FastQCFastQC Report
Mon 26 Jun 2023
SRR403885.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR403885.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19742795
Total Bases789.7 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA6778053.4331765081894434Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA6136183.108060434198906No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA3653411.8505029303095129No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC2811721.4241752497556703No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA2765391.4007084609853873No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCA2375781.2033655822288587No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCAC1877050.9507519072147586No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACC1729260.8758942186250731No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCAC1286130.6514427161908939No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACC1201980.6088195718995209No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCAT1094800.5545314125988747No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCT1037280.5253967333399349No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCAC970840.49174395013472005No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT949040.4807019472166935No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACC929290.47069829778407773No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCA822690.4167039165427185No Hit
GCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA750430.38010322246672773No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA748870.3793130607900249Illumina Single End Adapter 1 (95% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT733380.3714671605514822No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC726500.36798234495166465No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCA703490.3563274602202981No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGC701920.355532233404642No Hit
CTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCA669190.33895403361074256No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGC657230.3328961274226876No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCA652840.33067253142222264No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAG643130.3257542814986429No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA613230.31060951602850556Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG538200.27260577846247197Illumina Single End Adapter 1 (95% over 22bp)
GCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC511820.25924394190386923No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGC504880.2557287354703323No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCAC494620.25053190290432537No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA470340.23823374552589946No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGCTGTAGGCACCA463840.23494140520630438No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCA460490.2332445836569746No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA435200.22043484724427317No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC424880.21520762384454684No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCAT415380.2103957418389848No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACC411470.20841527250827452No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACC409320.20732626763333156No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCAT395410.20028065934939812No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCA389880.1974796375082657No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC384340.19467355052818003No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCAT378730.19183200757542182No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCA366600.1856879940251621No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATC364070.18440651386999662No Hit
TTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCA361120.1829122978787958No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGT361060.18288190704507643No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA358440.18155484063933197No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGG344830.1746611865239952No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGG343540.1740077835990294No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCACTGTAGGCACC329730.1670128267046282No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGG314090.15909094938178714No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCAT305720.1548514280779393No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA305680.15483116752212642No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCA304400.15418282973611386No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTA301360.152643027494334No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCAC299830.15186806123449087No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCAT293790.14880871730674405No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCTGTAGGCACCAT291880.14784127576667844No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCA291170.14748165090099957No Hit
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG287830.14578989449062302Illumina Single End Adapter 1 (95% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCA287580.14566326601679244No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATC287070.14540494393017808No Hit
GTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA285110.1444121766953463Illumina Single End Adapter 1 (95% over 22bp)
TGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCAT284270.14398670502327557No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCTGTAGGCACC283780.14373851321456763No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAG283090.14338901862679523No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGC281420.14254314042160696No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACC270100.13680940312655832No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCA260590.13199245598204307No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCAC253690.1284975101043191No Hit
TAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA240590.12186217807559668Illumina Single End Adapter 1 (95% over 22bp)
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCAC233760.11840268817054525No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCA229910.11645260967355434No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC228980.11598155175090456No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACC226730.11484189548642935No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGC225470.11420368797832323No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCA221270.1120763296179695No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGACTGTAGGC220470.11167111850171164No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCAC217380.11010599056516567No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCA209530.10612985648688546No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACC205960.1043216018805848No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAG204550.10360741728818032No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCA203800.1032275318666886No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCAT201260.1019409865725699No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGC200260.10143447267724756No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)