FastQCFastQC Report
Mon 3 Jul 2023
SRR4045270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38866550
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT22830565.874089673511026No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA15390003.95970313804544No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA14927853.8407962631105663No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC9967302.564493118118279No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC8849622.2769245019174584No Hit
ATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCG8244982.1213562819442426No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC6174801.588718319480376No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA5036941.295957577917258No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA4342721.1173412613159646No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT4223621.0866979446336245No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2394550.6160953313324697No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA2355930.6061587663427805No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC2274160.5851201097087341No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2254570.5800797858312611Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2231680.5741904028013807No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC2018190.5192614214536664No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC1949860.5016807511857884No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1814150.4667638367696644No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1666710.42882890300271054No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1567640.40333911808483125No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA1564020.40240772592370566No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC1348020.3468329450388573No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1338970.34450446463604306No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1197030.3079846294564349No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1108170.2851217820979737No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1097130.2822812932971926No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1063260.2735668589056657No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1046860.2693472922088531No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC942950.24261222053410966No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA938350.24142868353378416No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC896440.23064563229821017No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA882480.2270538547928746No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT803610.20676134105033764No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA799940.20581708435659968No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC772890.1988573722133814No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT743930.19140623492437586No Hit
TGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCAT733320.1886763811040599No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT698160.1796300417711374No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA672340.17298679713018006No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCCTGTAGGC667110.17164116701894044No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC663770.17078181624044325No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT649840.16719775745467502No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG645860.16617374065874124No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA635440.1634927720623518No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA627050.16133410349001906No Hit
ATGTACTCGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCG623420.1604001384223709No Hit
TGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA610040.15695758949533725No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA548500.1411239227562004No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA538880.13864878668160666No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA532750.13707159498334687No Hit
ATGTACACGTAGGGATAACAGGGTAATAACGCGACTGTAGGCACCATCAA530990.13661876343539625No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC527050.13560503826555226No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA524090.13484345793490804No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC517150.13305786080833004No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC495320.12744120586982893No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCA488000.125557838295398No Hit
TATGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATC476740.12266074555112301No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC423130.10886739368428636No Hit
CGTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT414170.10656206944017414No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT403700.10386823631117247No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG398100.10242740865860232No Hit
TGTACTAGGGATAACAGGGTAATAACCTGTAGGCACCATCAATAGATCGG396870.10211094115634138No Hit
CCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA393500.10124387165827685No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT389070.10010407406883295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)