Adapter, Index 18 (96% over 29bp)
GTTGGTGGTTCAAGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC136180.10037788018840792Illumina PCR Primer Index 3 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)