FastQCFastQC Report
Mon 3 Jul 2023
SRR4045265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61586060
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA51160378.307134763938464Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT15213352.4702586916584695No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA11010101.787758463522427No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATA7339491.1917453397733189No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGATC5126390.83239453863423No Hit
CTGGAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4667530.7578874180293398Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATAG4542140.737527291078533No Hit
TCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT4085060.6633091969189131No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGA3900380.633321891350088No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGAT3631870.589722739204294No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA3451140.5603768125449168Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG3284340.5332927613813906No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3107610.5045963323518342Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTTGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT3100610.5034597114996479No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAG2968970.48208474450224614No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG2801240.4548496851397865No Hit
TCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA2786710.45249038499946254No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC2626510.42647800492514054No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGA2390080.3880878237705091No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATC2375520.38572365239796147No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2178930.353802467636345Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTTGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA2176500.35340789782622883No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT2143920.34811773963133863No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2003440.3253073828720331Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAATAGATC1904050.30916899051506136No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGAACTGTAGGCACCATCAAT1889820.30685840269697395No Hit
CCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGAT1783100.2895298059333557No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAG1739160.28239507446977447No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGACTGTAGGCACCATCAATAGAT1686940.27391588291246427No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1641220.26649212500361286No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1585630.2574657316931786Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATAGA1566470.2543546380463371No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTAGGCACCATCA1504250.24425170241447494No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAG1365280.2216865310104267No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCCCTGTAGGCACCATCAAT1344470.2183075195912841No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1335800.2168997334786476No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG1279750.207798647940784No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAATAGAT1272890.20668475950564136No Hit
CCTGGTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGA1237350.20091397306468378No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATA1236100.2007110050553648No Hit
TCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATAG1221010.19826077524686594No Hit
CCGAGCCTGGTGATAGCTGGTTGTCCAAGACTGTAGGCACCATCAATAGA1217310.19765998993928172No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACTGTAGGCACCATCAATAGA1158020.1880328113212633No Hit
AATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGAT1092010.17731447668514597No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1070280.1737860808111446Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC1064880.17290925901088655No Hit
TAGAATCTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAA1055580.17139917702155327No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATA1036090.16823449982025154No Hit
TTTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAA1019810.16559104446688097No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT989420.16065648622431764No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCTGTAGGCACCATCAATAG981800.15941919323950907No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT926850.1504967195498462No Hit
TGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATA918590.1491555069442663No Hit
GCTTGTCCGATGGTAGTGGGTTATCAGAACTCTGTAGGCACCATCAATAG909610.14769738476531866No Hit
CTGTTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGATC904490.14686602779914806No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCCCCCGCTGTAGGCACCATCAATA879270.14277094524312806No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTGTAGGCACCATCAA864450.1403645565246421No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAATAGATC862420.14003493647750806No Hit
CAGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAAT835210.13561672885065224No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCACTGTAGGCACCATCAATAGA823370.13369421586638275No Hit
AGAATCTTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAAT811940.1318382763891699No Hit
TTTAGTTCAACTTTAAATTTGCCCACAGAACCCTGTAGGCACCATCAATA810120.13154275496760146No Hit
AGTTCAACTTTAAATTTGCCCACAGAACCCCTGTAGGCACCATCAATAGA791250.1284787498989219No Hit
TGGCTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAG784550.12739084136897214No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTAGGCACCATCA754900.12257644018792566No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCCTGTAGGCACCATCAATA752970.12226305758153712No Hit
AGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAA741380.12038113819913142No Hit
TAGTACATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAAT731290.11874278042790852No Hit
TTGGTCCGATGGTAGTGGGTTATCAGAACTGTAGGCACCATCAATAGATC703800.11427910796696526No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA703780.11427586047881615No Hit
AGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAATAGATCGG699290.11354679938934233No Hit
CGAGCCTGGTGATAGCTGGTTGTCCAAGACTGTAGGCACCATCAATAGAT696450.11308565607216958No Hit
TATAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGAT694180.11271706616724629No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA683230.11093906640561191No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC682950.11089360157152447Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATAAGGCCTACTTCACAAAGCGCCTTCCCCCGCTGTAGGCACCATCAAT681060.11058671394143416No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT676450.10983816792306571No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAATAGAT662750.10761363854092955No Hit
AGAATCTTAGTTCAACTTTAAATTTGCCCACAGACTGTAGGCACCATCAA661580.10742366048420698No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGCTGTAGGCACCATCAATA655930.10650624508208514No Hit
TTAGTTCAACTTTAAATTTGCCCACAGCTGTAGGCACCATCAATAGATCG652240.10590708351857546No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC640270.1039634618613368Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTTCAACTTTAAATTTGCCCACAGAACTGTAGGCACCATCAATAGATCG637440.1035039422882386No Hit
CTGTTCCGATGGTAGTGGGTTATCAGAACCTGTAGGCACCATCAATAGAT619590.1006055591151634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)