FastQCFastQC Report
Sun 2 Jul 2023
SRR3945924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3945924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28176334
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length45
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAGCACCTGTAGGCACCATC1368700.48576227127347366No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC1327300.4710690893996359Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGAGGTGCACAATCGACCGATCCTGAGGATCTGTAGGCACCATC1285480.4562268462604113No Hit
AAGAGGTGCACAATCGACCGATCCTGACGATCTGTAGGCACCATC1242350.44091967393628995No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAACACTGTAGGCACCA1165150.4135207937271045No Hit
AAGAGGTGCACAATCGACCGATCCTGAGTCACTGTAGGCACCATC1139220.4043180351283457No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAATACTGTAGGCACCA934050.33150160698691317No Hit
AAGAGGTGCACAATCGACCGATCCTGATCGACTGTAGGCACCATC904710.3210886128763238No Hit
AAGAGGTGCACAATCGACCGATCCTGATGGTCTGTAGGCACCATC601190.21336700509015827No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGAACACTGTAGGCACCAT538710.19119236732500403No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGGAACCTGTAGGCACCAT538210.19101491343763882No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAAACCTGTAGGCACCA520980.18489985247903434No Hit
AAGAGGTGCACAATCGACCGATCCTGAACCTCTGTAGGCACCATC519830.18449170853809443No Hit
AAGAGGTGCACAATCGACCGATCCTGAGTCCCTGTAGGCACCATC509590.1808574529248553No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGGAACCTGTAGGCACCA480320.17046930235849703No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATTCCACTGTAGGC466290.16548994627902977No Hit
AAGAGGTGCACAATCGACCGATCCTGAGTCTCTGTAGGCACCATC455420.1616320987677105No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGATACCTGTAGGCACCAT439030.15581516033987955No Hit
AAGAGGTGCACAATCGACCGATCCTGAATCCCTGTAGGCACCATC430230.1526919719222522No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGAATACTGTAGGCACCAT420400.14920322849665255No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGATACCTGTAGGCACCA410230.14559381642764457No Hit
AAGAGGTGCACAATCGACCGATCCTGATGGACTGTAGGCACCATC398100.14128878512016502No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGAACCCTGTAGGCACCAT383400.1360716408316284No Hit
AAGAGGTGCACAATCGACCGATCCTGACCCTCTGTAGGCACCATC376050.1334630686873601No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAACTCTGTAGGCACCA364530.1293745311224661No Hit
AAGAGGTGCACAATCGACCGATCCTGAGGGTCTGTAGGCACCATC340330.12078576297399088No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAACCCTGTAGGCACCA336870.11955778207342375No Hit
TTAAGCCATGCATGTCTAAGTATAAGCAAGACACTGTAGGCACCA334690.11878408312451152No Hit
CAATATAGATGGATACGAATAAGGCGTCCCATCTGTAGGCACCAT333980.11853209860445293No Hit
AAGAGGTGCACAATCGACCGATCCTGAGTATCTGTAGGCACCATC333470.11835109563934045No Hit
AAGAGGTGCACAATCGACCGATCCTGAAGATCTGTAGGCACCATC332160.11788616645444364No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGGAAACTGTAGGCACCA317160.11256254983348792No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGGATACTGTAGGCACCA312830.11102579916890537No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGACTGTAGGCACCATC309450.10982621089031666No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGAAACCTGTAGGCACCAT307890.10927255476173728No Hit
AAGAGGTGCACAATCGACCGATCCTGATGTACTGTAGGCACCATC303550.10773225501940742No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGGACACTGTAGGCACCA288760.10248316903114507No Hit
AAGAGGTGCACAATCGACCGATCCTGAATCTCTGTAGGCACCATC284370.10092512390007868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)