FastQCFastQC Report
Sun 2 Jul 2023
SRR3945921.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3945921.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25665535
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTTCCGTCTTGGATTTCGTTTTCGAATCTGAGATCGGAAGAGCACACG7398162.88252709324002No Hit
TATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGTCT5017831.9550849027694144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATCTTGTAGAGAACTTTTACTCTGAATAGATCGGAAGAGCACACGTCT4880441.9015539711134017Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGTC3853821.5015545165919977No Hit
TGTCCGTCTAGCTCCAGAATTGAACCAAGCTAGATCGGAAGAGCACACGT3473611.3534142187178253No Hit
TATATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGT3111661.212388520247094No Hit
TGGGCTGCTAGAGCTGCTTCTGCCAAGATCGGAAGAGCACACGTCTGAAC2865151.1163414283006374Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGACGTGCTGCTTTGACTGACTTTGAAAGAAGATCGGAAGAGCACACGT2376920.9261135604615294No Hit
ATTGCTCTAACTGATCTTGACTCTCCATCAAGATCGGAAGAGCACACGTC2136310.8323652711700731No Hit
AAGACGTGCTGCTTTGACTGACTTTGAAAGAGATCGGAAGAGCACACGTC1960270.763775233985966No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGT1812400.7061610054105633No Hit
CATTGCTCTAACTGATCTTGACTCTCCATCAAGATCGGAAGAGCACACGT1652430.6438322832545669No Hit
GTCCGTCTAGCTCCAGAATTGAACCAAGCTAGATCGGAAGAGCACACGTC1576420.614216691761929No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTC1566410.6103165197998015No Hit
ACTTCCGTCTTGGATTTCGTTTTCGAATCTGAGATCGGAAGAGCACACGT1526130.5946223213348173No Hit
CTTCAAGATCTCTGGTGGTAACGACAAACAAGAGATCGGAAGAGCACACG1320300.5144252788808026No Hit
AACGGTTTCGGTAGAATCGGTAGATCGGAAGAGCACACGTCTGAACTCCA1169160.4555369681559336Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACTAGAAGACTTAAGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCC1156880.4507523416129841Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACG1146820.4468326882724245No Hit
GCCGGTGTCGTCAAGGGTAAGACTTTGTTGAGATCGGAAGAGCACACGTC979640.38169475134650416No Hit
TACGACTCCACTCACGGTAGATACGCTGGAGATCGGAAGAGCACACGTCT978090.3810908286151058Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGCTGCTTTGACTGACTTTGAAAGATTCAGATCGGAAGAGCACACGTC953540.3715254718048932No Hit
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG922830.35956000917183295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTGCTGCTTTGACTGACTTTGAAAGATAGATCGGAAGAGCACACGT901370.3511986015487306No Hit
ACTAGAAGACTTAAGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCCA886970.3455879645602556Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAAAGACGTGCTGCTTTGACTGACTTTGAAAAGATCGGAAGAGCACACGT777810.3030562191670659No Hit
CTTCCGTCTTGGATTTCGTTTTCGAATCTGAGATCGGAAGAGCACACGTC735160.2864386033643951No Hit
TAGAAGACTTAAGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGT713170.2778706931299114Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGTGCTGCTTTGACTGACTTTGAAAGAGATCGGAAGAGCACACGTCT651180.2537176801496637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTGCTGCTTTGACTGACTTTGAAAGATTAGATCGGAAGAGCACACGT648140.2525332123409857No Hit
CTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTCT623800.24304967732018834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGACTGTGCTTCCTCTGAATTCTTCAAGGAAGATCGGAAGAGCACACGT604000.2353350514610352No Hit
ATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGTCTG533310.2077922786335839Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGACGTTATGACCGTAAACAATCTGGTTTAGATCGGAAGAGCACACGTC532670.20754291698965166No Hit
CTCTTCCGTCTCTGCTACTTCTACTGCTAGTTAGATCGGAAGAGCACACG528100.20576231900094813No Hit
