FastQCFastQC Report
Sun 2 Jul 2023
SRR3945920.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3945920.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13107868
Total Bases655.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG7961226.073619294915084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA2134111.6281137405411772Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATCTTGTAGAGAACTTTTACTCTGAATAGATCGGAAGAGCACACGTCT1459061.113117709149955Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTC1149500.8769542079612033No Hit
ATATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGTC1092220.8332552631747588No Hit
TATATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGT1037650.7916237789394889No Hit
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACCTCTGA805290.6143562019391712Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGTTACGATCTGCTGAGATTAAGCCTTTGTAGATCGGAAGAGCACACGTC615830.4698170594943434No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGT575240.43885092526107217No Hit
TACGACTCCACTCACGGTAGATACGCTGGAGATCGGAAGAGCACACGTCT561930.428696718642574Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCAGATCGGAAGAGCACACGTCT530150.4044517384520503Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTTGGGTTTTGGACAAGTTAAAGGCTGAGATCGGAAGAGCACACGTCT494020.37688814077163424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTCCGTCTAGCTCCAGAATTGAACCAAGCTAGATCGGAAGAGCACACGT433850.33098441333098566No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTAGATCGGAAGAGCACACGTCT409820.31265191257647695Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTTACGATCTGCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGT395680.3018644984828959No Hit
AATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC326050.24874373162744695Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTAGAAGACTTAAGAGAAAGAGACGGAAGATCGGAAGAGCACACGTCT324300.24740865562576614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATCACCGTCTACAAGGCTTTGAAGTCTGCTAGATCGGAAGAGCACACGT315940.24103080684059375No Hit
TATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGTCT306540.23385954146013677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACGTGCTGCTTTGACTGACTTTGAAAGAGATCGGAAGAGCACACGTC298460.2276953048352333No Hit
TGTCCAAGATTTGGACTTGAAGGACAAGCGTGAGATCGGAAGAGCACACG293780.22412493015645257No Hit
GTCCGTCTAGCTCCAGAATTGAACCAAGCTAGATCGGAAGAGCACACGTC293760.22410967214500482No Hit
GATCTTGTAGAGAACTTTTACTCTGAATAGATCGGAAGAGCACACGTCTG284550.21708335787330174Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACATCTGA252740.19281549066560635Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GAGACGTTATGACCGTAAACAATCTGGTTTAGATCGGAAGAGCACACGTC222030.16938681408753886No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTC221600.16905876684141158No Hit
AAGACCGACGGTAACTCCTTCTTGATCAACTAGATCGGAAGAGCACACGT217440.16588510046027316No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTAGATCGGAAGAGCACACGTCT217020.16556468221986978Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTAGAAGACTTAAGAGAAAGAGACGGAGATCGGAAGAGCACACGTCTG213200.16265040203334363Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGGTGCTCAAGGTACAAAATTCAGAATTAGATCGGAAGAGCACACGTC203730.15542573361281942No Hit
ATCACCGTCTACAAGGCTTTGAAGTCTGCTAGATCGGAAGAGCACACGTC200180.15271743658083833No Hit
AACGGTTTCGGTAGAATCGGTAGATCGGAAGAGCACACGTCTGAACTCCA192900.1471635204138461Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCCACTCACGGTAGATACGCTGGTGAAGATCGGAAGAGCACACGTC191380.14600391154381476No Hit
TGAAAGACGTGCTGCTTTGACTGACTTTGAAAGATCGGAAGAGCACACGT191210.1458742184465086No Hit
ACTTCCGTCTTGGATTTCGTTTTCGAATCTGAGATCGGAAGAGCACACGT189320.14443233636469333No Hit
TTCGGTGCTCTAAAGGGTGCTTCTGACGGTAGATCGGAAGAGCACACGTC186760.14247931089937738No Hit
CGTGCTGTCTTCCCATCTATCGTCGGTAGAGATCGGAAGAGCACACGTCT178170.13592599498255553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGTCTGAACTCC177390.13533093253609207Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGATGTCGGCTCTTCCTATCATACCGAAGAGATCGGAAGAGCACACGT172220.13138673657684072No Hit
AACGCTATCTTGGATGGTGCTGACTGTGTAGATCGGAAGAGCACACGTCT171740.13102054430209398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC166900.12732810553173102Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATGATTAATAGGGACGGTCGGGGGCATCAAGATCGGAAGAGCACACGTC164160.12523775796338504No Hit
GGGGCTGATCCGGGTTGAAGACATTGTCAAGATCGGAAGAGCACACGTCT163410.12466558253409327Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGTGCTCGTTCTAGATTCCAAAAATCTAGATCGGAAGAGCACACGTC161360.12310163636069572No Hit
AACGCTATCTTGGATGGTGCTGACTGTGTTAGATCGGAAGAGCACACGTC156960.11974487384218396No Hit
TATCACCGTCTACAAGGCTTTGAAGTCTGCAGATCGGAAGAGCACACGTC153470.11708235084454618No Hit
CGTGCTGCTTTGACTGACTTTGAAAGATTCAGATCGGAAGAGCACACGTC151450.11554129168832032No Hit
TGAAAGACGTGCTGCTTTGACTGACTTTGAAGATCGGAAGAGCACACGTC149420.11399260352637057No Hit
AAAGACCGACGGTAACTCCTTCTTGATCAACTAGATCGGAAGAGCACACG149300.11390105545768389No Hit
AGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTCTGAACT144000.10985768242402197Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTTCCGTCTTGGATTTCGTTTTCGAATCTGAGATCGGAAGAGCACACG136930.10446397537723145No Hit
AAGACGTGCTGCTTTGACTGACTTTGAAAGAAGATCGGAAGAGCACACGT136920.10445634637150755No Hit
AGACGTGCTGCTTTGACTGACTTTGAAAGAGATCGGAAGAGCACACGTCT136060.10380025187925299Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGGTGCTTCTGCCGGTAACGTCGGTGAAGATCGGAAGAGCACACGTCT135570.10342643059878234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGCTGACTGTGCTATCTTGATTATTGCTAGATCGGAAGAGCACACGTC133180.10160309823077254No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCT132310.10093937473279407Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTGTGGTAATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGT131870.1006036984809429No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)