FastQCFastQC Report
Mon 26 Jun 2023
SRR393005.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR393005.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9836111
Total Bases708.1 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC5480995.572314098529389Illumina PCR Primer Index 3 (95% over 22bp)
AAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTG2620662.664325361924037Illumina PCR Primer Index 3 (95% over 24bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC2473802.5150183848067593Illumina PCR Primer Index 3 (96% over 25bp)
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTT2031372.0652166288078693Illumina PCR Primer Index 3 (95% over 21bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCT1616381.643312077303723RNA PCR Primer, Index 30 (95% over 24bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCT1565311.5913911504252036Illumina PCR Primer Index 3 (96% over 26bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC1484661.5093973624331811No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT1445461.4695442131549754RNA PCR Primer, Index 31 (95% over 21bp)
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT1235401.2559841994463055No Hit
TTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCT1195391.2153075539712799No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT839480.8534673917364292No Hit
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT818810.8324529887879468Illumina PCR Primer Index 3 (96% over 27bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT817240.8308568294928758No Hit
TGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTC796390.8096594273895444RNA PCR Primer, Index 30 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG774310.7872115310614124TruSeq Adapter, Index 18 (95% over 24bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC723870.7359311012248642No Hit
AAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCT708960.7207726712315468Illumina PCR Primer Index 3 (95% over 23bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC613510.6237322860630589RNA PCR Primer, Index 20 (95% over 24bp)
AAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGC608790.618933641558132RNA PCR Primer, Index 30 (96% over 26bp)
TTGAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTT533620.5425111611692873RNA PCR Primer, Index 30 (100% over 21bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCT508160.5166269473778813RNA PCR Primer, Index 30 (100% over 26bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG482680.4907224003470477Illumina PCR Primer Index 3 (96% over 28bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTT422450.42948884981066193TruSeq Adapter, Index 7 (95% over 23bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT410460.4172990727737823TruSeq Adapter, Index 11 (95% over 24bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT394700.4012764801047894RNA PCR Primer, Index 20 (96% over 25bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGATCTCGTATGCCGT393710.4002699847531204No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT352260.3581293460393036RNA PCR Primer, Index 41 (95% over 23bp)
GGTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTC316720.321997179576359TruSeq Adapter, Index 7 (96% over 25bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGATCTCGTATGCCGTCTTCTG301380.30640158493534686RNA PCR Primer, Index 23 (95% over 24bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT290840.29568596775697226RNA PCR Primer, Index 3 (95% over 21bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTT277050.2816661991716035RNA PCR Primer, Index 30 (96% over 28bp)
TGAAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCT276720.28133070072104716No Hit
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC272550.2770912203003809TruSeq Adapter, Index 3 (95% over 22bp)
TGGGCTCGAGCCGCGACTGGGGGAGCGGCCGCCCCGAGGTGCATCTCGTA255700.25996046608258083No Hit
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT253830.25805930819609496Illumina PCR Primer Index 10 (95% over 21bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGGC252410.25661564819673144No Hit
AGTTCTACAGTCCGACGATCGGGCGTACTCTGCGATCTCGTATGCCGTCT250830.25500932228194656No Hit
AAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC248790.2529353318603257Illumina PCR Primer Index 3 (95% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC241640.2456661987649387No Hit
GCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGTC240570.24457837045555908No Hit
AGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTC233800.23769556890929758RNA PCR Primer, Index 34 (95% over 24bp)
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGT225400.22915560834968213No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC221500.2251906266612892TruSeq Adapter, Index 18 (95% over 22bp)
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT221120.2248042951121637RNA PCR Primer, Index 24 (95% over 22bp)
GCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCT217200.22081898018434318No Hit