TTCGAACTGTTGAACTTGACCTCCAGATCGGAAGAGCACACGTCTGAACT522640.2036349524761514Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGCTGTCTTCCCATCTATCGTCGGTAGAGATCGGAAGAGCACACGTCT515680.20092314459838845Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA503840.19630995418564232Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGCTGCTTTGACTGACTTTGAAAGAAGATCGGAAGAGCACACGTC499960.19479819921930325No Hit
TTGGACTGTGCTTCCTCTGAATTCTTCAAGGAGATCGGAAGAGCACACGT499910.19477871784087103No Hit
TGTTACGATCTGCTGAGATTAAGCCTTTGTAGATCGGAAGAGCACACGTC493520.19228899767723526No Hit
CAGATCTGGTGAAACTGAAGACACTTTCATTAGATCGGAAGAGCACACGT466110.18160930602070052No Hit
ACGTGCTGCTTTGACTGACTTTGAAAGATTCAGATCGGAAGAGCACACGT444170.17306087716464902No Hit
ATTGTGTGACTACTCCGACTACATGGCTGTAGATCGGAAGAGCACACGTC431110.1679723411181571No Hit
TTGTGTGACTACTCCGACTACATGGCTGTAGATCGGAAGAGCACACGTCT373360.14547134902896044Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTCT371900.14490249277874004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACGTGCTGCTTTGACTGACTTTGAAAAGATCGGAAGAGCACACGTCT371500.1447466417512824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTATCTTCCGGCTCGGGCTATAAATACTCGAGATCGGAAGAGCACACGTC359930.1402386507820702No Hit
TATCACCGTCTACAAGGCTTTGAAGTCTGCTAGATCGGAAGAGCACACGT354090.13796322578118866No Hit
GGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTCTG352560.1373670956011632Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTTGCTATTAACGGTTTCGGTAGAATCGAGATCGGAAGAGCACACGT346670.13507218922184946No Hit
CAAGAAGGTTTGGATGCTTTGGACGACGTGAGATCGGAAGAGCACACGTC344420.13419552719240024No Hit
GGGGCTGATCCGGGTTGAAGACATTGTCAAGATCGGAAGAGCACACGTCT343910.13399681713239175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGACTTCACTACCGACAAATTGAGATCGGAAGAGCACACGTCTGAACT336410.13107461036756102Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTC333290.12985897235339142No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTAGATCGGAAGAGCACACGTCT313490.12214434649423828Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAGACGTGCTGCTTTGACTGACTTTGAAAGATCGGAAGAGCACACGT302590.11789740599601761No Hit
GAACTAGAAGACTTAAGAGAAAGAGATCGGAAGAGCACACGTCTGAACTC295800.1152518348049242Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACGTGCGATGACGACTATACTGGACAAGGTAGATCGGAAGAGCACACGT288050.11223222114793244No Hit
TGAAAGACGTGCTGCTTTGACTGACTTTGAAAAGATCGGAAGAGCACACG282010.10987887063332206No Hit
CGCTTGGGTTTTGGACAAGTTAAAGGCTGAGATCGGAAGAGCACACGTCT273090.10640339272101672Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAACGGTTTCGGTAGAATCGGTAGATCGGAAGAGCACACGTCTGAACT269280.10491891168448271Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTTTGGACTGTGCTTCCTCTGAATTCTTCAGATCGGAAGAGCACACGT268890.10476695693271151No Hit
ATGTCCGTCTAGCTCCAGAATTGAACCAAGCTAGATCGGAAGAGCACACG267360.10417082675268603No Hit
ACGTGCTGCTTTGACTGACTTTGAAAGATTAGATCGGAAGAGCACACGTC267330.10415913792562671No Hit
CCCGGTCTGAAAGATTATGAAGTGTCTTTTAGATCGGAAGAGCACACGTC267300.1041474490985674No Hit
TTCGATGTCGGCTCTTCCTATCATACCGAAGAGATCGGAAGAGCACACGT264590.1030915583875419No Hit
TCAGACGTTGTGCTCGGTGTCCTGGCCACTAGATCGGAAGAGCACACGTC263760.10276816750556729No Hit
ATATATTAGCATGGAATAATAGAATAGGACGAGATCGGAAGAGCACACGT263420.10263569413222828No Hit
AAGGGTGCTCGTTCTAGATTCCAAAAATCTAGATCGGAAGAGCACACGTC261000.10169279541610958No Hit
TGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTCTG259350.10104990992784682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGTTGGAAAAGAACGACAGAAGATCGGAAGAGCACACGTCTGAACTCC256780.10004856707643149Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)