FastQCFastQC Report
Thu 6 Jul 2023
SRR4045278.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045278.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28296200
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA443640215.678437387352364Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA12215074.3168588008283795No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT9572063.382807585470841No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA3799651.3428128158551325No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAA3564221.2596108311363363No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG3544661.2526982421667927No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3359781.1873608470395318No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA3349021.1835582162975948No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATAG2176510.7691880888599882No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1852730.6547628303447106No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAAT1811370.6401460266749599No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA1438770.5084675680833469No Hit
TCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAA1423970.5032371837914632No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1351540.4776401071521971No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCAA1348950.4767247899011175No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1332290.4708370735293078No Hit
AGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCTGTAGGCACCATCAA1158890.40955676027169724No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1156660.40876866858447425Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1125610.3977954637018399No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1065910.37669722436228187No Hit
CAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT1063860.3759727454569871No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA1023860.3618365716951393No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA974000.3442158311009959No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGAT880430.3111477866285932No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA848770.29995900509609064Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT838580.2963578148302599No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACCTGTAGGCACCATCAATAGATC833650.2946155314141121No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA768580.2716195107470261Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCATCTAAGGCTAAATACCGGCACGAGACCGACTGTAGGCACCATCAAT758350.2680041843074335No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA710430.2510690481407397No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA704720.24905110933623595No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC700570.24758448130844424Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCATCTAAGGCTAAATACCGGCACGAGACCGATCTGTAGGCACCATCAA670620.2370000212042606No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA641350.22665587605402845Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCGGAGGAGAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAATA636950.22510089694022517No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG635330.22452838190287036No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG606710.2144139495762682No Hit
TAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT593370.2096995356266919No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCTGTAGGCACCATCAATAGA585880.2070525370897859No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGCTGTAGGCACCATCAA574650.2030838063061471No Hit
ATGGGGATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC574590.20306260204550433No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG570740.20170199532092647No Hit
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGCTGTAGGCACCATCAA568750.20099872067627453No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA561730.19851782218107022Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACGCGCCCGATCTCGTCTGATCTCGGAAGCCTGTAGGCACCATCAATAG555630.19636205568238843No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG538450.1902905690516748No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA536000.1894247284087616No Hit
GTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAATA529250.18703924908644978No Hit
TCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCCTGTAGGCACCATCA522150.18453007824372178No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA497400.17578332072857838No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA496490.17546172277549635No Hit
TATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCAAT495450.1750941822576883No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA491260.17361341805613473No Hit
AACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAATAGAT479800.16956340427336533No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA454800.16072829567221042No Hit
AGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCCTGTAGGCACCATCAAT450970.15937475703451345No Hit
CAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCTGTAGGCACCATCAAT449890.1589930803429436No Hit
TCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGAG447070.15799648009273332No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA433800.15330680444724026No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG412990.1459524600476389No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCTGTAGGCACCATCAATAGAT399440.14116383118581294No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCTGTAGGCACCATCAATAG395520.13977848615715183No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG392130.13858044543083523No Hit
CCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGCTGTAGGCACCATCAA388420.13726931531442385No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA387500.13694418331790134No Hit
ACGTGGCGACCCGCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA376950.13321576748821395No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT369900.13072426686268826No Hit
ATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAG369720.13066065408075994No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCTGTAGGCACCATCAATAGAT367020.12970646235183522No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGC351620.1242640354535238No Hit
TGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCCTGTAGGCACCATCAAT341930.12083954735971614No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA336640.11897003837971178No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG334450.11819608286625059No Hit
TTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGA333080.1177119189149073No Hit
AAACTAACCAGGATTCCCTCTGTAGGCACCATCAATAGATCGGAAGAGCA331480.11714647196443338No Hit
ATTCCCCATGAACGTACACTGACTGGCTCAGCGCTGTAGGCACCATCAAT323480.1143192372120638No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCCTGTAGGCACCATCAATA316200.11174645358740751No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCATCATCAATA315290.11142485563432547No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC314730.11122694920165958No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA299110.10570677334765799No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACCCTGTAGGCACCATCAATAGAT288360.10190767664916137No Hit
CTGTAGGCACCATCAATAGATCGGAAGGGCACACGTCTGAACTCCAGTCA285580.10092521257271295Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTGTAGGCACCATCAATAGATC283480.10018306345021594No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)