FastQCFastQC Report
Sun 2 Jul 2023
SRR3945922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3945922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26443593
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTTCCGTCTTGGATTTCGTTTTCGAATCTGAGATCGGAAGAGCACACG6333732.3951851021152835No Hit
CGATCTTGTAGAGAACTTTTACTCTGAATAGATCGGAAGAGCACACGTCT4602881.7406409181989755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGTC3910571.4788345895355446No Hit
TAGAAGACTTAAGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGT2444600.9244583366564446Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGACGTGCTGCTTTGACTGACTTTGAAAGAAGATCGGAAGAGCACACGT2404230.9091918787284315No Hit
AACTAGAAGACTTAAGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCC2378130.8993218130380392Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGACGTGCTGCTTTGACTGACTTTGAAAGAGATCGGAAGAGCACACGTC2337920.8841158612598522No Hit
ACTAGAAGACTTAAGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCCA2226820.8421019034743124Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCCGTCTAGCTCCAGAATTGAACCAAGCTAGATCGGAAGAGCACACGT2138040.8085285535895217No Hit
ACTTCCGTCTTGGATTTCGTTTTCGAATCTGAGATCGGAAGAGCACACGT2092390.7912653927172454No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTC2006090.7586298881547602No Hit
CTTCAAGATCTCTGGTGGTAACGACAAACAAGAGATCGGAAGAGCACACG1909890.7222505655717814No Hit
TATATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGT1773030.6704951176642296No Hit
AGAAGACTTAAGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1733610.6555879150008094Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGCTGCTAGAGCTGCTTCTGCCAAGATCGGAAGAGCACACGTCTGAAC1539690.5822544614115034Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGCTCTAACTGATCTTGACTCTCCATCAAGATCGGAAGAGCACACGTC1514060.5725621325362253No Hit
TATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGTCT1393810.5270879793075018Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGTTTCGGTAGAATCGGTAGATCGGAAGAGCACACGTCTGAACTCCA1361770.5149716228048132Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTCTG1274100.48181803433444165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACG1197230.45274861097733576No Hit
GACGTGCTGCTTTGACTGACTTTGAAAGATTAGATCGGAAGAGCACACGT1140430.4312689277890489No Hit
GTCCGTCTAGCTCCAGAATTGAACCAAGCTAGATCGGAAGAGCACACGTC1139580.4309474888680975No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGT1129950.4273057749754354No Hit
GAAAGACGTGCTGCTTTGACTGACTTTGAAAAGATCGGAAGAGCACACGT1124040.42507082906623167No Hit
CAAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTG984080.37214307450579803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAGACGTGCTGCTTTGACTGACTTTGAAAAGATCGGAAGAGCACACG973930.36830471562620104No Hit
GGGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTCT947800.35842330503271624Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGACTCCACTCACGGTAGATACGCTGGAGATCGGAAGAGCACACGTCT939200.35517109947956016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC910790.3444274762510526TruSeq Adapter, Index 9 (100% over 50bp)
AGACGTGCTGCTTTGACTGACTTTGAAAGATAGATCGGAAGAGCACACGT859250.3249369327383007No Hit
AGACGTGCTGCTTTGACTGACTTTGAAAGAGATCGGAAGAGCACACGTCT832280.31473786485822863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCCGTCTTGGATTTCGTTTTCGAATCTGAGATCGGAAGAGCACACGTC744950.2817128519562376No Hit
GCCGGTGTCGTCAAGGGTAAGACTTTGTTGAGATCGGAAGAGCACACGTC716740.27104486141501266No Hit
CGTGCTGCTTTGACTGACTTTGAAAGATTCAGATCGGAAGAGCACACGTC707390.26750903328454645No Hit
TGGACTGTGCTTCCTCTGAATTCTTCAAGGAAGATCGGAAGAGCACACGT673390.25465147644648745No Hit