ACGAGTCTCTGGGCTCCGGGGGGAGTATGGTTGCATCTCGTATGCCGTCT216000.2195989858186838RNA PCR Primer, Index 14 (95% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTATCTCGTATGCCGTCTTC210980.2144953427223422RNA PCR Primer, Index 27 (95% over 22bp)
ACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCG199850.2031798949808517No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCATCTCGTATGCCGTCTTCT193280.1965004258288667TruSeq Adapter, Index 7 (95% over 24bp)
GTTGGTGGTTCAAGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC191880.1950770990689308Illumina PCR Primer Index 3 (96% over 25bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCATCTCGTATGCCG186210.18931262569119034No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCT182160.18519514470709003Illumina PCR Primer Index 3 (95% over 24bp)
GTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC181550.18457498090454652TruSeq Adapter, Index 3 (96% over 25bp)
CGCAGCAACCCCGGTTCGAATCCGGGTCACGGCATCTCGTATGCCGTCTT172250.17512002457068654RNA PCR Primer, Index 41 (95% over 22bp)
AAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTG167490.17028071358690441RNA PCR Primer, Index 30 (100% over 24bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCATCTCGTATGCC164020.16675289654620612No Hit
CTGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCG160940.1636215776743471No Hit
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCATCTCGTATGCCGTCTTCT152080.15461395260789554TruSeq Adapter, Index 3 (95% over 24bp)
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCTCGTATGCCGTCT148680.15115730190519402Illumina PCR Primer Index 5 (95% over 21bp)
AGTTCTACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATG137730.1400248533185524No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG136710.13898785810774197No Hit
GTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC132690.13490087698278314TruSeq Adapter, Index 3 (96% over 25bp)
TGAAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTT130470.13264388740631333RNA PCR Primer, Index 30 (100% over 21bp)
GTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGC128890.1310375614915285TruSeq Adapter, Index 18 (96% over 25bp)
GAGGCCGTGGGGAGGAAAGGGGTGGATCGTGGCATCTCGTATGCCGTCTT127930.13006156599900104RNA PCR Primer, Index 34 (95% over 23bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGATCTCGTATGCCGTCT126360.12846540670393003No Hit
GGGCTGAGCCGGTCGGGCTGAGGTGCGAAGCGGGCATCTCGTATGCCGTC125520.1276114106479685No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC124930.12701158008485264RNA PCR Primer, Index 31 (95% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTTCTG124870.12695058036656967Illumina PCR Primer Index 7 (100% over 24bp)
AATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTG124830.12690991388771436RNA PCR Primer, Index 30 (100% over 28bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCATCTCGTATGCCGTCTTCT123800.12586275205719008TruSeq Adapter, Index 7 (96% over 25bp)
ATTAAATCAGTTATGGTTCCTTTGATCGCTCCATCTCGTATGCCGTCTTC121100.12311776473445653TruSeq Adapter, Index 11 (95% over 23bp)
GTTCTACAGTCCGACGATCGGGCGTACTCTGCGATCTCGTATGCCGTCTT120760.1227720996641864RNA PCR Primer, Index 21 (95% over 21bp)
GCAGCAACCCCGGTTCGAATCCGGGTCACGGCATCTCGTATGCCGTCTTC119940.12193843684765249RNA PCR Primer, Index 34 (95% over 24bp)
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGTC118710.12068794262285165No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCATCTCGTATGCCGTCTTCT116660.11860378558151692TruSeq Adapter, Index 18 (96% over 29bp)
GTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCT116380.11831912022952974TruSeq Adapter, Index 18 (95% over 23bp)
GAAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTT116170.11810562121553934Illumina PCR Primer Index 3 (95% over 21bp)
TTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGA113450.11534030065337815Illumina PCR Primer Index 3 (96% over 28bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCT110820.11266647966864139RNA PCR Primer, Index 31 (95% over 23bp)
AGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCT109850.11168031755640008RNA PCR Primer, Index 34 (95% over 22bp)
GAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCTTCT109130.11094832093700448Illumina PCR Primer Index 3 (95% over 23bp)
TGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTC108690.11050098966959604No Hit
GCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTCT106960.1087421644591038No Hit
TCTACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATGCCG104980.10672917375576588No Hit
GTTACTTGGATAACTGTGGCAATTCCAGAGCATCTCGTATGCCGTCTTCT104800.10654617460091698RNA PCR Primer, Index 26 (95% over 24bp)
TACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATGCCGTC102870.10458401699614817No Hit
ACAGTCCGACGATCGGGCTTGGTGACTCTAGATAACCTCGTATGCCGTCT100020.10168653037770721No Hit
TGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTG98760.10040553629376489TruSeq Adapter, Index 18 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)