FastQCFastQC Report
Tue 4 Jul 2023
SRR3945928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3945928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26362552
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length45
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATA476234118.064795092675396No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCC9158583.474087030724491Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAA5560522.1092495142351924No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAAT3948361.4977153956870337No Hit
CAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAAT3492211.3246858650103375No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAG1405010.5329567486486134No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCA1343320.5095561309845875No Hit
AATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAAT1136660.4311646307990213No Hit
ATGTACACGGAGTCGACCCGCAACGCGCTGTAGGCACCATCAATA1049920.39826189816524593No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATC1032050.39148334349421104No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCAT1010450.3832899030412533No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAAT939010.3561908573949897No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCA937200.3555042774311076No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAAT821840.3117452361971633No Hit
ACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAA685900.2601796669760955No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAAT679480.25774439439702196No Hit
TTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAA568370.21559748843738646No Hit
AATGACCCACTCGGCACCTTACGAGAAATCCTGTAGGCACCATCA551390.2091565338590892No Hit
AATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATA542710.20586398464003028No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCA476670.18081329910700603No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCA471170.17872700639907702No Hit
TTGCTCCACACAATATAGATGGATACGAATCTGTAGGCACCATCA427030.16198355910307924No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCAT406550.1542149637106453No Hit
TAATGATTAATAGGGACGGTCGGGGGCATCACTGTAGGCACCATC398730.15124863480591713No Hit
CAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAA393280.1491813083953329No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATC380600.14437145538868923No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCAT372970.14147719841387132No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAG352450.1336934299835615No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCCTGTAGGCACCATCA343680.13036674142928195No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAA342640.12997224244450992No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAA334970.12706281243181616No Hit
TTACCCAATCCTAATTCAGGGAGGTAGTCTGTAGGCACCATCAAT334470.12687314945836806No Hit
AATGATTAATAGGGACGGTCGGGGGCATCACTGTAGGCACCATCA324600.1231292023625027No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCA307870.11678307927092946No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAA295800.11220461509189247No Hit
ATATAAAGAATGGTACAGCAATTAATATACTGTAGGCACCATCAA281020.1065981775967668No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAA278770.10574469421625039No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATC272150.10323355644779762No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)