FastQCFastQC Report
Mon 3 Jul 2023
SRR4045274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29478595
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA7370392.5002514536394966No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT6524672.2133585403239198No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC5513751.8704249642834063No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA5161021.7507686509482558No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4701341.594831775394994Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4346731.4745377111765334No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT4333481.4700429243659678No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT3409541.1566155035543586No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT3158861.0715775293903933No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCA3131131.0621707038615646No Hit
TCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCATCA2916330.9893042731514171No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCATCAATA2785800.9450246865564659No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA2521760.8554546103706774No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2337400.7929143163030666No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2336640.7926565021161964No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2310760.7838772505948808No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCAC1892110.6418589488406757No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA1802980.6116234508462836No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACC1745650.5921754411972483No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG1724600.5850346666793312No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACC1717770.5827177312894322No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGC1634640.5545176084545413No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA1508890.511859537403326No Hit
TTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCAT1480450.5022118591472897No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1438660.488035471161363No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCAA1423730.48297077930613724No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1327090.45018767007043586No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC1301030.4413473572943351No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1204110.40846926388452365No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1100330.3732640582090157No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1059430.35938958420508166No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT1038150.352170786972717No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1020960.34633943714074567No Hit
TCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCATCAA965910.32766487005232103No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG959350.32543952654459957No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCTGTAGGCACCATC946640.32112792349838926No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG912770.3096382307230043No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCAC892100.3026263632985222No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTCTGTAGGCACCAT881200.2989287650920948No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCA848160.2877206325471075No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA824990.2798606921394999No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCAC792280.26876450522828516No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA763190.2588963279966362No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC742520.25188446057215413No Hit
CCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCATCAA735470.24949289475974007No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG720520.24442141832064926No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC616640.20918228972581632No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATC591030.20049463008667817No Hit
TCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGCTGTAGGCACCATCAA576600.19559955282807745No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCACTGTAGGC563670.1912133193593521No Hit
TGGTTAAATTTTTTGAAGTCTTGCCTAGGCAAGGCTCTGTAGGCACCATC561020.19031436199723903No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAA556110.1886487466583804No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC550970.1869051086050743No Hit
CAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCAC530720.1800357174417573No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA497910.16890560761121756No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT497700.16883436948063502No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCACTGTAGGCACCATCAATA491560.16675150223407867No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAATA486320.16497394126144752No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT485320.16463471206819727No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA484660.16441082080065214No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA480850.1631183575743688No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG478630.16236526876535332No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT476240.16155451099348528No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACCCGTCTGAACTCCAGTCA471200.15984479585950417Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCA464520.15757874484859266No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT460540.1562286126594568No Hit
GCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTCTGTAGGCACCAT452340.153446933274805No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCCCACGTCTGAACTCCAGTCA447370.1517609641843514Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCA441250.14968488152166004No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCA421560.14300545870656317No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACCA416720.14136358941123212No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG406140.13777454454664476No Hit
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCAT401110.13606822170459618No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGCTGTAGGCACCATCAATAG398790.13528120997625567No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCCTGTAGGCACCATCAATAGA398700.13525067934886315No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCAA395800.1342669146884375No Hit
TTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCATC390150.13235026974657374No Hit
TTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGCTGTAGGCACCATC385490.1307694617060277No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA381940.12956519806998942No Hit
TAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCAT381780.1295109213990694No Hit
CCGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGCTGTAGGCACCAT377230.12796742856978088No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC374930.12718720142530537No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTCGGCACCATCAATAGATC368080.12486348145154136No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCCTGTAGGCA366980.12449032933896612No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGTAGGCACCATCAAT366370.12428339953108349No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCTGTAGGCACCATCAATAGATCG363220.1232148275723453No Hit
AAAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGC361950.12278400649691751No Hit
GCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTCTGTAGGCACCA361900.122767045037255No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGCTGTAGGCACCA336430.11412687748517188No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCG335130.11368587953394658No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCA333770.11322452783112627No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC333730.11321095866339626No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTGTAGGCACCATCAATAG328050.11128413684573502No Hit
AAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCATC327280.11102293036693235No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTCTGTAGGCACCATC326240.1106701320059521No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA325490.11041571011101445No Hit
TCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTCTGTAGGCACC319890.10851602662881321No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG316660.10742031633461499No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTCTGTAGGCACCATCA315650.10707769484943226No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAA313520.10635513666780931No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATA312530.10601929976649159No Hit
TGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGATC310860.1054527870137637No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAG308300.10458436027904316No Hit
TCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCTGTAGGCACCA307910.10445206089367556No Hit
TCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACCTGTAGGCACCATC307440.10429262317284795No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA306740.1040551627375728No Hit
TTGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT297330.10086301602908823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)