FastQCFastQC Report
Mon 3 Jul 2023
SRR4045277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80489523
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA22499102.7952830581441015Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA13791661.7134726963160163No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG5211550.6474817846789824No Hit
TTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAGATCGG5116070.6356193712317068No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT5057680.6283650109344046No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG4756930.5909998994527524No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA4749040.5900196476502911No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA4623240.5743902843106673No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCCTGTAGGCACCATCAAT3825220.47524446131951853No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCTGTAGGCACCATCAA3734270.46394485404019603No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT3731660.4636205882348191No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCCTGTAGGCACCATCAATA3383990.4204261466427128No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3349350.41612248093456833No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAAT2521620.31328549431209823No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCTGTAGGCACCATCAATAG2441350.3033127677996054No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG2246880.2791518593047197No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGACTGTAGGCACCATCAAT2215560.2752606696401965No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA1905280.23671155312971603No Hit
ACGAGCGCACGTGTTAGGACCCGAAAGATCTGTAGGCACCATCAATAGAT1870730.2324190690010674No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA1789710.22235316265944324No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAA1721550.21388497978799054No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1691850.21019505855439097No Hit
TCGGGACATGTGGCGTACGGAAGACCCGCTGTAGGCACCATCAATAGATC1643260.20415824802440438No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCTGTAGGCACCATCAATAGA1534630.19066208157302658No Hit
TCGGGGCGCGGGACATGTGGCGTACGGAAGCTGTAGGCACCATCAATAGA1464180.18190938962329295No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1459840.18137018901205315No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG1406520.17474572435967847No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1375630.17090795779719056No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA1368860.17006685453956535No Hit
AACGCGCCCGATCTCGTCTGATCTCGGAAGCCTGTAGGCACCATCAATAG1366940.1698283141769892No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCA1314770.16334672526261584No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAA1301410.16168688190635694No Hit
ACCTCTAGCATCACCAGTATTAGAGGCTGTAGGCACCATCAATAGATCGG1283390.15944808121176218No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAG1272480.15809262529733217No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGACTGTAGGCACCATCAA1265930.15727885478958548No Hit
TCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAA1264090.15705025360878336No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1175880.14609106330522048No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1153260.14328075965862042No Hit
CCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTCTGTAGGCACCATCAAT1137540.14132771044002831No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCCTGTAGGCACCATCAATA1111540.13809747636347652No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAGA1084490.1347367905261409No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1060190.13171776406228672No Hit
CTCCATCTAAGGCTAAATACCGGCACGCTGTAGGCACCATCAATAGATCG1036440.12876706947312883No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGCTGTAGGCACCATCAATA1020270.12675811235705797No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA1016130.12624375970025314No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGAT985050.122382387581052No Hit
TCCATCTAAGGCTAAATACCGGCACGCTGTAGGCACCATCAATAGATCGG979290.12166676649332361No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG969880.12049767023715621No Hit
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTCTGTAGGCACCATCAATAGA962960.11963793101370473No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAATAGAT953020.11840298767828454No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG940660.11686738409420068No Hit
TTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGCTGTAGGCACCATCAAT909900.11304576870209557No Hit
TGGCGGGGCGCGGGACATGTGGCGTCTGTAGGCACCATCAATAGATCGGA894770.11116602094908676No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCTGTAGGCACCATCAATA886410.11012737645370319No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCCTGTAGGCACCATCAAT877980.10908003517426734No Hit
TCCATCTAAGGCTAAATACCGGCACGAGCTGTAGGCACCATCAATAGATC872360.10838180765464345No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCA870130.10810475296269305No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG851730.10581874115467177No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCCTGTAGGCACCATCAATAGATC817430.10155731696906689No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA812560.10095226927857431No Hit
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTCTGTAGGCACCATCAATA810670.10071745610916343No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA808040.10039070550834299No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)