FastQCFastQC Report
Mon 3 Jul 2023
SRR4045267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24401934
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA15916566.522663326603539No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT9117733.7364784283081827No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA9021913.697211048927515No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG4919572.0160574157769626No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA4107421.683235435355247No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA3783541.5505082506984897No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT2753741.1284925203059726No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC2425190.9938515529137977No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA2355280.9652021843842378No Hit
TGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCAT2274910.9322662703702091No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1974510.8091612738564082No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC1582370.6484608965830332No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1442870.5912932966706654Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA1403740.5752576824443505No Hit
TCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCAT1396760.5723972534308142No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1382810.5666804934395774No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAA1297000.5315152479307582No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA1273920.5220569812212426No Hit
TCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATCA1254290.514012536875151No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC1241220.5086564040374832No Hit
TCGACAGAGTGGTTCATTTCCACCTTTCGGGCGCCACTGTAGGCACCATC1232180.5049517796417283No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1219390.499710391807469No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1096770.4494602763862897No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC991730.4064145079648195No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT983050.40285741285916105No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT979670.40147227674658903No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA945220.38735454329152763No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG930250.38121978364501763No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAAT919750.37691684601720504No Hit
TCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTCTGTAGGCACCATC913030.37416296593540493No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA868670.35598407896685563No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG862470.3534432967485282No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC809420.3317032166384845No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA803620.3293263558535975No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATCAAT772310.3164954056510439No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC768210.31481521095827897No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTGTAGGCACCATCAAT762100.3123113110624756No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA759840.3113851549635369No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT754370.30914352936123835No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG729680.29902547888212466No Hit
AGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATCAA654340.26815087689361017No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCA652200.26727389722470357No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCATCAA641700.262970959596891No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAG640100.26231527386312903No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA629360.2579139833752521No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA614410.2517874198004142No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA599990.24587805212488487No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT587170.24062437018311744No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA581320.2382270192190504No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAATA580760.23799752921223374No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGCTGTAGGCACCATCAATA578040.2368828634648385No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA562710.23060057452823204No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAG562430.23048582952482372No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA559250.2291826541289719No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA554330.227166420497654No Hit
AGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCATCA550320.2255231081274132No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG532070.21804419272669126No Hit
TTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA531020.21761389896391No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG511580.20964731729870265No Hit
GCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATCA491830.2015536965225789No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA490110.20084883435878484No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA482790.19784907212682404No Hit
CGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATC482170.19759499390499133No Hit
AGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATC480210.19679177888113297No Hit
TGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATCAA477280.1955910543811814No Hit
AGAGAGAACCCACCATGGTGCTGTCTCCTGCCGCTGTAGGCACCATCAAT476940.19545172116275703No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT472200.19350925217648732No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGTAGGCACCATCAATA461530.18913664793946253No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGTAGGCACCATCAAT459890.18846457006235653No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC459470.18829245255724403No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA452710.1855221803320999No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCATC445890.18272731989193972No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGTAGGCACCATCA440730.18061273340055753No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA436670.17894893085113664No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA428550.1756213257522949No Hit
TAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATCA414780.16997833040610633No Hit
AGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCAT414100.16969966396925754No Hit
TCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATC399010.1635157278927154No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT396820.16261825804462876No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC395220.16196257231086683No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC387140.15865135935536914No Hit
TGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCATCA387040.15861037899700903No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC385020.1577825757581346No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA382440.15672528251244347No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG380370.155876989094389No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT374900.15363536349209042No Hit
CAGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCAT366040.1500045037413838No Hit
TGAGAGAACCCACCATGGTGCTGTCTCCTGCCGCTGTAGGCACCATCAAT348930.14299276442596723No Hit
AGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGCTGTAGGCACCATCA347940.14258705887820203No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACACTGTAGGCACCATCA347000.1422018435096169No Hit
TCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGCTGTAGGCACCAT346960.14218545136627286No Hit
TAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCATC346700.14207890243453652No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAA342710.14044378613596775No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCCTGTAGGCACCATCAA338540.13873490519235074No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAA327260.1341123207693292No Hit
TTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCAT322150.13201822445712705No Hit
CAGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACCTGTAGGCACCA315980.12948973634630764No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGA314600.12892420740093796No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA311200.1275308752166939No Hit
TGAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAAT303760.12448193655470094No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT297110.12175674272375296No Hit
TAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGCTGTAGGCACCATCAA296600.12154774289611635No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA294470.1206748612630458No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGACTGTAGGCACCATCAA292440.11984295998833534No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGAT289330.11856847084333562No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC287300.11773656956862517No Hit
GCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA286350.11734725616420405No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCTGTAGGCACCATCAAT285480.11699072704647098No Hit
CCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA284280.11649896274614954No Hit
CGACAGAGTGGTTCATTTCCACCTTTCGGGCGCCACTGTAGGCACCATCA282540.11578590451068345No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA279210.11442125857729145No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGA275910.11306890675140749No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT274360.11243371119682563No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATAGAT272050.11148706491870684No Hit
TGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA271520.11126986901939821No Hit
TAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC262220.10745869569190704No Hit
CAGAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGCTGTAGGCACCATC255420.10467203132341887No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGA252390.10343032646510725No Hit
GAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA250020.1024590919719724No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACTGTAGGCACCATCAATA248460.10181979838155451No Hit
TCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGCTGTAGGCACC247930.10160260248224587No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)