FastQCFastQC Report
Thu 29 Jun 2023
SRR393007.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR393007.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15572852
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT6402304.111192991495713RNA PCR Primer, Index 31 (95% over 21bp)
AGTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAA3615622.321745560800295RNA PCR Primer, Index 28 (96% over 28bp)
GTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAA2279051.463476311211331Illumina PCR Primer Index 7 (96% over 27bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT2001521.2852623270291146TruSeq Adapter, Index 11 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT1551370.9962015949294323RNA PCR Primer, Index 41 (95% over 23bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC1527490.9808672168720284RNA PCR Primer, Index 20 (95% over 24bp)
TTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAA1230840.7903754559537328RNA PCR Primer, Index 28 (96% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC1148270.7373536973188983RNA PCR Primer, Index 14 (95% over 22bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG1099250.7058758408543278No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAATCTCGTATGCCGTCTTCT985430.6327871092591132RNA PCR Primer, Index 19 (95% over 24bp)
CGCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGC944370.6064207121470107TruSeq Adapter, Index 3 (96% over 26bp)
GAGTTCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGA850320.546027150325451RNA PCR Primer, Index 28 (96% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG783320.5030035602983962TruSeq Adapter, Index 18 (95% over 24bp)
GCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCT733020.4707037606213685RNA PCR Primer, Index 3 (96% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGGCTT597090.3834172443172259No Hit
TCTACAGTCCGACGATCAAACATCTCGTATGCCGTCTTCTGCTTGAAAAA570890.3665930941872433Illumina PCR Primer Index 7 (96% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTC550360.353409895631192TruSeq Adapter, Index 18 (95% over 22bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGATCTCGTATGCCGTCTTCTG549590.3529154454174483Illumina PCR Primer Index 10 (96% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCATCTCGTATGCCGTCTTCTG446230.2865435310115321TruSeq Adapter, Index 3 (96% over 26bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA428050.27486936882210145RNA PCR Primer, Index 31 (96% over 28bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT406160.2608128556028144No Hit
ACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTC395380.2538905526104017TruSeq Adapter, Index 3 (95% over 22bp)
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT394790.2535116881609098No Hit
GGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTTG392570.2520861304018044RNA PCR Primer, Index 34 (100% over 28bp)
CACCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTT384680.2470196210687676RNA PCR Primer, Index 3 (95% over 21bp)
AGTTCTACAGTCCGACGATCAAACGATCTCGTATGCCGTCTTCTGCTTGA380260.24418134841325145RNA PCR Primer, Index 28 (96% over 30bp)
CGCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCT370410.23785623853613969Illumina PCR Primer Index 3 (96% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCTCGTATGCCGTCTTCTG367630.23607108062158427Illumina PCR Primer Index 9 (96% over 25bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA349180.22422353978577592TruSeq Adapter, Index 3 (96% over 29bp)
GGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGAA346860.2227337677131973RNA PCR Primer, Index 41 (96% over 28bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCCATCTCGTATGCC345870.22209804600981245No Hit
CCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCT340940.2189322803555829RNA PCR Primer, Index 31 (95% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGATCTCGTATGCCGTCTTCTGC319480.20515188868423076Illumina PCR Primer Index 9 (96% over 25bp)
GGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTTGA307650.19755533540034928RNA PCR Primer, Index 38 (96% over 29bp)
ACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCG305920.19644442777726262No Hit
GTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTT299100.1920650115983893RNA PCR Primer, Index 24 (96% over 28bp)
GGCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTG297760.1912045397978482TruSeq Adapter, Index 18 (100% over 28bp)
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT274580.17631966193475673RNA PCR Primer, Index 20 (96% over 25bp)
TGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGC273580.17567751880002455RNA PCR Primer, Index 38 (100% over 24bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC265670.17059816660429317No Hit
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT258520.1660068431909582RNA PCR Primer, Index 24 (95% over 22bp)
GTTACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTC256180.16450422825568498RNA PCR Primer, Index 38 (100% over 22bp)
ACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGCT245290.15751128951845172RNA PCR Primer, Index 21 (96% over 26bp)
