FastQCFastQC Report
Mon 26 Jun 2023
SRR393003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR393003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11517656
Total Bases829.2 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC208723118.122011978826247RNA PCR Primer, Index 14 (95% over 22bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC180580715.678598145317068RNA PCR Primer, Index 20 (95% over 24bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT6847255.945003045758616TruSeq Adapter, Index 11 (95% over 24bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT3187472.7674641437459147No Hit
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT3021572.623424418996365RNA PCR Primer, Index 20 (96% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT2417252.098734325803792RNA PCR Primer, Index 41 (95% over 23bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC2383372.069318618302196No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC1064180.9239553603615179RNA PCR Primer, Index 41 (95% over 22bp)
GAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTCTTC1004410.8720611207697121RNA PCR Primer, Index 20 (95% over 23bp)
CTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTG996860.8655059675336718RNA PCR Primer, Index 33 (100% over 23bp)
GAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCTTCT978210.849313436692327Illumina PCR Primer Index 3 (95% over 23bp)
TACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGC939990.8161296013702788RNA PCR Primer, Index 33 (100% over 24bp)
ACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGCT871570.7567251531040691RNA PCR Primer, Index 21 (96% over 26bp)
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGT800990.6954453232498001No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT753770.6544473979775052RNA PCR Primer, Index 31 (95% over 21bp)
CCATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTT732260.6357717229964153RNA PCR Primer, Index 41 (95% over 21bp)
CGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTCT629850.5468560616847733RNA PCR Primer, Index 20 (95% over 23bp)
GGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCTTCTGC478890.4157877262526333Illumina PCR Primer Index 3 (96% over 25bp)
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCG429140.3725931734721023No Hit
GGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTCTTCTG403510.35034038175823273RNA PCR Primer, Index 20 (96% over 25bp)
GCGTTGGTGGTATAGTGGTTAGCATAGCTGCCATCTCGTATGCCGTCTTC399390.34676326502545307RNA PCR Primer, Index 14 (95% over 22bp)
GGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTG395070.34301250184933463RNA PCR Primer, Index 41 (96% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG374410.32507482425243467Illumina PCR Primer Index 10 (96% over 26bp)
AGTTCTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCT371220.32230516348118055No Hit
AGGCCGTGGGGAGGAAAGGGGTGGATCGTGGCATCTCGTATGCCGTCTTC322470.2799788429173436RNA PCR Primer, Index 24 (95% over 23bp)
CGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTCTT307060.2665993844580876RNA PCR Primer, Index 20 (100% over 21bp)
CAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGCTT293840.25512135455339174RNA PCR Primer, Index 33 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCATCTCGTATGCCGTCTTCT289910.251709201941784TruSeq Adapter, Index 18 (96% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC269950.23437928689656992TruSeq Adapter, Index 18 (95% over 22bp)
CGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCTTC269440.23393648846605591Illumina PCR Primer Index 3 (95% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGATCTCGTATGCCGTCTTCTG253400.2200100437102827TruSeq Adapter, Index 9 (95% over 24bp)
GGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGA250740.21770054601387645RNA PCR Primer, Index 20 (96% over 30bp)
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC250270.21729247687203018RNA PCR Primer, Index 34 (95% over 24bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTATCTCGTATGCCGTCTTCTGC222560.19323376214743695RNA PCR Primer, Index 38 (100% over 26bp)
CCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCT220810.1917143557682223RNA PCR Primer, Index 41 (96% over 26bp)
GTGGCCTACGTCCTCCCAAGTGCAGACCGCATCTCGTATGCCGTCTTCTG218590.1897868802471614RNA PCR Primer, Index 30 (96% over 25bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCATCTCGTATGCCGTCTTCT203920.1770499136282591TruSeq Adapter, Index 11 (95% over 23bp)
AGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGCTTG203830.1769717727287566RNA PCR Primer, Index 21 (96% over 28bp)
AGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTCTTCT201750.17516584971803292RNA PCR Primer, Index 20 (100% over 23bp)
CGGTGGCCTACGTCCTCCCAAGTGCAGACCGCATCTCGTATGCCGTCTTC196370.1704947603922187TruSeq Adapter, Index 18 (95% over 24bp)
CCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGC188630.1637746430350064RNA PCR Primer, Index 20 (96% over 27bp)
CGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTT176730.15344267965634675RNA PCR Primer, Index 20 (96% over 29bp)
GAGGCCGTGGGGAGGAAAGGGGTGGATCGTGGCATCTCGTATGCCGTCTT167820.14570673060560238RNA PCR Primer, Index 34 (95% over 23bp)
GTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTT165200.14343196219786386RNA PCR Primer, Index 31 (96% over 27bp)
GTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGC157170.13646005749781032TruSeq Adapter, Index 18 (96% over 25bp)
GCGTTGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTCT156770.13611276461113267RNA PCR Primer, Index 14 (95% over 23bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCATCTCGTATGCCGTCTTCT156090.1355223667037807TruSeq Adapter, Index 3 (95% over 24bp)
GTTCTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTT146170.12690950311417531No Hit
TCTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCT141730.12305455207205354RNA PCR Primer, Index 33 (100% over 22bp)
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT141060.12247283648686852Illumina PCR Primer Index 10 (95% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG140540.12202135573418757TruSeq Adapter, Index 3 (96% over 26bp)
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTC140470.12196057947901899No Hit
CCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCTT137650.11951216462794166RNA PCR Primer, Index 34 (95% over 22bp)
CGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTG136520.11853106222307734TruSeq Adapter, Index 11 (96% over 25bp)
AGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCTTCTG134740.11698560887736185RNA PCR Primer, Index 34 (96% over 25bp)
CCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTCT124980.10851166244242752RNA PCR Primer, Index 20 (95% over 21bp)
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT120620.10472616997764128RNA PCR Primer, Index 24 (95% over 22bp)
GTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTCTG117070.10164394560837726RNA PCR Primer, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)