FastQCFastQC Report
Mon 3 Jul 2023
SRR4045276.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045276.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66083789
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG37614935.692005644531067No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG11919361.803673817795163No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA10538961.5947874901664612No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG9755421.4762198335812735No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA8818361.334421063537988No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC7604121.1506785726224023No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG7240841.0957059378057152No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6947141.0512623602741664Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6301470.9535576115346533No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA6285980.9512136176090024No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG4772570.7221998121203371No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA4535240.6862863144847823No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAG3695590.559227921994606No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT3661200.5540239225689677No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3169730.4796531869563351No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT3104950.46985048027436804No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2799230.4235879997740445No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA2744410.415292470593658No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2604630.3941405357371382No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA2487950.3764841631583807No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAAT2318760.35088181762701287No Hit
AAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAATAGATCGGA2181560.3301202962196977No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG2147410.32495261432421796No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAA2116120.3202177163297946No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA2097330.31737435636446326No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2082530.3151347753380182No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC2015980.3050642268711317No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1881570.28472489675190993No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1873680.28353095794794697No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1857080.2810189954453126No Hit
CAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCGG1845680.2792939127627806No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1824460.2760828378045938No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1759980.26632552803532494No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA1672070.25302271938432586No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1598540.24189593608199433No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1584670.23979708548491369No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1514700.22920901221326762No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA1464480.22160956902758708No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1459540.22086203319848988No Hit
CAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGGA1458080.2206411015566919No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCCTGTAGGCACCATCAATAG1383220.20931305860806496No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG1358340.20554814131496002No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1337160.20234311927846632No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGAT1326010.20065586735651614No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC1319480.19966772789011838No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC1298840.19654442029648148No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAG1298240.19645362647108508No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATAGAT1294240.19584833430177556No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1284220.19433207741765532No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA1237920.1873258205578981No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1229760.18609102453250675No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1177500.17818288234047838No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCG1140500.17258392977436568No Hit
CAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCA1137320.17210272249976466No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1132690.17140209681378893No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1004040.15193438741837276No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG971330.1469846107038445No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAAT962210.14560454455781888No Hit
CGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGAT959430.14518386650014878No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA940720.14235261237820368No Hit
AAAAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAATAGATCG932210.14106485328799775No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAATA923730.13978163388906167No Hit
TCAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGG917300.13880862672689667No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC868100.13136353304439005No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAG864150.13076580702719695No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT838750.12692220175208174No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT827280.12518652645658682No Hit
CCGGGGAGGTAGTGACGAAAAATAACAACTGTAGGCACCATCAATAGATC818400.12384277784071974No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT810440.1226382464237939No Hit
AAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAATAGATCGGAA806990.12211618192776445No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATC804400.12172425524813657No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC798090.12076940685105088No Hit
GTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGATC781590.11827257665264926No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCATCATCAATAGATCGG776540.11750839528889603No Hit
CGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAA766360.11596792671800342No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATAGATCG752640.11389177457727188No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA742600.11237249123230508No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAA739300.11187312519262478No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATAG738720.1117853578280749No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT737440.11159166433389588No Hit
TGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGATCG735770.11133895485320915No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA735250.11126026687119893No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG715940.10833821892385742No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC715040.10820202818576277No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA710440.10750594219105687No Hit
ATGCAGCTCAAAACGCTTAGCCTAGCCACACCTGTAGGCACCATCAATAG704300.10657681871116681No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA699740.10588678563815401No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTCTGTAGGCACCATCAATA697300.10551755741487522No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG672590.10177836503896592No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG665520.10070851112971141No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT662700.10028178015034821No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)