FastQCFastQC Report
Sun 2 Jul 2023
SRR3945926.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3945926.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28658776
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length45
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATA22056707.6963161301794605No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAA4872111.700041202038775No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAAT2307660.8052193157167634No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCAT1722190.6009293627892552No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATTAATA1295560.45206396811922467No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACTATCAATA1112440.3881673104252603No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCA1091850.38098277470049663No Hit
CAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAAT988710.3449937987581884No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATC979220.3416824221662502No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCATCATCAATA916910.31994039103414607No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACATCAATAG824040.2875349596228394No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGTACCATCAATA802860.28014455327750215No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAAT742400.25904804866753556No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCA712370.24856958301359416No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCAT674530.23536594863646654No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCA648900.22642278930544696No Hit
TTACCCAATCCTAATTCAGGGAGGTAGTCTGTAGGCACCATCAAT558250.19479198972070544No Hit
AATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAAT549370.19169346241444504No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAA540850.18872055108006008No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAG498750.17403046103574No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCACAATAG495720.1729731932724552No Hit
ATGTACACGGAGTCGACCCGCAACGCGCTGTAGGCACCATCAATA464640.1621283477005438No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACGATCAATA462980.16154911849689602No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAAT454800.15869484446928228No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATGAATA386020.13469521517597263No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACATCAAT383520.13382288203794887No Hit
TAATGATTAATAGGGACGGTCGGGGGCATCACTGTAGGCACCATC377190.1316141345324727No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCCCATCAATAG370030.12911577242517266No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCAT361420.12611145709781885No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAAT341650.11921304664232693No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATC322880.11266356944204456No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAAT309210.10789365184333065No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCAGCATCAATA306950.10710506268655716No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGACCATCAATAG297880.10394023806180697No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCAGCAATA297540.10382160075503573No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCA287900.1004578841748161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)