FastQCFastQC Report
Mon 3 Jul 2023
SRR4045266.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045266.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30473759
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA15612785.123352192947381No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT11451473.7578134026721153No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA7152812.347203047710655No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA6729422.2082671192615257No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG6086251.9972101242908695No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT3729701.2239054591197627No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA3060901.0044379493845836No Hit
TGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCAT2907550.9541159658052032No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA2565520.8418784174279255No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA2359750.7743547489497439No Hit
TCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCAT2209570.7250730046135758No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAA2010290.6596790372989431No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC1876150.615660837903194No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1807500.5931332593396175No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1799920.5906458733889705No Hit
TCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATCA1731220.5681018872663527No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC1729880.5676621646840484No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1673830.5492692909988558No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA1650020.5414560113834332No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA1591830.522360894171277No Hit
TCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTCTGTAGGCACCATC1587410.5209104659520344No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1462840.4800326733567723No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1456850.47806704778363573No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT1417800.46525274417245344No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1381120.4532161588598243No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA1368610.44911098758771445No Hit
TCGACAGAGTGGTTCATTTCCACCTTTCGGGCGCCACTGTAGGCACCATC1317200.43224073538154584No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAATA1303500.42774506420425523No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA1267670.4159874073953265No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1266460.4155903444665294Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATC1191510.39099541346376077No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAAT1147840.37666505139717094No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA1139080.37379044705315156No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCATCAA1128050.37017093952866137No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATCAAT1116200.3662823480358954No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1098420.36044782004084236No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1096450.3598013622146188No Hit
AGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATCAA1083530.35556164895837106No Hit
AGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCATCA1054190.345933693312991No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1020880.33500297747973923No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA1002840.3290831301776719No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAG968120.31768972117945804No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC960830.31529749907125015No Hit
GCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATCA907110.2976692176373778No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT905320.2970818270237026No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC904890.29694072201594823No Hit
AGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATC893520.2932096430899778No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA888810.2916640510282962No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT883290.28985265651014697No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA881940.2894096524160344No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCA861580.28272849437445513No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG851490.27941744895993964No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG847270.27803265097686175No Hit
AGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCAT836820.2746034711372496No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGTAGGCACCATCAAT793590.2604174955902224No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTGTAGGCACCATCAAT748220.24552927651623155No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA740580.24302220149473522No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC737580.2420377479522628No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACACTGTAGGCACCATCA734220.2409351599846937No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCCTGTAGGCACCATCAA725080.23793585819196117No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG723790.23751254316869802No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACC701880.2303227507968413No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAA673860.22112795471014912No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA656160.21531967880956202No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGTAGGCACCATCAATA653000.2142827210781578No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA650960.21361329266927653No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT633200.20778532769783997No Hit
TCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCA617010.20247256008029726No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC599960.19687758244724585No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGCTGTAGGCACCATCAATA578200.18973701275251273No Hit
CAGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCAT566680.18595671114941875No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA557650.18299350598657685No Hit
TAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATCA555110.18216000198728355No Hit
CGACAGAGTGGTTCATTTCCACCTTTCGGGCGCCACTGTAGGCACCATCA554130.1818384138300759No Hit
AGAGAGAACCCACCATGGTGCTGTCTCCTGCCGCTGTAGGCACCATCAAT546680.1793936875329361No Hit
TAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCATC546440.17931493124953832No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGTAGGCACCATCA541800.17779230977051438No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA538880.1768341083225079No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC522550.17147539953964983No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT513480.16849906832957495No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA509470.1671831820944702No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAG506910.1663431150715604No Hit
TTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA487950.1601213686831349No Hit
TCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATC483700.15872672616463235No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA477000.15652811325311064No Hit
TCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGCTGTAGGCACCAT474350.1556585126239267No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCTCTGTAGGCACCA473000.15521550852981414No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAATAG465030.1526001436186458No Hit
TGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATCAA458510.15046059791967245No Hit
TGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCATCA454230.1490561108657452No Hit
GAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA453620.14885593864544247No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA447000.1466835778283867No Hit
CAGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCA444500.1458631998763264No Hit
AGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGCTGTAGGCACCATCA441790.144973910176293No Hit
TGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA438960.14404524233456067No Hit
GAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT435800.14300828460315643No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGA428120.1404880835344271No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA410990.13486685380690974No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA410070.13456495472055155No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC399460.1310832706920075No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCATC395960.12993474155912305No Hit
GCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTCTGTAGGCACCATC389770.1279034857498217No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAA385950.1266499482390735No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG385580.12652853230216857No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCTGTAGGCACCATCAAT382250.1254357888700242No Hit
TGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAAT380600.12489433942166439No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC376920.12368674307623158No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC375070.12307966339170694No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT373610.12260056266770372No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGTAGGCACCATCAATAG370550.12159642005438187No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG367720.12066775221264958No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT364170.1195028155207239No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT360370.11825584103359221No Hit
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACCCCCTGTAGGCACCAT356430.11696292538114514No Hit
TGAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAAT356420.1169596438693369No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATAGAT352850.11578814415379474No Hit
TGCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCCTGTAGGCACCATCA349580.11471508979249985No Hit
CCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA347930.11417364034414003No Hit
CCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCAT347250.1139504975411796No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCTGTAGGCACCATCAATAG346550.11372079171460273No Hit
TGAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAA341580.11208988034590679No Hit
TGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATC339850.11152217880308103No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGA338800.1111776200632157No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG338560.11109886377981791No Hit
TCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGCTGTAGGCACC334220.10967468765504117No Hit
CCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATCA328590.10782719650700133No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT316300.10379421849467274No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT315270.1034562227784239No Hit
TGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACACTGTAGGCACCATC304900.10005329503327764No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)