FastQCFastQC Report
Sun 2 Jul 2023
SRR4045275.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045275.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26066426
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA7795122.9904828533071623No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT6707962.573409948874464No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4655811.786132859180618No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA4382331.6812162894905498No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC3668341.407304553374521No Hit
TCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCATCA3640761.3967238930262245No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2987061.145941526467802No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGAT2924641.1219950138158563No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2654741.018451858340687No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2654651.0184173311676867No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCA2612881.002392886543019No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2083330.799238836962152Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCATCAATA1930130.7404659158106294No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1884350.722903093811173No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA1877810.7203941192398222No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCAC1751880.6720829315073727No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG1721530.6604396015011801No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1639230.6288664199687367No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCAT1635960.6276119326830613No Hit
TTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCAT1624560.6232384907696974No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACC1623760.6229315825652508No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCAA1547570.5937024124442684No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA1419360.5445165363291461No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACC1385480.5315189738708329No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA1357090.5206275689655345No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGC1276100.48955694961787244No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1154150.4427726302025448No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCTGTAGGCACCATC1149890.44113834401386676No Hit
TCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCATCAA1123190.4308952826904616No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1055610.40496921211983566No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1009280.38719539072982234No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTCTGTAGGCACCAT982150.37678736624652726No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC975400.3741978282715091No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA927860.3559598082222703No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA917330.35192012898124203No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCAC863840.33139947916143164No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCA841220.3227216496807042No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCAT841150.32269479521281513No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC825210.3165796492392168No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG813770.3121908619156305No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCAC750280.2878338595402377No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACC740980.284266051663546No Hit
CCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCATCAA711890.27310610207935676No Hit
TCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGCTGTAGGCACCATCAA693890.26620066747930843No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGCTGTAGGCACCATCAATAG692240.2655676693076373No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAA677260.2598208131793749No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCA617930.23705973346710438No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG611160.23446252278697507No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA552140.21182037000392764No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT530270.20343026696486888No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT514110.1972307212350477No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC510680.19591485230848293No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA505990.19411560295991478No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCACTGTAGGC500610.19205164528501145No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCACTGTAGGCACCATCAATA494730.189795869982329No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCAA472930.18143262141115932No Hit
TTTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGCTGTAGGCACCATC465640.17863592039813975No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAG447670.17174199485575814No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT444410.17049134392263826No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGTAGGCACCATCAAT422220.16197847760180087No Hit
TGGTTAAATTTTTTGAAGTCTTGCCTAGGCAAGGCTCTGTAGGCACCATC421920.16186338702513342No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG419760.1610347348731276No Hit
TAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCA410490.1574784360541027No Hit
GCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTCTGTAGGCACCAT408570.15674185636343088No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG404960.15535693309086562No Hit
TTCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACTGTAGGCACCATC395730.15181597968206306No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT392840.15070727379349974No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA388620.14908833301504396No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAATA385760.1479911361841474No Hit
TCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCTGTAGGCACCA383570.14715097497447482No Hit
CCGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGCTGTAGGCACCAT377810.14494123590245936No Hit
CAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCAC375770.14415861998112053No Hit
TCCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACCTGTAGGCACCATC372610.1429463325735565No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCA370470.14212535312666186No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCCTGTAGGCA370250.14204095337043904No Hit
GCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACCA363190.13933248846619786No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCCTGTAGGCACCATCAATAGATC362510.13907161649241825No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT357650.1372071491504052No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCTGTAGGCACCATCAATAG355890.1365319511006227No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT348720.1337812863182701No Hit
TCACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTCTGTAGGCACC347750.13340916012037862No Hit
TAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCAT346580.13296030687137547No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGA343300.13170198323314442No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGCTGTAGGCACCA342340.1313336933878085No Hit
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCCTGTAGGCACCATCAATAG334410.12829146581123166No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCCTGTAGGCACCATCAATAGA326510.12526074729232156No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA324000.12429782280087036No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCG323280.12402160541686842No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC323180.12398324189131259No Hit
CCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCAT321160.12320829867508494No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA317400.12176583011418596No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA311690.1195752728049484No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATC311120.11935660070928021No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC310770.11922232836983483No Hit
TCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATC302960.11622613702392495No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAA302640.11610337374214633No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCA301740.1157581020121439No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT299230.1147951775206927No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC296760.11384759843946386No Hit
TCGACTGCACAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT295330.11329900002401558No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCC293690.11266983820490005No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCTGTAGGCACCATCAATAGAT289220.1109549886125547No Hit
TAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACC283380.10871455872009457No Hit
CGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCCTGTAGGCACCATCA279630.10727592651175116No Hit
CAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCA279130.10708410888397205No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA278060.10667361916052473No Hit
AAAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGC271630.10420684446728523No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTCTGTAGGCACCATCA270420.10374264580805977No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCCTGTAGGCACC265850.1019894326901586No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAGA263750.1011837986534863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)