FastQCFastQC Report
Sun 2 Jul 2023
SRR3945927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3945927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6006798
Total Bases360.4 Mbp
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT3842816.397435039433655No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2410444.012853437055816No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC1103071.8363693934771903No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG383540.6385099016148038No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA309290.5148999516880707No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG226110.3764235121607219No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA220440.36698420689358957No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCCTGTAGGCACCATCAATAGA201410.33530343454199724No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC194520.32383309710098457No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG185590.30896660750036875No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT166570.27730248295348037No Hit
ACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT161050.2681128947569071No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATAGA155060.2581408597392488No Hit
AATGACCCACTCGGCACCTTACGAGAAATCCTGTAGGCACCATCAATAGA153090.25486124221257317No Hit
CAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGAT140020.23310256146452735No Hit
TTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGAT139680.23253653610459352No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGA133340.2219818279222974No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGAT123720.20596663979710989No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC116720.1943131765043539No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATAGA115910.19296470432333498No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGAT115700.1926151004245523No Hit
ATGATTAATAGGGACGGTCGGGGGCATCACTGTAGGCACCATCAATAGAT113300.1886196272956074No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTCCTGTAGGCACCATCAATAGA112110.1866385385358389No Hit
AATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGAT108900.1812945932258751No Hit
CAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC107060.17823139716035066No Hit
TAATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGAT97160.16175007050345291No Hit
TTGCTCCACACAATATAGATGGATACGAATCTGTAGGCACCATCAATAGA96980.16145041001878205No Hit
ATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGATC94130.15670578567816No Hit
TAATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGA90720.15102888427411743No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC81040.13491380932070632No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA80270.13363192835850315No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA80220.13354868933498348No Hit
AATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATC79870.13296601617034567No Hit
ATATAAAGAATGGTACAGCAATTAATATACTGTAGGCACCATCAATAGAT77990.1298362288860055No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT76280.12698945428163225No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAGAT75740.12609047282761965No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATAGAT71180.11849907388262432No Hit
AATGATTAATAGGGACGGTCGGGGGCATCACTGTAGGCACCATCAATAGA67820.11290541150210147No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTCTGTAGGCACCATCAATAGA66180.11017517153065577No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAG63230.10526406914299431No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA62100.10338286721144943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)