FastQCFastQC Report
Mon 26 Jun 2023
SRR393008.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR393008.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067332
Total Bases220.8 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA674762.199827080994167RNA PCR Primer, Index 38 (100% over 27bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG333491.0872315093377567Illumina PCR Primer Index 3 (96% over 28bp)
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT249560.8136060915479643Illumina PCR Primer Index 3 (96% over 27bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT237560.7744841445269048TruSeq Adapter, Index 11 (95% over 24bp)
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA234680.7650948772418505RNA PCR Primer, Index 38 (100% over 27bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT233340.7607262598244989No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG232410.7576943089303668TruSeq Adapter, Index 18 (95% over 24bp)
GTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA213540.696175047239751Illumina PCR Primer Index 3 (96% over 28bp)
GAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAA177600.5790048159116783Illumina PCR Primer Index 3 (96% over 28bp)
GGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTTG162580.530037178890319RNA PCR Primer, Index 34 (100% over 28bp)
GTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC158700.5173877493535098TruSeq Adapter, Index 3 (96% over 25bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCT158390.5163770990554658Illumina PCR Primer Index 3 (96% over 26bp)
GGGGGATTAGCTCAGATGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA153050.49896783263109434RNA PCR Primer, Index 25 (96% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT149340.4868726306770836RNA PCR Primer, Index 31 (95% over 21bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC148830.4852099479286885Illumina PCR Primer Index 3 (96% over 25bp)
GGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA113760.37087605775964255RNA PCR Primer, Index 31 (96% over 28bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA110570.36047614017654434RNA PCR Primer, Index 31 (96% over 28bp)
ATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGA105820.3449903694807083RNA PCR Primer, Index 30 (96% over 29bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC100230.3267660624933982Illumina PCR Primer Index 3 (95% over 22bp)
GTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA100030.3261140300430472Illumina PCR Primer Index 3 (96% over 29bp)
GGGGAATTAGCTCAAGTGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA99210.32344069699660816TruSeq Adapter, Index 25 (96% over 28bp)
GTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC98090.3197893152746426TruSeq Adapter, Index 3 (96% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT97790.31881126659911607RNA PCR Primer, Index 41 (95% over 23bp)
AGTTCTACAGTCCGACGATCGGGCATCTCGTATGCCGTCTTCTGCTTGAA96660.315127283254633Illumina PCR Primer Index 3 (96% over 28bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGATCTCGTATGCCGTCTTCTG93690.3054446013669208RNA PCR Primer, Index 23 (95% over 24bp)
GTACTCTGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA93640.3052815932543331RNA PCR Primer, Index 21 (96% over 28bp)
GTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA93280.3041079348437013RNA PCR Primer, Index 30 (100% over 28bp)
AATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTG89450.2916215134194799RNA PCR Primer, Index 30 (100% over 28bp)
GGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTTGA88900.2898284241810146TruSeq Adapter, Index 3 (96% over 29bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCT88570.28875257063793547Illumina PCR Primer Index 3 (95% over 24bp)
GGCGACCCTGGGCGCGCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA82100.267659320869081RNA PCR Primer, Index 20 (100% over 27bp)
AAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTG81660.2662248494783088Illumina PCR Primer Index 3 (95% over 24bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC81060.26426875212725587No Hit
GATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA79150.2580418422264039TruSeq Adapter, Index 18 (100% over 27bp)
GAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGAAAAA77310.2520431436831748RNA PCR Primer, Index 30 (100% over 28bp)
TGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA76680.2499892414645692Illumina PCR Primer Index 3 (96% over 28bp)
TAACTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGC73570.23985013686161133No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCT73300.2389698930536375TruSeq Adapter, Index 18 (95% over 23bp)
AGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA72860.23753542166286531RNA PCR Primer, Index 31 (96% over 28bp)
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT72740.2371442021926547No Hit
GGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGAA70470.229743633881171RNA PCR Primer, Index 41 (96% over 28bp)
TTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGA69070.2251794067287141Illumina PCR Primer Index 3 (96% over 28bp)
TGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGC69050.225114203483679RNA PCR Primer, Index 38 (100% over 24bp)
GTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGCTTGA67910.22139761851667833TruSeq Adapter, Index 11 (96% over 29bp)
AGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAA67350.21957192765569558Illumina PCR Primer Index 3 (96% over 28bp)
GGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGA66570.2170290010993267RNA PCR Primer, Index 31 (96% over 28bp)
GTTGGTGGTTCAAGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC64310.20966103441036055Illumina PCR Primer Index 3 (96% over 25bp)
GTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGC64150.20913940845007972TruSeq Adapter, Index 18 (96% over 25bp)
GACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGC62700.20441217318503507RNA PCR Primer, Index 18 (96% over 25bp)
ACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCG60610.19759843407886726No Hit
GCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCT59320.19339282477410336RNA PCR Primer, Index 3 (96% over 27bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTT55930.18234087474065408RNA PCR Primer, Index 30 (96% over 28bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCT54250.17686380215770578RNA PCR Primer, Index 30 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC54060.17624437132987236No Hit
GTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTT53970.1759509567272144RNA PCR Primer, Index 24 (96% over 28bp)
TAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAA50840.16574664887922141RNA PCR Primer, Index 38 (100% over 27bp)
CGGAGGAGGGGGGGTGGAGAGGCATCTCGTATGCCGTCTTCTGCTTGAAA49870.16258429149501913RNA PCR Primer, Index 3 (96% over 29bp)
GGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTG47320.15427087775304402TruSeq Adapter, Index 18 (95% over 24bp)
AGTTCTACAGTCCGACGATCGGGCGTACTCTGCGATCTCGTATGCCGTCT47070.15345583719010528No Hit
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT46680.15218437391192086RNA PCR Primer, Index 24 (95% over 22bp)
GTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAA45910.14967404897806955TruSeq Adapter, Index 11 (96% over 29bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC42390.13819827785189212RNA PCR Primer, Index 14 (95% over 22bp)
GGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTTGAA41340.13477510748754945RNA PCR Primer, Index 3 (96% over 29bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC40180.1309933192755137RNA PCR Primer, Index 20 (95% over 24bp)
GGGGGGCGGGATGCGCAGCGGTTGGCGGCATCTCGTATGCCGTCTTCTGC39550.1289394170569081TruSeq Adapter, Index 3 (96% over 25bp)
GCGTTGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTG38520.1255814499376005TruSeq Adapter, Index 18 (95% over 24bp)
AACTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCC38280.1247990109971793No Hit
GTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA37710.12294071851367899TruSeq Adapter, Index 18 (96% over 28bp)
GGGGAATTAGCTCAGATGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA37670.12281031202360879RNA PCR Primer, Index 25 (96% over 28bp)
CGCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGC36990.12059340169241542TruSeq Adapter, Index 3 (96% over 26bp)
AGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCT36460.1188655156989853RNA PCR Primer, Index 34 (95% over 22bp)
ATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA36180.11795267026849393TruSeq Adapter, Index 11 (96% over 29bp)
GGGGAATTAGCTCAAATGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA35870.11694201997044988TruSeq Adapter, Index 25 (96% over 28bp)
GGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA35810.1167464102353446Illumina PCR Primer Index 3 (96% over 28bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCT35280.11501852424191446RNA PCR Primer, Index 30 (95% over 24bp)
GGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGCTTGAAA34740.1132580366259668TruSeq Adapter, Index 11 (96% over 29bp)
GGGGGCGGGATGCGCAGCGGTTGGCGGCATCTCGTATGCCGTCTTCTGCT34200.11149754901001914TruSeq Adapter, Index 3 (96% over 26bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA34170.11139974414246649TruSeq Adapter, Index 3 (96% over 29bp)
TGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTC34020.11091071980470324No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG33910.1105521019570102No Hit
CGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTT33790.1101608824867996TruSeq Adapter, Index 3 (96% over 28bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT33690.1098348662616241No Hit
GTTGTGGGTTCGTGTCCCACCAGAGTCGCATCTCGTATGCCGTCTTCTGC33540.10934584192386088RNA PCR Primer, Index 24 (96% over 26bp)
GGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGCTTG33420.10895462245365027TruSeq Adapter, Index 18 (96% over 28bp)
TAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAA32840.10706372834763239Illumina PCR Primer Index 3 (96% over 28bp)
GTGGCGCAGCGGAAGCGTGCTGGGATCTCGTATGCCGTCTTCTGCTTGAA32310.10533584235420228RNA PCR Primer, Index 34 (96% over 28bp)
GGGGATGTAGCTCAGTGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA32130.10474901314888639RNA PCR Primer, Index 34 (96% over 28bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGATCTCGTATGCCGTCTTCTG32130.10474901314888639Illumina PCR Primer Index 3 (95% over 23bp)
GTGAGGTCCTCGGATCGGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA31110.10142364765209635RNA PCR Primer, Index 16 (96% over 29bp)
TAACTGAAAGGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGC30800.10041299735405232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)