FastQCFastQC Report
Mon 26 Jun 2023
SRR393002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR393002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4037991
Total Bases290.7 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC3547198.784541619830257RNA PCR Primer, Index 20 (95% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT1223033.0288081375119456RNA PCR Primer, Index 31 (95% over 21bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC938672.3245965629938254RNA PCR Primer, Index 14 (95% over 22bp)
GCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAAA754961.8696426019770722Illumina PCR Primer Index 12 (96% over 26bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT602141.4911870779305847RNA PCR Primer, Index 3 (95% over 21bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT553251.3701120185755737RNA PCR Primer, Index 20 (96% over 25bp)
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC475431.1773924211321916TruSeq Adapter, Index 3 (95% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT460111.1394527625247306RNA PCR Primer, Index 41 (95% over 23bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT430321.0656784524779772TruSeq Adapter, Index 11 (95% over 24bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC341920.8467577069884504No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT293640.7271932998364781No Hit
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT283770.7027504518955094RNA PCR Primer, Index 41 (95% over 21bp)
CGCAGCAACCCCGGTTCGAATCCGGGTCACGGCATCTCGTATGCCGTCTT278130.688783110214956RNA PCR Primer, Index 41 (95% over 22bp)
CGCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAA242710.6010662232778627Illumina PCR Primer Index 12 (96% over 26bp)
CGCAGCAACCCCGGTTCGTATCCGGGTCACGGCATCTCGTATGCCGTCTT215740.534275584071386RNA PCR Primer, Index 34 (95% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC190280.47122442818718513TruSeq Adapter, Index 18 (95% over 22bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA175940.4357117190206714TruSeq Adapter, Index 3 (96% over 29bp)
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT172430.42701927765564607No Hit
GTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA161180.3991588886651803Illumina PCR Primer Index 3 (96% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG158380.392224747405331TruSeq Adapter, Index 3 (96% over 26bp)
ACAGTCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGCCG146210.36208599771520045No Hit
TTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA144810.3586189270852758Illumina PCR Primer Index 12 (96% over 26bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGTATGCCGTCTTCTGCT144180.35705874530180975No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCT140520.34799483208357823Illumina PCR Primer Index 3 (95% over 24bp)
GCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA138690.3434628754744624RNA PCR Primer, Index 20 (96% over 30bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGATCTCGTATGCCGTCTTCTG135700.33605820320055196TruSeq Adapter, Index 9 (95% over 24bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC128180.31743508095981393TruSeq Adapter, Index 3 (96% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC123770.3065138084755513RNA PCR Primer, Index 41 (95% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC116190.2877420974935308RNA PCR Primer, Index 31 (95% over 22bp)
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGT111690.2765979418973445No Hit
AGTTCTACAGTCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGT110710.27417099245639726No Hit
CTACAGTCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGC105690.2617390677690961No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC104020.2576033478034003No Hit
AGTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAA100690.2493566726622224RNA PCR Primer, Index 28 (96% over 28bp)
AGTCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGCCGTC96830.23979746363971588No Hit
ACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGC96030.2378162804226161Illumina PCR Primer Index 12 (95% over 23bp)
ACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGCT94490.23400250272969902RNA PCR Primer, Index 21 (96% over 26bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGATCTCGTATGCCGT94210.23330908860371408No Hit
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC91850.2274645981132697Illumina PCR Primer Index 3 (95% over 22bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGATCTCGTATGCCGTCTTCTG87300.21619661856601466RNA PCR Primer, Index 23 (95% over 24bp)
GTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAA86650.21458690720212106Illumina PCR Primer Index 7 (96% over 27bp)
TTCTACAGTCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTAT86270.21364584517399865No Hit
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC83410.2065631151728669RNA PCR Primer, Index 34 (95% over 24bp)
CGCAGCAACCCCGGTTCGGATCCGGGTCACGGCATCTCGTATGCCGTCTT83290.20626593769030194RNA PCR Primer, Index 41 (95% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC81910.20284839664080478RNA PCR Primer, Index 31 (95% over 22bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTATCTCGTATGCCGTCTTC78580.19460172149962693TruSeq Adapter, Index 10 (96% over 25bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG77500.1919271241565422Illumina PCR Primer Index 3 (96% over 28bp)
GTTCTACAGTCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTA77120.1909860621284198No Hit
TACAGTCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGCC76480.18940111555473998No Hit
TCTACAGTCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATG75050.18585975055417409No Hit
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCT74190.18372997859579182No Hit
CCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCT74110.18353186027408186TruSeq Adapter, Index 3 (95% over 23bp)
TACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGC71520.17711777960872127RNA PCR Primer, Index 33 (100% over 24bp)
AGGCCGTGGGGAGGAAAGGGGTGGATCGTGGCATCTCGTATGCCGTCTTC71110.17610242320995761RNA PCR Primer, Index 24 (95% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCATCTCGTATGCCGTCTTCT70910.17560712740568268TruSeq Adapter, Index 18 (96% over 29bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCATCTCGTATGCCGTCTTCT69390.17184287929319308TruSeq Adapter, Index 3 (95% over 24bp)
GTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC69200.17137234827913186TruSeq Adapter, Index 3 (96% over 25bp)
GTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGCTTGA69150.17124852432806315TruSeq Adapter, Index 3 (96% over 28bp)
CTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTG67370.1668403916700161RNA PCR Primer, Index 33 (100% over 23bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCT67060.16607268317338994Illumina PCR Primer Index 3 (95% over 24bp)
CCGCAGCAACCCCGGTTCGAATCCGGGTCACGGCATCTCGTATGCCGTCT66780.16537926904740502RNA PCR Primer, Index 34 (95% over 22bp)
CAGTCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGCCGT65990.16342285062051898No Hit
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTC64980.16092160680893047No Hit
TCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAAA62280.1542351134512187Illumina PCR Primer Index 7 (96% over 27bp)
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT61690.1527739908286076Illumina PCR Primer Index 10 (95% over 21bp)
AGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGCTTG61380.15200628233198143RNA PCR Primer, Index 21 (96% over 28bp)
TTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA61080.151263338625569RNA PCR Primer, Index 29 (96% over 26bp)
GATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTT60820.15061945408001157Illumina PCR Primer Index 12 (96% over 25bp)
TGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTC60410.14960409768124794No Hit
CGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGTATGCCGTCTTCTGC59130.14643420453388825No Hit
AGTTCTACAGTCCGACGATCAAACTCTAGATAACCTCGTATGCCGTCTTC59010.14613702705132328No Hit
TCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTT58670.14529502418405588No Hit
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT58470.14479972837978095Illumina PCR Primer Index 3 (96% over 27bp)
CGCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCT57500.14239754372904742Illumina PCR Primer Index 3 (96% over 26bp)
ACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA56740.14051541967280265RNA PCR Primer, Index 20 (96% over 30bp)
GGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCTTCTGCTTGAAA56440.13977247596639022Illumina PCR Primer Index 3 (96% over 28bp)
GGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGAA55980.13863329561655785RNA PCR Primer, Index 41 (96% over 28bp)
CGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCT54680.1354138728887707Illumina PCR Primer Index 12 (95% over 21bp)
TCTACAGTCCGACGATCAAACTCTAGATAACCTCGTATGCCGTCTTCTGC54070.1339032206857321RNA PCR Primer, Index 29 (95% over 23bp)
CCGCAGCAACCCCGGTTCGTATCCGGGTCACGGCATCTCGTATGCCGTCT52410.12979226551025003RNA PCR Primer, Index 41 (95% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGATCTCGTATGCCGTCTTC51380.12724149211823405RNA PCR Primer, Index 21 (95% over 22bp)
TCGCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAA51250.12691954984545534Illumina PCR Primer Index 12 (96% over 26bp)
GTTGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA50680.12550795680327173Illumina PCR Primer Index 3 (96% over 29bp)
CGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTG50260.12446783561429434RNA PCR Primer, Index 34 (95% over 24bp)
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT49860.12347724400574443RNA PCR Primer, Index 24 (95% over 22bp)
GAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCTTCT48900.12109982414522469Illumina PCR Primer Index 3 (95% over 23bp)
CCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTC48710.1206292931311635No Hit
CATCCCGGGGCCACGCCTGTCTGAGGGCCGCCATCTCGTATGCCGTCTTC47860.11852428596299497RNA PCR Primer, Index 20 (100% over 21bp)
GTCCGACGATCAAACGCTTTGGTGACTCTAGATAACCTCGTATGCCGTCT47550.1177565774663688No Hit
CAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGCTT47130.11671645627739141RNA PCR Primer, Index 33 (100% over 26bp)
CGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA46820.11594874778076525RNA PCR Primer, Index 41 (96% over 28bp)
TTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAA46460.11505721533307033RNA PCR Primer, Index 28 (96% over 28bp)
TTCTACAGTCCGACGATCAAACTCTAGATAACCTCGTATGCCGTCTTCTG46060.11406662372452044TruSeq Adapter, Index 12 (95% over 22bp)
GAGGCCGTGGGGAGGAAAGGGGTGGATCGTGGCATCTCGTATGCCGTCTT45450.11255597152148185RNA PCR Primer, Index 34 (95% over 23bp)
GGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCT44550.11032714040224458RNA PCR Primer, Index 24 (95% over 24bp)
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCG44310.10973278543711465No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG43310.10725630641573991Illumina PCR Primer Index 10 (96% over 26bp)
GGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGA42880.10619142043654876RNA PCR Primer, Index 20 (96% over 30bp)
GTACTCTGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA42200.10450741470201395RNA PCR Primer, Index 21 (96% over 28bp)
GGCTTTGGTCTAGGGGTATGATTCTCGCTTATCTCGTATGCCGTCTTCTG41460.10267482022619663TruSeq Adapter, Index 10 (96% over 27bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA41380.10247670190448666RNA PCR Primer, Index 31 (96% over 28bp)
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC41350.10240240753384541RNA PCR Primer, Index 38 (100% over 22bp)
GGCCGTGGGGAGGAAAGGGGTGGATCGTGGCATCTCGTATGCCGTCTTCT41330.10235287795341792RNA PCR Primer, Index 34 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)