FastQCFastQC Report
Tue 27 Jun 2023
SRR403893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR403893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25291675
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCA2552381.0091779211934362No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA2303280.9106870146006542Illumina Single End Adapter 1 (95% over 22bp)
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC1920390.7592972786499906No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG1912530.7561895366756057No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACC1908600.7546356656884132No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC1562140.6176498788633018No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1494330.5908386850613888No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCAC1488630.5885849790494303No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGG1317760.5210251990032293No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCA1028160.40652111811495284No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA999780.39530003449751744No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAG944280.37335605490739543No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG854530.3378700698945404No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTA833600.3295946195734367No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGG757710.29958869865281756No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA665830.26326053928812543No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCAC628850.24863912730177024No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG607740.24029250731713103No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAG586070.2317244706014924No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGACTGTAGGC578550.22875116021378578No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAG561480.22200190378849957No Hit
ATGCAGCTCAAAACGCTTAGCCTAGCCACACCTGTAGGCA556120.21988262936321934No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC538160.2127814784904519No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGC538120.21276566300966623No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAACCTGT537510.21252447692768472No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTA527860.20870899218814096No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCAC514500.203426621605726No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGG507980.20084869823766122No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGG501580.19831822131195345No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCA487380.19270372563303934No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCCTGTAGGCAC480660.19004672486104618No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGC479400.18954853721629747No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCAC470770.1861363472367884No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCA469070.18546418930339728No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTA443690.1754292667448874No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC438070.17320719169450027No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGG435330.17212383126068162No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTACTGTAGGCA425340.16817391493445966No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCA418510.1654734215903059No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAG395660.15643882819148988No Hit
AAAACATCACCTCTAGCATCACCAGTATTAGAGGCCTGTA373390.14763355926406616No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAACTGTAGGC356630.14100687281486893No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC341360.13496931302493803No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCA336400.13300819340751452No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGACTGTAGGCA334730.13234789708471265No Hit
ACTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTAT328050.12970671179350518Illumina DpnII expression PCR Primer 2 (95% over 24bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTACTGTAGGCAC322350.1274530057815467No Hit
AAATGCAGCTCAAAACGCTTAGCCTAGCCACACCTGTAGG314430.12432154058598334No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGC312990.12375218327769909No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACC312710.12364147491219937No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACC310860.12291000892586197No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG306330.12111890572688445No Hit
TCCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCA294930.11661149370296747No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTACTGTAGGC285460.11286717862695927No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCCTGTAGGCACC278740.1102101778549661No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAG278530.11012714658084133No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAACTGTAGGCA277460.10970408246982455No Hit
TCAGGTCGGTTTCTATCTACTTCAAATTCCTCCTGTAGGC275050.10875119975248773No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCCTGTAGGCACC274660.10859699881482741No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAGACTGTAG272060.10756899256375863No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC270920.10711825136136693No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCA267320.10569485809065632No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCA263570.10421215676699941No Hit
AGTTCAACTTTAAATTTGCCCACAGAACCCCTGTAGGCAC262970.1039749245552143No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGA259150.1024645461401825Illumina Single End Adapter 1 (95% over 22bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTA256330.1013495547447925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)