FastQCFastQC Report
Mon 26 Jun 2023
SRR393006.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR393006.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4492941
Total Bases323.4 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT1424853.1713080585745503RNA PCR Primer, Index 31 (95% over 21bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT1119822.4923986315422346TruSeq Adapter, Index 11 (95% over 24bp)
AGTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAA1028442.289012920490164RNA PCR Primer, Index 28 (96% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC747241.6631422491414867RNA PCR Primer, Index 14 (95% over 22bp)
GTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAA677801.508588695021813Illumina PCR Primer Index 7 (96% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT412780.9187300701255593RNA PCR Primer, Index 41 (95% over 23bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC374960.8345535808282369RNA PCR Primer, Index 20 (95% over 24bp)
TCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAAA338960.7544278903284063Illumina PCR Primer Index 7 (96% over 27bp)
TTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAA300450.6687156586298373RNA PCR Primer, Index 28 (96% over 28bp)
CTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAAAA245020.545344352396348RNA PCR Primer, Index 28 (96% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG233150.5189251316676537Illumina PCR Primer Index 10 (96% over 26bp)
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA209700.46673214716151407RNA PCR Primer, Index 38 (100% over 27bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAA188630.4198363610828631Illumina Paired End PCR Primer 2 (100% over 24bp)
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA176280.39234879781417115RNA PCR Primer, Index 38 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG160550.35733832249299513TruSeq Adapter, Index 18 (95% over 24bp)
GAGTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGA137790.30668108038810216RNA PCR Primer, Index 28 (96% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC122670.2730282903781732TruSeq Adapter, Index 18 (95% over 22bp)
GATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA122210.27200446211067536TruSeq Adapter, Index 18 (100% over 27bp)
TACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAA106390.2367936725632498Illumina PCR Primer Index 7 (96% over 27bp)
TGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA105290.23434538757575496Illumina PCR Primer Index 3 (96% over 28bp)
GCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCT102350.22780178951826877RNA PCR Primer, Index 3 (96% over 27bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA100180.22297199095202896RNA PCR Primer, Index 31 (96% over 28bp)
ACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGCT99590.22165881991328173RNA PCR Primer, Index 21 (96% over 26bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTATCTCGTATGCCGTCTTC97960.2180309067045394TruSeq Adapter, Index 10 (96% over 25bp)
CGCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGC97550.21711836411829133TruSeq Adapter, Index 3 (96% over 26bp)
ACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA95410.21235533696080142RNA PCR Primer, Index 28 (96% over 28bp)
GTACTCTGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA94340.20997382338205645RNA PCR Primer, Index 21 (96% over 28bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG85230.18969757225834927No Hit
GGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGA77620.17275989157213503RNA PCR Primer, Index 31 (96% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG77420.172314748847136TruSeq Adapter, Index 3 (96% over 26bp)
TGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGC75750.16859780709339384RNA PCR Primer, Index 38 (100% over 24bp)
GTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTT75370.16775203591589563RNA PCR Primer, Index 24 (96% over 28bp)
CCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCT74440.16568212224465RNA PCR Primer, Index 31 (95% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC74200.16514795097465113RNA PCR Primer, Index 31 (95% over 22bp)
TACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGC73600.16381252279965394RNA PCR Primer, Index 33 (100% over 24bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT71520.15918303845966372RNA PCR Primer, Index 20 (96% over 25bp)
CTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTG71420.15896046709716422RNA PCR Primer, Index 33 (100% over 23bp)
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAC71180.15842629582716533Illumina Paired End PCR Primer 2 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC70100.1560225251121704RNA PCR Primer, Index 41 (95% over 22bp)
AGTTCTACAGTCCGACGATCAAACGATCTCGTATGCCGTCTTCTGCTTGA68260.15192721204217904RNA PCR Primer, Index 28 (96% over 30bp)
GTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTT65440.14565069961969232RNA PCR Primer, Index 31 (96% over 27bp)
AGTTCTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCT65020.1447158998971943No Hit
AGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA64570.14371432876594642Illumina PCR Primer Index 7 (96% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCATCTCGTATGCCGTCTTCT61680.13728201638971TruSeq Adapter, Index 18 (96% over 29bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT61300.1364362452122118No Hit
GGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTTG61020.1358130453972131RNA PCR Primer, Index 34 (100% over 28bp)
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT60930.13561273117096354RNA PCR Primer, Index 24 (95% over 22bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCATCTCGTATGCCGTCTTC57880.12882430461472785RNA PCR Primer, Index 20 (100% over 21bp)
GGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA56980.1268211623522321Illumina PCR Primer Index 3 (96% over 28bp)
AGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGCTTG52340.11649385113225391RNA PCR Primer, Index 21 (96% over 28bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCATCTCGTATGCCGTCTTCT51660.1149803658672571TruSeq Adapter, Index 18 (100% over 24bp)
GTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC51120.11377848050975964TruSeq Adapter, Index 3 (96% over 25bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC49940.1111521384322652No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTATCTCGTATGCCGTCTTCTGC48050.10694553968102406RNA PCR Primer, Index 38 (100% over 26bp)
GGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCT47420.10554334009727703RNA PCR Primer, Index 31 (96% over 26bp)
AACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAA47270.10520948305352774TruSeq Adapter, Index 18 (100% over 27bp)
GTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA46530.10356245497103121Illumina PCR Primer Index 3 (96% over 29bp)
GCGTTGGTGGTATAGTGGTTAGCATAGCTGCCATCTCGTATGCCGTCTTC45900.10216025538728418RNA PCR Primer, Index 14 (95% over 22bp)
ACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA45860.10207122684228437RNA PCR Primer, Index 20 (96% over 30bp)
ACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA45690.10169285552603516TruSeq Adapter, Index 18 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)