FastQCFastQC Report
Mon 3 Jul 2023
SRR4045264.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045264.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48736545
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA39475478.099767843617146Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT10994232.2558492810682416No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA7740461.5882250167712955No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATA7021521.440709430674661No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGATC4090580.8393249870297536No Hit
TCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT3082140.6324083908697262No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC3056950.6272397848472845No Hit
CTGGAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3019630.6195822867624285Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGAT2933830.6019774278213608No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATAG2822390.579111629681587No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGA2571350.5276020284162531No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA2453630.5034476695055015Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2405840.49364188618622845Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA2127130.43645482050481826No Hit
GCTTGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT2117650.4345096682581828No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG2098020.43048188992469616No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG2040760.4187330062071491No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAG1866450.38296723741906613No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC1858060.3812457366438265No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGAACTGTAGGCACCATCAAT1817640.37295216556692723No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG1781040.36544240056409416No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1690740.346914209860383Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGAT1686330.34600934473299244No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAATAGATC1673720.34342196394922947No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1530730.3140825842291447Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGAT1493930.30653178225908295No Hit
GCTTGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA1471080.3018433087532159No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAG1469490.3015170648637486No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGA1416080.29055814276535197No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTAGGCACCATCA1358040.27864921487561334No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT1348100.2766096776043522No Hit
CCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT1336230.27417413359933496No Hit
CCGAGCCTGGTGATAGCTGGTTGTCCAAGACTGTAGGCACCATCAATAGA1187840.24372675576407807No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1186450.2434415488418393Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT1181840.24249564674722018No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAATAGAT1127200.23128434730036773No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1124150.23065853355013163No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATAGA1094200.22451324770764938No Hit
CATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC1037820.2129449266459081No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAG1036370.2126474086335008No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACTGTAGGCACCATCAATAGA958280.19662452477909545No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCCCTGTAGGCACCATCAAT956950.19635162894702526No Hit
CCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA940300.19293530142524465No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC909090.18653148268922223No Hit
TGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATA900860.1848428114877655No Hit
AGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAA869010.17830767445661155No Hit
TCCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT838940.17213776643379214No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT831940.17070147258079127No Hit
TATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGAT825370.16935340820733188No Hit
CAGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAAT819560.16816128430934119No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA818470.16793763283794533Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTAGTTCAACTTTAAATTTGCCCACAGAACCTGTAGGCACCATCAATAG797170.16356719582809984No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAATAGATC787960.161677443487223No Hit
TCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATAG780050.160054431433332No Hit
CGAGCCTGGTGATAGCTGGTTGTCCAAGACTGTAGGCACCATCAATAGAT756660.1552551581159477No Hit
AAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGA750020.1538927308039583No Hit
TCATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGAT734710.15075135096260928No Hit
TAGAATCTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAA729840.14975210081059295No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT715800.1468713057111455No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCCCGCTGTAGGCACCATCAATA711120.14591104067799637No Hit
TTTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAA695150.14263423884479295No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATA674990.13849771254815046No Hit
CTGTTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGATC671590.13780008410526434No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC666240.1367023452318994Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTTCAACTTTAAATTTGCCCACAGAACCCCTGTAGGCACCATCAATAGA658460.135106007206707No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGA646810.1327156038656413No Hit
TAGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAAT643240.13198309400061084No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAATAGAT599560.12302062035788544No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATAG598630.12282979846027248No Hit
ACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGAT595170.12211985892721777No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA593320.12174026698035326No Hit
AGAATCTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAAT588930.12083950554968555No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAACTGTAGGCACCATCAATAGATC588780.12080872782426412No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA583200.11966379643858628No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTGTAGGCACCATCAA578250.11864813149967852No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCCTGTAGGCACCATCAATA567030.1163459576381543No Hit
TCTTGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT558420.11457931619896322No Hit
TTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGATC556220.11412790955944865No Hit
AGTTCAACTTTAAATTTGCCCACAGAACTGTAGGCACCATCAATAGATCG555670.11401505789957003No Hit
AGAATCTTAGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAA543220.11146050668958993No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAATAGAT540800.1109639593861239No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGCTGTAGGCACCATCAATA535990.10997702032427617No Hit
AGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAATAGATCGG531010.10895519984028414No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATC526840.10809957907356789No Hit
TTAGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAATAGATC513080.10527623572824049No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCCCGCTGTAGGCACCATCAAT512640.10518595440033758No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT510060.10465657752308868No Hit
TCGAGCCTGGTGATAGCTGGTTGTCCAAGACTGTAGGCACCATCAATAGA509390.10451910368287289No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGA501420.10288378053881332No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC499980.10258831437476744Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATA497790.10213895958361431No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCACTGTAGGCACCATCAATAGA497120.10200148574339853No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTACTGTAGGCACCATCAAT497030.10198301910814564No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC493790.1013182202390424No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)