FastQCFastQC Report
Mon 26 Jun 2023
SRR393004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR393004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459729
Total Bases177.1 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG845233.436272857700991Illumina PCR Primer Index 3 (96% over 28bp)
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT497062.020791721364427Illumina PCR Primer Index 3 (96% over 27bp)
GCTTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAAA419571.7057570163217166Illumina PCR Primer Index 12 (96% over 26bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC385761.568302849622865Illumina PCR Primer Index 3 (95% over 22bp)
TTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA354841.442597944732936Illumina PCR Primer Index 12 (96% over 26bp)
TTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGA321481.3069732478659235Illumina PCR Primer Index 3 (96% over 28bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCT317961.2926627282924257Illumina PCR Primer Index 3 (96% over 26bp)
ATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGA283461.152403374518087RNA PCR Primer, Index 30 (96% over 29bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC246681.0028747069290966Illumina PCR Primer Index 3 (96% over 25bp)
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA218630.8888377540777866RNA PCR Primer, Index 38 (100% over 27bp)
AAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTG201940.8209847507591285Illumina PCR Primer Index 3 (95% over 24bp)
AATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTG191200.7773214041058994RNA PCR Primer, Index 30 (100% over 28bp)
GTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA174220.7082894091178337Illumina PCR Primer Index 3 (96% over 28bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCT165600.6732448981168251RNA PCR Primer, Index 30 (95% over 24bp)
AGTTCTACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATG149930.6095386930836689No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT145940.5933173939080281RNA PCR Primer, Index 31 (95% over 21bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC136870.5564434130751803No Hit
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTT134360.5462390369020328Illumina PCR Primer Index 3 (95% over 21bp)
AGTTCTACAGTCCGACGATCGGGCGCTTTGGTGACTCTAGATAACCTCGT130130.5290420204827442No Hit
TTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGAA127330.5176586526401892RNA PCR Primer, Index 30 (100% over 28bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTT126600.5146908460240945RNA PCR Primer, Index 30 (96% over 28bp)
TCTACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATGCCG125230.5091211267582729No Hit
ACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATGCCGTCT120740.4908670833250329No Hit
TACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATGCCGTC115480.4694826137350903No Hit
GAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAA115230.4684662416062908Illumina PCR Primer Index 3 (96% over 28bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGATCTCGTATGCCGTCTTCTG109450.4449677179884451RNA PCR Primer, Index 23 (95% over 24bp)
TCTACAGTCCGACGATCGGGCGCTTTGGTGACTCTAGATAACCTCGTATG108640.44167467229113455No Hit
TTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCT108160.43972323780383943No Hit
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA107640.43760918377593633RNA PCR Primer, Index 38 (100% over 27bp)
ACAGTCCGACGATCGGGCGCTTTGGTGACTCTAGATAACCTCGTATGCCG104180.42354259351335044No Hit
GTTCTACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATGC103600.4211846101745355No Hit
TACAGTCCGACGATCGGGCGCTTTGGTGACTCTAGATAACCTCGTATGCC101950.41447655412445844No Hit
AAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCT100080.40687409060103774RNA PCR Primer, Index 30 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA98420.40012537966580874RNA PCR Primer, Index 29 (96% over 26bp)
GTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC96500.3923196417166281TruSeq Adapter, Index 3 (96% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG95670.3889452862490136TruSeq Adapter, Index 18 (95% over 24bp)
CTACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATGCCGT95650.3888639764787097No Hit
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT94990.3861807540586788No Hit
TAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAA93910.38179002646226473Illumina PCR Primer Index 3 (96% over 28bp)
GGCGACCCTGGGCGCGCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA88630.36032424710201816RNA PCR Primer, Index 20 (100% over 27bp)
TTCTACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATGCC85120.3460543824136724No Hit
CTACAGTCCGACGATCGGGCGCTTTGGTGACTCTAGATAACCTCGTATGC82700.3362159002068927No Hit
GTTCTACAGTCCGACGATCGGGCGCTTTGGTGACTCTAGATAACCTCGTA81100.32971111858257557No Hit
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA80020.3253203909861615RNA PCR Primer, Index 31 (96% over 28bp)
AAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGC80000.32523908121585754RNA PCR Primer, Index 30 (96% over 26bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCT72730.29568297971036644Illumina PCR Primer Index 3 (95% over 24bp)
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTC72440.294503988040959RNA PCR Primer, Index 30 (100% over 22bp)
TTCTACAGTCCGACGATCGGGCGCTTTGGTGACTCTAGATAACCTCGTAT71430.29039784464060875No Hit
GTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA64720.26311841670362873RNA PCR Primer, Index 30 (100% over 28bp)
GGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA64280.26132960175694153Illumina PCR Primer Index 3 (96% over 28bp)
TGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA61460.24986492414408257Illumina PCR Primer Index 3 (96% over 28bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC61440.24978361437377858RNA PCR Primer, Index 20 (95% over 24bp)
GGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA59540.24205918619490197RNA PCR Primer, Index 31 (96% over 28bp)
GTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC58580.23815631722031166TruSeq Adapter, Index 3 (96% over 25bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA54080.21986161890191971TruSeq Adapter, Index 3 (96% over 29bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT53970.21941441516524787No Hit
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT53550.2177069099888646RNA PCR Primer, Index 20 (96% over 25bp)
AAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCT53400.2170970867115849Illumina PCR Primer Index 3 (95% over 23bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC52820.2147391033727699No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT51190.20811235709299686No Hit
GGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGAA48520.19725750275741757RNA PCR Primer, Index 41 (96% over 28bp)
GTTGGTGGTTCAAGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC45970.18689050704366214Illumina PCR Primer Index 3 (96% over 25bp)
GAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGAAAAA45100.18335353203543966RNA PCR Primer, Index 30 (100% over 28bp)
AGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAA44640.1814834073184485Illumina PCR Primer Index 3 (96% over 28bp)
TTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTT44240.1798572119123692RNA PCR Primer, Index 30 (100% over 21bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT44050.17908476909448154TruSeq Adapter, Index 11 (95% over 24bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGATCTCGTATGCCGT43290.1759949978229309No Hit
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC39010.15859470697788253TruSeq Adapter, Index 3 (95% over 22bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT38670.15721244088271513TruSeq Adapter, Index 7 (95% over 23bp)
GTTCAGAGTTCTACAGTCCGACGATCGGGCTGTAGAACTCTGAACTCGTA38270.15558624547663583Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA37860.15391939518540457Illumina PCR Primer Index 3 (96% over 29bp)
AGTTCTACAGTCCGACGATCGGGCTTTGGTGACTCTAGATAACCTCGTAT37570.15274040351599708No Hit
TAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGAAA37150.15103289833961384RNA PCR Primer, Index 30 (96% over 29bp)
GTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA35180.14302388596467336TruSeq Adapter, Index 18 (96% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT33910.1378607155503716RNA PCR Primer, Index 41 (95% over 23bp)
GTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGC32730.13306343910243773TruSeq Adapter, Index 18 (96% over 25bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCATCTCGTATGCCGTCTTCT32670.1328195097915258TruSeq Adapter, Index 7 (95% over 24bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTC32200.13090873018938265TruSeq Adapter, Index 7 (96% over 25bp)
GGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA31290.12720913564055225TruSeq Adapter, Index 18 (96% over 28bp)
TCTACAGTCCGACGATCGGGCTTTGGTGACTCTAGATAACCTCGTATGCC31220.12692455144448841No Hit
ATGGTTAGCACTCTGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA30680.12472918764628135Illumina PCR Primer Index 8 (96% over 29bp)
ACAGTCCGACGATCGGGCTTTGGTGACTCTAGATAACCTCGTATGCCGTC29410.11956601723197961No Hit
TACAGTCCGACGATCGGGCTTTGGTGACTCTAGATAACCTCGTATGCCGT28110.11428088216222194No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGATCTCGTATGCCGTCTTCTG27360.11123176577582326Illumina PCR Primer Index 3 (95% over 23bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT27180.1104999778430876RNA PCR Primer, Index 3 (95% over 21bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCT26790.10891443732216029Illumina PCR Primer Index 3 (95% over 24bp)
GTTCTACAGTCCGACGATCGGGCTTTGGTGACTCTAGATAACCTCGTATG25930.10541811719908982No Hit
CAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATGCCGTCTT24860.10106804448782772No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC24690.10037691144024402TruSeq Adapter, Index 18 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)