FastQCFastQC Report
Sun 2 Jul 2023
SRR3945925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3945925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000937
Total Bases240 Mbp
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT2464016.158582352084024No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG685221.7126488120157854No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC472821.181773169635013No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC373790.9342561504967462No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG262680.6565462040517009No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA241000.6023588974282774No Hit
TTAACGCGAACTCGGCCTACAATAGTCTGTAGGCACCATCAATAGATCGG196070.4900602033973541No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC189950.47476378658299295No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA173900.4346481836629769No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT155580.38885890980037924No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGAT151740.37926115807372124No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATAGA142280.3556166967887772No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG123870.30960247562008597No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCCTGTAGGCACCATCAATAGA123750.30930254587862793No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA116230.29050694874725597Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG110500.27618530359263344No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATAGA103280.2581395308149066No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA72170.18038274534190366No Hit
TTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGAT71450.1785831668931553No Hit
CATTAACGCGAACTCGGCCTACAATAGTCTGTAGGCACCATCAATAGATC67410.16848553226406715No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGA66270.16563619972021554No Hit
CAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGAT65000.1624619432897844No Hit
AACGCGAACTCGGCCTACAATAGTCTGTAGGCACCATCAATAGATCGGAA61000.15246428524118225No Hit
TAATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGA60600.15146451943632205No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATAGAT58630.1465406728473855No Hit
ATTAACGCGAACTCGGCCTACAATAGTCTGTAGGCACCATCAATAGATCG58330.14579084849374033No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC57110.1427415627889167No Hit
ATGATTAATAGGGACGGTCGGGGGCATCACTGTAGGCACCATCAATAGAT56900.1422166857413651No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTCTGTAGGCACCATCAATAGA56890.14219169159624356No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG56800.14196674429015002No Hit
ACGCGAACTCGGCCTACAATAGTCTGTAGGCACCATCAATAGATCGGAAG54600.13646803236341887No Hit
TAATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGAT54120.1352683133975866No Hit
TAACGCGAACTCGGCCTACAATAGTCTGTAGGCACCATCAATAGATCGGA53950.13484341293052104No Hit
AATGATTAATAGGGACGGTCGGGGGCATCACTGTAGGCACCATCAATAGA53550.1338436471256608No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGAT52920.13226901598300597No Hit
ACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT51840.12956964830988343No Hit
GCATTAACGCGAACTCGGCCTACAATAGTCTGTAGGCACCATCAATAGAT46100.11522300901013938No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAGAT45490.11369836615772755No Hit
AATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGAT44930.11229869403092324No Hit
TTGCTCCACACAATATAGATGGATACGAATCTGTAGGCACCATCAATAGA44440.1110739809199695No Hit
TTACCCAATCCTAATTCAGGGAGGTAGTGCTGTAGGCACCATCAATAGAT41640.10407562028594802No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)