FastQCFastQC Report
Mon 3 Jul 2023
SRR4045269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33293679
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA27530098.268863888547733No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA17001555.106539893052973No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT14070074.226048433998537No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA8001492.403306044970278No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG7116082.137366675518197No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC6178971.855898832928617No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC5628531.690570152971079No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA5504641.6533588853307561No Hit
ATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCG4488401.3481237684786953No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT3723501.1183804589453752No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA3620941.0875758128141984No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC3221350.967556033684352No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA2924890.8785121043547036No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2874130.8632659670924322Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2834930.8514919603808279No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2831870.850572866999769No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA2790060.8380149276984378No Hit
ATGTACACGTAGGGATAACAGGGTAATAACGCGACTGTAGGCACCATCAA2536970.7619974950800721No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2452580.7366503413455749No Hit
TGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCAT2348150.7052840270370841No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA1970930.5919832410230182No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1933310.580683798867647No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1785440.5362699628358885No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA1744880.5240874701771469No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1687170.5067538495820784No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1609020.4832809254873876No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1577330.4737626022044605No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1501460.45097449278585283No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1492120.4481691554724247No Hit
TCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCAT1467720.4408404370090791No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAAT1319710.3963845509533506No Hit
TCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATCA1306700.3924769022972799No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC1304980.39196028771707686No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1300290.39055161191408133No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA1205240.3620026492115816No Hit
TCGACAGAGTGGTTCATTTCCACCTTTCGGGCGCCACTGTAGGCACCATC1184150.3556681134578128No Hit
TCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTCTGTAGGCACCATC1169140.351159750173599No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAA1114460.33473621224016725No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA1099230.33016177034685773No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG957030.2874509602858849No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC936320.2812305603114633No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA928890.2789989054679118No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG926130.2781699192810743No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG913030.2742352384667372No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC903900.271492976189264No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT887950.266702277029823No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT854150.256550199814205No Hit
CGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATC785540.2359426844957567No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT777960.23366597605509443No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAATA774110.23250960039591897No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA768700.23088466732679194No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA740210.22232748744889383No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC728720.21887638191021183No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC725250.2178341420303836No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA722930.2171373130617376No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAG715990.21505283330208116No Hit
GCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTCTGTAGGCACCATCA685890.20601207814852784No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA680760.20447124512734086No Hit
TGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTGTAGGCACCAT676430.2031706979574111No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAG669860.20119735040396106No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTGTAGGCACCATC660520.1983920130905329No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCATC657670.19753599474542902No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA636640.19121948043050455No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTCTGTAGGCACCATCAA633280.19021027985522415No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT626020.1880296857550648No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA607130.18235593609225342No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT600400.18033453136855196No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCA593910.17838521240022767No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA584600.17558888580622165No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG580630.17439646726935765No Hit
TTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGCTGTAGGCACCAT535130.16073020947910263No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC533750.1603157163856839No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA522280.15687061799328333No Hit
CGACAGAGTGGTTCATTTCCACCTTTCGGGCGCCACTGTAGGCACCATCA521550.15665135715401113No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT502810.1510226610883105No Hit
GAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA492580.14795000576535863No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA489250.1469498159095004No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC484210.14543601504657988No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCTGTAGGCACCATCAAT483860.14533088998665483No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGTAGGCACCATCAATAGAT481320.1445679824089131No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGA481300.1445619752626317No Hit
TTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA480520.1443276965576559No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCCTGTAGGCACCATCAA478950.14385613557456356No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG476810.14321337092245048No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA466660.14016474418462435No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC460050.1381793823386115No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA452480.13590567747108995No Hit
ACGTAGGGATAACAGGGTAATAACGCGACTGTAGGCACCATCAATAGATC438500.13170668222036983No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGTAGGCACCATCA428810.1287962198470166No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT411790.12368413836151902No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGAT410470.12328766670694459No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGCTGTAGGCACCATCAAT395640.11883336773926365No Hit
TGAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAAT386780.11617220193658982No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGTAGGCACCATCAAT386260.11601601613327261No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGAT385890.11590488392706616No Hit
TAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC385790.115874848195659No Hit
TATGTACACGTAGGGATAACAGGGTAATAACGCGACTGTAGGCACCATCA381560.1146043367571364No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGA379990.11413277577404408No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGTAGGCACCATCAATA378970.1138264113136911No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAA377940.11351704328019742No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA374880.11259794989913852No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC362760.10895761925259145No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA358520.10768410424092814No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC352130.10576482100401101No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGTAGGCACCATCAATAG350920.10540138865398445No Hit
CCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA347910.1044973131386291No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA343670.10322379812696579No Hit
TATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATC342830.10297149798314569No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGACTGTAGGCACCATCAA336600.10110027191648No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC336190.10097712541771067No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA333050.10003400345152604No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)