FastQCFastQC Report
Mon 26 Jun 2023
SRR403887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR403887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19706845
Total Bases788.2 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCA7431993.7712733824211844No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA6683033.391222694449568Illumina Single End Adapter 1 (95% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA3562341.807666321016885No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA3103271.5747168052521854No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC2820751.4313554503524029No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCAC1953430.991244412791596No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACC1783550.9050408627053188No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC1021680.518439151472496No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACC881900.44750948211141867No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT871950.44246047502783925No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACC832180.4222796698304574No Hit
CTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCA791070.40141889784996027No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA790840.40130218713345545Illumina Single End Adapter 1 (95% over 22bp)
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCAC765350.3883675951173311No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCAC755430.38333381117068716No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC706330.35841861038639117No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCT693010.3516595375870668No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA689160.34970590168035526No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT681420.3457783323510181No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCAC667980.3389583670039522No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGG638580.32403969280724537No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA631750.32057389196494923Illumina Single End Adapter 1 (95% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCA629020.3191885865038265No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACC602950.30595968050695077No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCA567170.28780355252197903No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCA545080.27659424935853505No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCAT540900.2744731589455339No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCAT512540.2600822201625882No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCA477460.24228129870610945No Hit
GCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC456170.23147794586094325No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCA455800.23129019383873978No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA450490.22859569860117132No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG440460.22350609648576422Illumina Single End Adapter 1 (95% over 22bp)
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATC413640.20989661206550314No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCA403950.20497953883536404No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC402090.20403570434536833No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGC392550.1991947467999063No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATC355050.1801658256306375No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCA353750.17950615636343614No Hit
AGAACTCTGAACCTGTAGGCACCATCAATTCGTATGCCGT351720.1784760574308064No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACC341280.17317840577728197No Hit
TGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCA340570.17281812486981046No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCAC332580.16876369606601158No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCA331230.1680786549039179No Hit
ATGTACACGGAGTCGAGCACCCGCACGCGACTGTAGGCAC322290.1635421600971642No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGC321660.1632224742215205No Hit
CTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACCAT320740.1627556313555011No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA311190.15790959943106062No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA307520.15604730234596154No Hit
TATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGC306570.1555652363430067No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCA304670.1546011043370971No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACC304120.15432201349328115No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC303040.1537739805636062No Hit
TGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT299710.15208421236377512No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGC299090.1517696008671099No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCAT297960.15119619604254259No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAG295910.15015594835195586No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGG289330.1468170069841215No Hit
GAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATC289030.14666477561476735No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCAC282600.14340194993161004No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACC280280.14222469400860463No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCAC275200.139646909487541No Hit
GTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCA270630.13732791829437946No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATC269010.13650586889986704No Hit
GTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAA261710.13280157224558267Illumina Single End Adapter 1 (95% over 22bp)
GACTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG259730.1317968452078453Illumina Single End Adapter 1 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC258840.13134522547876132No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGG257370.13059929176892598No Hit
TCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACC253980.12887907729522408No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA244540.12408886353954679No Hit
GCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCA244130.12388081400142945No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACC238220.12088185602515268No Hit
TAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA236260.11988727774537222Illumina Single End Adapter 1 (95% over 22bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCAC229330.11637073311329135No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGC227580.1154827167920588No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCAC216960.11009372631692185No Hit
TGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCAT214730.10896213980472268No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCA208920.10601392561823061No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCAT208810.10595810744946743No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCAT201670.10233500085883865No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACC197180.10005660469750485No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)