FastQCFastQC Report
Tue 27 Jun 2023
SRR403891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR403891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25445068
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC7435002.921980794077658No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA6210732.440838436745384Illumina Single End Adapter 1 (95% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG4461941.7535578997077153No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACC3903571.5341165525672793No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA3336421.3112246349665877No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC2323260.9130492400334713No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCA2221650.8731161575201922No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC2074570.8153132072588684No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGG2055330.8077518205099707No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC1953040.7675514956375828No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1796420.7059992922793525No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1542700.6062864520542842No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA1281220.503523904907623No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC1244340.4890299369606715No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCAC1211400.4760844026826731No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG1026620.4034652216295904No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG983200.3864010109935646No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCA930250.365591477295325No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCAC912470.35860387561157236No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC861830.3387021799273635No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCAC832200.3270574871326734No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA793230.3117421419349321No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCA704470.27685915400186784No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCAT701680.27576267432258383No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGC693780.2726579469152922No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCA690930.27153788702785153No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC665020.26135516713887347No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCA661080.25980673346992034No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAG661020.2597831532617637No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA659520.2591936480578476Illumina Single End Adapter 1 (95% over 22bp)
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA640820.25184448318235975Illumina Single End Adapter 1 (95% over 22bp)
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCA624060.2452577450372701No Hit
CTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCA599970.23579029146237693No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA591350.23240260155720552No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGACTGTAGGC587970.23107424983104782No Hit
ACTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTAT583440.22929394411522105Illumina DpnII expression PCR Primer 2 (95% over 24bp)
TGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGC564010.22165788670716072No Hit
ACCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTG555790.21842739818970025Illumina Single End Adapter 1 (95% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGC540360.2123633546587496No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACC505980.19885189538499168No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG505630.1987143441707446No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCA485570.19083069457703944No Hit
TTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACC458400.18015279031677178No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCA447410.1758336821894129No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGC423830.1665666603838512No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGC419680.16493569598634988No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAACCTGT390740.1535621755854612No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACC382000.1501273252639765No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA375750.14767105358099258No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACC374300.14710119855054032No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTACTGTAGGCAC371340.14593790828147915No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCTGTAGGCACC363840.14299038226189845No Hit
ATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCTT347300.13649010488004984No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC346520.13618356217401345No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACC344760.13549187606808516No Hit
TGATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTC340170.1336879901441018No Hit
ATGCAGCTCAAAACGCTTAGCCTAGCCACACCTGTAGGCA329570.12952215336976108No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCA328640.12915666014333307No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGG316190.12426376695082912No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACC314520.12360745115713584No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCA312760.12291576505120756No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCT311690.12249525133908072No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGC305220.11995251889285578No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGC282010.11083090837092674No Hit
GTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCAT272330.10702663478832125No Hit
CTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACC264870.10409482890751166No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCA259340.1019215197224075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)