FastQCFastQC Report
Mon 26 Jun 2023
SRR393009.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR393009.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14143962
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA2936822.0763771848368937RNA PCR Primer, Index 38 (100% over 27bp)
ATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTG1424731.007306156506925Illumina PCR Primer Index 3 (96% over 28bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCT1195720.8453925427684266No Hit
AATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTT1061930.7508009424799077Illumina PCR Primer Index 3 (96% over 27bp)
GTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1041780.7365545806754854RNA PCR Primer, Index 38 (100% over 27bp)
GTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA1039260.73477290168059Illumina PCR Primer Index 3 (96% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCT1023740.723800021521551TruSeq Adapter, Index 11 (95% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCATCTCGTATGCCGTCTTCTG939830.6644743530843762TruSeq Adapter, Index 18 (95% over 24bp)
GGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTTG917120.6484180316660919RNA PCR Primer, Index 34 (100% over 28bp)
GTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC906760.6410933513537437TruSeq Adapter, Index 3 (96% over 25bp)
GAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAAA824020.5825948910213419Illumina PCR Primer Index 3 (96% over 28bp)
GGGGGATTAGCTCAGATGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA725410.5128760951139433RNA PCR Primer, Index 25 (96% over 28bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCT654450.4627062770672037Illumina PCR Primer Index 3 (96% over 26bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGC611930.43264397910571306Illumina PCR Primer Index 3 (96% over 25bp)
GGCGACCCTGGGCGCGCGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA581140.41087497265617656RNA PCR Primer, Index 20 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTT550600.3892827200751812RNA PCR Primer, Index 31 (95% over 21bp)
GTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC549850.3887524584695575TruSeq Adapter, Index 3 (96% over 25bp)
GTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCTGCTTGA543330.38414271757800256Illumina PCR Primer Index 3 (96% over 29bp)
GGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA516090.3648836160617513RNA PCR Primer, Index 31 (96% over 28bp)
GGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTTGA463200.327489567633171TruSeq Adapter, Index 3 (96% over 29bp)
GGGGAATTAGCTCAAGTGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA459150.3246261549628032TruSeq Adapter, Index 25 (96% over 28bp)
TGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA456810.3229717387532574Illumina PCR Primer Index 3 (96% over 28bp)
AGTTCTACAGTCCGACGATCGGGCATCTCGTATGCCGTCTTCTGCTTGAA416220.294273980656905Illumina PCR Primer Index 3 (96% over 28bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGATCTCGTATGCCGTCTTCTG414190.2928387392443504RNA PCR Primer, Index 23 (95% over 24bp)
ATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGA407380.28802396386528756RNA PCR Primer, Index 30 (96% over 29bp)
GATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA404490.28598068914495106TruSeq Adapter, Index 18 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATCTCGTATGCCGTCTTCT401720.28402225628151434RNA PCR Primer, Index 41 (95% over 23bp)
GGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGAA399770.28264357610689284RNA PCR Primer, Index 41 (96% over 28bp)
GTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA396450.28029628473266543RNA PCR Primer, Index 30 (100% over 28bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCATCTCGTATGCCGTCTTCT395810.2798437948291999Illumina PCR Primer Index 3 (95% over 24bp)
GTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCT387130.27370690051344876TruSeq Adapter, Index 18 (95% over 23bp)
GAAAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTC383670.2712606269728383Illumina PCR Primer Index 3 (95% over 22bp)
GTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAA382050.2701152619046912RNA PCR Primer, Index 31 (96% over 28bp)
CGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCGT376540.26621960664204275No Hit
TAACTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGC375100.26520150435924533No Hit
AATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTG354000.25028347785436644RNA PCR Primer, Index 30 (100% over 28bp)
GCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCT343960.24318504249375103RNA PCR Primer, Index 3 (96% over 27bp)
GTTGGTGGTTCAAGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGC341940.241756871235938Illumina PCR Primer Index 3 (96% over 25bp)
AAAAATTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTG333910.236079536978394Illumina PCR Primer Index 3 (95% over 24bp)
GAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTTGAAAAA331050.23405747272228247RNA PCR Primer, Index 30 (100% over 28bp)
AGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAA317910.22476728939175597RNA PCR Primer, Index 31 (96% over 28bp)
GACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTCTTCTGC311020.21989595277475998RNA PCR Primer, Index 18 (96% over 25bp)
TTAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGA291180.20586876576732882Illumina PCR Primer Index 3 (96% over 28bp)
AGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAAA289200.2044688751284824Illumina PCR Primer Index 3 (96% over 28bp)
