FastQCFastQC Report
Sun 2 Jul 2023
SRR4045273.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045273.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22581683
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA293934813.016514313835687No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC15272606.76326915048803No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC10188504.511842629267269No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA8605103.810654856858986No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA7921943.508126475781278No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT7703273.411291355033192No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC6500202.8785276987547825No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3158761.3988151370294233Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT2444771.082634097733105No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2244780.9940711682118644No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1594550.7061254026106025No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA1439470.6374502733033671No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1297020.5743681726468306No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1263850.5596792763409176No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC1206430.5342515878909468No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1205720.5339371737704405No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA1159120.5133009793822719No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1074350.4757617047409619No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC1011300.4478408451664121No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC943090.4176349477583225No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG865910.3834568043489053No Hit
TGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCAT858850.3803303766154188No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA789140.3494602240231607No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC779880.34535955535289375No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA775560.3434465004224885No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC748610.33151204894692743No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA741360.3283014822234463No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC695350.30792656154105075No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA629690.2788498979460477No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT628270.2782210697050348No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG566920.25105303267254264No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC561630.24871042605637497No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC557430.24685051154070314No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA553990.24532715298501004No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT552420.2446318992255803No Hit
TGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA520720.23059397300015239No Hit
CCATTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC506290.22420383812845127No Hit
GCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC501140.22192322866280606No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA493760.21865509315669696No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT448080.1984263086148185No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC430310.19055709886636882No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA419070.1855796133529994No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT395860.17530137146996527No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC391170.17322446692746507No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCCTGTAGGC375500.16628521443685132No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA360790.15977108526410544No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA355090.15724691556426507No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC346800.15357579857976042No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT333180.14754436150751032No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT327920.14521503999502605No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCCCACGTCTGAACTCCAGTCA319440.14145978402052672Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA318820.1411852252110704No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA305850.13544163205195997No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA298800.13231963268636798Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG294290.13032243876596797No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA285350.12636347786832364No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA284080.12580107514572764No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCTGTAGG266060.11782115619991655No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG258610.11452202211854624No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA244260.10816731419000081No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)