FastQCFastQC Report
Mon 26 Jun 2023
SRR403883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR403883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20762604
Total Bases830.5 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCA10520635.067105262904403No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAG4208162.0267977947274822No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA3742901.8027122224168028No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAG2387131.149725728044517No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGG2291351.1035947128789818No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTA2080971.0022683089269535No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGG1977530.952447968472548No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTA1534600.7391173091775964No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGC1332200.6416343537641039No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAG1269360.6113684006110216No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGG1086080.5230943093650489No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCAC1071270.5159612927164627No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTA1070720.5156963933811No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA964640.4646045361169533Illumina Single End Adapter 1 (95% over 22bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCAC883340.4254475979987867No Hit
TGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCAC880090.4238822837443704No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGC837860.4035428311400632No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCA817600.3937849028956098No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA814940.39250375338276455No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCAT788740.3798849123163935No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT778530.3749674173817504No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGCTGTAGGCACCA707610.3408098521746116No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAG691980.3332818946987574No Hit
TTGCAGTTAAAAAGCTCGTAGTTGGATCTTGCTGTAGGCA670850.32310494386927574No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGACTGTAGGC659990.3178743860837494No Hit
GGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCA617170.2972507687378712No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCA602750.2903055897998151No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA551760.265747013235912No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGC544110.26206250429859373No Hit
ATGTACACGGAGTCGAGCACCCGCACGCGACTGTAGGCAC526010.2533449079893832No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC524340.25254057728019086No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT521940.25138465290769885No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACC496130.23895364955185774No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGG493980.23791813396816702No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTG487550.23482121992019883No Hit
TATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGC470580.22664787133636993No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTA455880.2195678345548564No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCAT437850.21088395270651025No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGC433430.2087551253205041No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGG429100.20666964509846644No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTA422210.20335117887910398No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGG414140.19946438317659962No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA400610.19294785952667592No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTA380160.18309842060273362No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGG379750.1829009501890996No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA376630.18139824850485997No Hit
TGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCAC362880.1747757651207912No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAG358930.17287330625773145No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACC351150.16912618475023652No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGC342080.16475775389252717No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGT341170.1643194659012906No Hit
GCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA329880.15888180499902613No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGG325400.15672407950370773No Hit
TGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAG322350.15525509228033246No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAG318810.15355010383090675No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCAT310840.14971147164392287No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCAT304440.14662900665061088No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCA302890.1458824721600431No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA298660.14384515545352597No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCACTGTAGGCACC295650.14239543363635893No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACTGTAGGCAC295290.1422220449804851No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCA291710.1404977911248512No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT290380.13985721636842854No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACC289410.1393900302678797No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACC288960.13917329444803744No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGG288480.13894210957353903No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCA284790.1371648758508326No Hit
TACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCA276360.1331046914924544No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACTGTAGGC263440.1268819652872058No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGACTGTAGGCA261630.12601020565628474No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATC254200.12243165645311156No Hit
AGTTCAACTTTAAATTTGCCCACAGAACCCCTGTAGGCAC253640.12216194076619677No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACTGTAGGCA252640.12168030561099175No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCA247870.11938290592066389No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA244990.11799579667367349No Hit
GTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAG242020.11656534026271465No Hit
AAAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTA240480.11582362212369894No Hit
AATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGC238570.11490369897725738No Hit
TGAAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGC231650.11157078370323877No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAG229860.11070865677542181No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGC228280.10994767323019791No Hit
AAACCTGCATTAAAAATTTCGGTTGGGCTGTAGGCACCAT225680.10869542182666492No Hit
ATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAAT224310.10803558166403406No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCA217880.10493866761606588No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGT216290.10417286771928992No Hit
GTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCAC215840.10395613189944768No Hit
AAAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCA209370.10083995244527132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)