FastQCFastQC Report
Mon 3 Jul 2023
SRR4045263.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045263.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110322605
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA90635848.215527543063365Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT26207582.3755403527681387No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA18750561.699611788535994No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATA14361011.3017286892382571No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGATC9216970.8354561605937424No Hit
CTGGAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7687160.6967892029017988Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATAG7364530.6675449695916806No Hit
TCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT7167200.6496583361134375No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGAT6565700.5951364183251474No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGA6471730.5866186716675155No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA5904770.5352275719015156Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5513450.4997570534162061Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC5432470.4924167626389895No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG5325100.4826843963664564No Hit
GCTTGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT5218260.47300007101899016No Hit
TCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA4913840.4454064513795699No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG4899260.44408487272395347No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAG4835420.4382982073347525No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC4484570.4064960213729544No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3869670.3507594839697631Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGA3806160.34500273085466027No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGAACTGTAGGCACCATCAAT3707460.3360562416016192No Hit
GCTTGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA3647580.3306285235015979No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAATAGATC3577770.32430071788098186No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3534170.3203486719698107Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT3492020.3165280587781624No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGAT3373270.30576417226551167No Hit
CCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT3119330.28274622413058503No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG3060790.27743996799205384No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTAGGCACCATCA2862290.2594472819056439No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAG2834770.25695277953235424No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAG2775530.2515830731154327No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2772080.25127035388622304Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATAGA2660670.24117178886412263No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGAT2585940.23439801842967722No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG2459950.2229778747519604No Hit
CCGAGCCTGGTGATAGCTGGTTGTCCAAGACTGTAGGCACCATCAATAGA2405150.21801062438654345No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAATAGAT2400090.21755196951703595No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCCCTGTAGGCACCATCAAT2301420.20860819956164015No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2234540.20254597867771523No Hit
CCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA2177650.1973892839096756No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACTGTAGGCACCATCAATAGA2116300.19182832022503457No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT2108690.1911385250556765No Hit
TCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATAG2001060.18138259153688402No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC1973970.178927065763177No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATA1911090.17322741789862559No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1888750.17120244758542458Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATA1819450.1649208700247787No Hit
TAGAATCTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAA1785420.16183628006245865No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCTGTAGGCACCATCAATAG1778970.16125163106871887No Hit
TTTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAA1714960.15544955632619445No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1705220.15456669102401996No Hit
CAGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAAT1654770.1499937388171717No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAATAGATC1650380.1495958149284093No Hit
AGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAA1610390.1459709911672227No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCCCGCTGTAGGCACCATCAATA1590390.1441581260703552No Hit
CTGTTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGATC1576080.1428610210935465No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATA1533880.1390358757391561No Hit
CATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC1527900.13849382907519273No Hit
TATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGAT1519550.13773695789725052No Hit
CGAGCCTGGTGATAGCTGGTTGTCCAAGACTGTAGGCACCATCAATAGAT1453110.1317146200454567No Hit
AGTTCAACTTTAAATTTGCCCACAGAACCCCTGTAGGCACCATCAATAGA1449710.1314064329789892No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTGTAGGCACCATCAA1442700.13077102376253716No Hit
AGAATCTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAAT1400870.1269794164124388No Hit
TCCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1389330.12593339325154623No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCCTGTAGGCACCATCAATA1377150.12482935840755392No Hit
TAGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAAT1374530.12459187307986427No Hit
TCTTGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT1341950.12163871583706712No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGA1328230.12039509038061602No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1326640.12025096760541504No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCACTGTAGGCACCATCAATAGA1320490.1196935115881283No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1306510.11842631888541791Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA1266430.11479333723129544No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAATAGAT1262310.11441988702134073No Hit
TTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGATC1260020.1142123139677494No Hit
TGGCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAG1232490.1117169051619113No Hit
AGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAATAGATCGG1230300.11151839643380429No Hit
AGAATCTTAGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAA1204800.10920699343529823No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTAGGCACCATCA1193780.10820810476692425No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCCCGCTGTAGGCACCATCAAT1193700.10820085330653677No Hit
AGTTCAACTTTAAATTTGCCCACAGAACTGTAGGCACCATCAATAGATCG1193110.10814737378617917No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGCTGTAGGCACCATCAATA1191920.10803950831291555No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT1186510.1075491283042129No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1182930.10722462545187361Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGAT1168990.10596105847935698No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATAG1155650.10475187745974636No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAATAGAT1154220.10462225760532033No Hit
TTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAATAGATCG1128840.10232173179739545No Hit
TCATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGAT1111750.1007726385721222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)