ACGCGGAAGACCGGGGTTCGATTCCCCGACGGGGAGCATCTCGTATGCCG286570.20260942443142876No Hit
GTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGCTTGA283460.200410606306776TruSeq Adapter, Index 11 (96% over 29bp)
GTACTCTGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA282490.1997248012968361RNA PCR Primer, Index 21 (96% over 28bp)
TGCGCAGCGGTTGGCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTC280670.198438033133856No Hit
GGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTG271520.19196884154524735TruSeq Adapter, Index 18 (95% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCGTC267960.1894518664572204No Hit
TGGTGGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGC256300.18120806602845793RNA PCR Primer, Index 38 (100% over 24bp)
GTTGGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGC255050.18032429668575184TruSeq Adapter, Index 18 (96% over 25bp)
GTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTTCTGCTT250090.17681749993389406RNA PCR Primer, Index 24 (96% over 28bp)
GAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCTT232270.16421848418427595RNA PCR Primer, Index 24 (95% over 22bp)
GGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA231740.16384376598296857Illumina PCR Primer Index 3 (96% over 28bp)
CGGAGGAGGGGGGGTGGAGAGGCATCTCGTATGCCGTCTTCTGCTTGAAA231460.1636458016502024RNA PCR Primer, Index 3 (96% over 29bp)
GGTCTAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGA225580.15948855066211293RNA PCR Primer, Index 31 (96% over 28bp)
CGCGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGC221520.15661806783700352TruSeq Adapter, Index 3 (96% over 26bp)
CATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTC217850.15402332104681843RNA PCR Primer, Index 20 (95% over 24bp)
AAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCTT217590.15383949702353555RNA PCR Primer, Index 30 (96% over 28bp)
CGGGGGAGTGGCGCGCGGCCTCGGCATCTCGTATGCCGTCTTCTGCTTGA217560.1538182865593106TruSeq Adapter, Index 3 (96% over 29bp)
AGTTCTACAGTCCGACGATCGGGCGTACTCTGCGATCTCGTATGCCGTCT213890.1512235397691255No Hit
GGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA213520.1509619440436845TruSeq Adapter, Index 18 (96% over 28bp)
AAAATTAGAGTGTTCAAGGCAGGGCCGGATCTCGTATGCCGTCTTCTGCT208560.1474551472918267RNA PCR Primer, Index 30 (100% over 26bp)
GTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAA202910.14346050986279515TruSeq Adapter, Index 11 (96% over 29bp)
CGGGGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTT196340.13881541819753193TruSeq Adapter, Index 3 (96% over 28bp)
TAGTGGTTAGGATTCGGCGCTATCTCGTATGCCGTCTTCTGCTTGAAAAA195600.13829222674664993RNA PCR Primer, Index 38 (100% over 27bp)
GGGGCGGGATGCGCAGCGGTTGGCATCTCGTATGCCGTCTTCTGCTTGAA192760.13628430280002166RNA PCR Primer, Index 3 (96% over 29bp)
AACTGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCC183630.12982925152089633No Hit
GTTGTGGGTTCGTGTCCCACCAGAGTCGCATCTCGTATGCCGTCTTCTGC182690.12916465697518134RNA PCR Primer, Index 24 (96% over 26bp)
GGGGAATTAGCTCAGATGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA182060.12871923722645748RNA PCR Primer, Index 25 (96% over 28bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCATCTCGTATGCCGTCTTC179940.12722036442122794RNA PCR Primer, Index 14 (95% over 22bp)
TGAAAGGTTGGTGGTTCAGGCCCACCCAGGGACGCATCTCGTATGCCGTC179550.12694462838630363No Hit
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCCATCTCGTATGCCGT172700.12210157238827424No Hit
AGAGGCCGTGGGGAGGAAAGGGGTGGTTCGTGGCATCTCGTATGCCGTCT169500.11983912287094664RNA PCR Primer, Index 34 (95% over 22bp)
GTGGCGCAGCGGAAGCGTGCTGGGATCTCGTATGCCGTCTTCTGCTTGAA168180.11890586244504898RNA PCR Primer, Index 34 (96% over 28bp)
GTGGTTAGGATTCGGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA166420.1176615152105188TruSeq Adapter, Index 18 (96% over 28bp)
GCGTTGTGGTATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTG163790.11580206451346517TruSeq Adapter, Index 18 (95% over 24bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCGCATCTCGTATGCCG162460.11486173393282588No Hit
GGGGATGTAGCTCAGTGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAA158210.11185691816762518RNA PCR Primer, Index 34 (96% over 28bp)
TGCCGAGGGCGAGGGACCTGTGGCGTACGGAGGGCATCTCGTATGCCGTC157760.11153876120425098No Hit
TAACTGAAAGGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGC155140.109686380661939No Hit
ATCCCGGGGCCACGCCTGTCTGAGGGTCGCCATCTCGTATGCCGTCTTCT155130.10967931050719734RNA PCR Primer, Index 20 (96% over 25bp)
GCGGCGGCGACCCTGGGCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAA154150.10898643534251577RNA PCR Primer, Index 26 (96% over 28bp)
GTGAGGTCCTCGGATCGGCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA153180.10830063033257585RNA PCR Primer, Index 16 (96% over 29bp)
GGGGAATTAGCTCAAATGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAA153050.1082087183209344TruSeq Adapter, Index 25 (96% over 28bp)
ATAGTGGTGAGCATAGCTGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA148450.10495644713977596TruSeq Adapter, Index 11 (96% over 29bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGATCTCGTATGCCGTCTTCTG144150.101916280600867Illumina PCR Primer Index 3 (95% over 23bp)
GGTTGGTGGTTCATGCCCACCCAGGGACGCATCTCGTATGCCGTCTTCTG143910.10174659688706741TruSeq Adapter, Index 18 (95% over 24bp)
TAGAGTGTTCAAGGCAGGGCCGGCATCTCGTATGCCGTCTTCTGCTTGAA142630.10084161708013638Illumina PCR Primer Index 3 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)