AAGACGTGCTGCTTTGACTGACTTTGAAAAGATCGGAAGAGCACACGTCT607530.22974563252429425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTGCTGCTTTGACTGACTTTGAAAGAAGATCGGAAGAGCACACGTC605590.22901199545765208No Hit
CGGGCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTC584950.2212067021300774No Hit
ATTGTGTGACTACTCCGACTACATGGCTGTAGATCGGAAGAGCACACGTC542880.20529736636016144No Hit
CGGTGCTGCTGGTGGTCTAGGTTCTTTGGCTAGATCGGAAGAGCACACGT507140.1917818051427429No Hit
TTCGAACTGTTGAACTTGACCTCCAGATCGGAAGAGCACACGTCTGAACT494040.18682786412572602Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGTTGGAAAAGAACGACAGAAGATCGGAAGAGCACACGTCTGAACTCC491090.1857122819883062Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGAAGGTTTGGATGCTTTGGACGACGTGAGATCGGAAGAGCACACGTC474790.17954821797476614No Hit
CTAGAAGACTTAAGAGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG471800.17841750929988978Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTAGCATGGAATAATAGAATAGGACGTAGATCGGAAGAGCACACGTCTG453850.17162947561626743Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATTGCTCTAACTGATCTTGACTCTCCATCAAGATCGGAAGAGCACACGT443080.16755665540609402No Hit
TGAAAGACGTGCTGCTTTGACTGACTTTGAAAGATCGGAAGAGCACACGT437530.1654578483339991No Hit
TGTTACGATCTGCTGAGATTAAGCCTTTGTAGATCGGAAGAGCACACGTC436150.16493598279174845No Hit
ACGTGCTGCTTTGACTGACTTTGAAAGATTCAGATCGGAAGAGCACACGT425470.16089719729085228No Hit
TTGTGTGACTACTCCGACTACATGGCTGTAGATCGGAAGAGCACACGTCT425160.16077996662556407Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACGTGCTGCTTTGACTGACTTTGAAAGATAGATCGGAAGAGCACACG419900.15879082695002905No Hit
GACGTGCTGCTTTGACTGACTTTGAAAGATTCAGATCGGAAGAGCACACG415250.15703236697070627No Hit
GAACTAGAAGACTTAAGAGAAAGAGATCGGAAGAGCACACGTCTGAACTC406310.1536515858491696Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGACTGTGCTTCCTCTGAATTCTTCAAGGAGATCGGAAGAGCACACGT405700.15342090615295734No Hit
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA405370.1532961122189409Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGGTGTCGTCAAGGGTAAGACTTTGTTGAGATCGGAAGAGCACACGT398010.15051282932693752No Hit
CAGATCTGGTGAAACTGAAGACACTTTCATTAGATCGGAAGAGCACACGT394740.14927623488986538No Hit
CGGTAAGATCTTGGGTAAGGGTAGAATTCCAAGATCGGAAGAGCACACGT364180.1377195602730688No Hit
ACGTGCTGCTTTGACTGACTTTGAAAGATTAGATCGGAAGAGCACACGTC335240.12677551042326207No Hit
GCGTGCTTGTGGACTGCTTGGTGGGGCAGATCGGAAGAGCACACGTCTGA308390.11662182215555958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTGCGATGACGACTATACTGGACAAGGTAGATCGGAAGAGCACACGT306170.1157822993267216No Hit
GGGGCTGATCCGGGTTGAAGACATTGTCAAGATCGGAAGAGCACACGTCT305580.11555918289923764Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGACGTGCTGCTTTGACTGACTTTGAAAAGATCGGAAGAGCACACGTC300940.11380450455427898No Hit
GATGTCTGACACCGAAGCTCCAGTTGAAGATCGGAAGAGCACACGTCTGA298700.11295741845671274Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGACGTTATGACCGTAAACAATCTGGTTTAGATCGGAAGAGCACACGTC294290.1112897177021292No Hit
CGTGCTGTCTTCCCATCTATCGTCGGTAGAGATCGGAAGAGCACACGTCT291810.11035187237982372Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGACTCCACTCACGGTAGATACGCTGGAGATCGGAAGAGCACACGTCTG284330.10752320987545073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTCT276930.10472480044599083Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGACGTTGTGCTCGGTGTCCTGGCCACTAGATCGGAAGAGCACACGTC276670.10462647795252333No Hit
AAGGGTGCTCGTTCTAGATTCCAAAAATCTAGATCGGAAGAGCACACGTC275590.10421806144119675No Hit
ATTAACGGTTTCGGTAGAATCGGTAGATCGGAAGAGCACACGTCTGAACT272960.10322349160342924Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAGACGTGCTGCTTTGACTGACTTTGAAAGAGATCGGAAGAGCACACGT271870.10281129345773851No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)