GTCCTCGGTTCGAAACCGAGCAGAAACACATCTCGTATGCCGTCTTCTGC242670.15582887450545346RNA PCR Primer, Index 39 (96% over 25bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC240760.15460238111811503RNA PCR Primer, Index 31 (95% over 22bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCGCATCTCGTATGCCG239380.15371622359218468No Hit
GTTCTACAGTCCGACGATCAAACGATCTCGTATGCCGTCTTCTGCTTGAA239140.15356210923984892RNA PCR Primer, Index 42 (96% over 29bp)
ACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGC238960.15344652347559715RNA PCR Primer, Index 38 (100% over 25bp)
GCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTGC238760.15331809484865072TruSeq Adapter, Index 3 (96% over 25bp)
GGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGA228540.14675539201168805RNA PCR Primer, Index 31 (96% over 28bp)
AGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCT228020.14642147758162732RNA PCR Primer, Index 34 (95% over 22bp)
CGCCTACCCGCCGCCGTCCGCCCCCGGATCTCGTATGCCGTCTTCTGCTT226630.14552889862434962RNA PCR Primer, Index 30 (96% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATCTCGTATGCCGTCTTC225090.14453999819686209RNA PCR Primer, Index 41 (95% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTC219460.14092473234832RNA PCR Primer, Index 31 (95% over 22bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACATCTCGTATGCCGT217860.13989730333274855No Hit
CTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTG216720.1391652601591539RNA PCR Primer, Index 33 (100% over 23bp)
CGCAGCAACCCCGGTTCGAATCCGGGTCACGGCATCTCGTATGCCGTCTT214870.1379772953598994RNA PCR Primer, Index 41 (95% over 22bp)
GCGCGCGGTCCCTCTCTCCCCCCGCATCTCGTATGCCGTCTTCTGCTTGA213250.1369370234816333TruSeq Adapter, Index 18 (96% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCATCTCGTATGCCGTCTTCT211940.13609581597513418TruSeq Adapter, Index 18 (96% over 29bp)
GCCTACCCGCCGCCGTCCGCCCCCGGCATCTCGTATGCCGTCTTCTGCTT210770.13534450850749752Illumina PCR Primer Index 3 (96% over 27bp)
AGTTCTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCT199940.12839009835834825No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAATCTCGTATGCCGTCTTCT197620.1269003262857696RNA PCR Primer, Index 40 (100% over 24bp)
TACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCTGC196540.12620681170025888RNA PCR Primer, Index 33 (100% over 24bp)
CGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCTG194960.12519222554738207RNA PCR Primer, Index 34 (95% over 24bp)
CTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCT194550.12492894686214188RNA PCR Primer, Index 38 (96% over 27bp)
TACTTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTG193180.12404921076755882RNA PCR Primer, Index 38 (96% over 25bp)
TCGCCTGCCGCGCGATGCCTCCGCTGGCCGGGGATCTCGTATGCCGTCTT190200.12213562422605699RNA PCR Primer, Index 23 (95% over 21bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAACCATCTCGTATGCCG186560.11979822321563191No Hit
TCCTCGTTAGTATAGTGGACAGTATCTCCGCATCTCGTATGCCGTCTTCT180350.11581051434894521TruSeq Adapter, Index 18 (100% over 24bp)
CGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTT179240.11509773546939252TruSeq Adapter, Index 3 (96% over 28bp)
CCGCCGGTCCCGGACCCCCAGGCCTCCCGGCATCTCGTATGCCGTCTTCT178390.11455191380487016TruSeq Adapter, Index 3 (95% over 23bp)
TCCTCGTTAGTATAGTGGACAGTATCTCCGCCATCTCGTATGCCGTCTTC177370.11389692780744336RNA PCR Primer, Index 20 (100% over 21bp)
GTTCAGAGTTCTACAGTCCACGATCAAACATCTCGTATGCCGTCTTCTGC176620.11341532045639423Illumina PCR Primer Index 6 (95% over 24bp)
GCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTC172560.11080821932938167RNA PCR Primer, Index 26 (95% over 22bp)
TTGGATAACTGTGGCAATTCCAGAGCTATCTCGTATGCCGTCTTCTGCTT169880.10908727572829949RNA PCR Primer, Index 38 (100% over 27bp)
GTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTT168150.10797636810521284RNA PCR Primer, Index 31 (96% over 27bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC165530.10629395309221458No Hit
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG164520.10564538852613509Illumina PCR Primer Index 3 (96% over 28bp)
GAGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTC164200.1054399027230208No Hit
AGTTCTACAGTCCGACGATCAAACGCCATCTCGTATGCCGTCTTCTGCTT162960.10464364523595292RNA PCR Primer, Index 41 (96% over 28bp)
TCTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTCT161920.10397581637583149RNA PCR Primer, Index 33 (100% over 22bp)
GCCTACCCGCCGCCGTCCGCCCCCGGATCTCGTATGCCGTCTTCTGCTTG160910.103327251809752RNA PCR Primer, Index 46 (96% over 29bp)
TTCTACAGTCCGACGATCAAACGATCTCGTATGCCGTCTTCTGCTTGAAA159850.10264658008693589RNA PCR Primer, Index 28 (96% over 30bp)
CTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTG157540.10116322944570462RNA PCR Primer, Index 38 (100% over 23bp)
GTTTCTGTAGTGTAGTGGTCATCACGTTCGCCTATCTCGTATGCCGTCTT157050.10084857930968585Illumina PCR Primer Index 10 (95% over 21bp)
TTCTACAGTCCGACGATCAAACGTACTCTGCGATCTCGTATGCCGTCTTC156930.10077152213351799RNA PCR Primer, Index 21 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)