FastQCFastQC Report
Sun 2 Jul 2023
SRR4045280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27466130
Total Bases738.1 Mbp
Sequences flagged as poor quality0
Sequence length3-34
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCGCGGGGGCGCGCCGGC9691583.5285568079667575No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC5382841.9598101370669985No Hit
TCCCCCGCGGGGGCGCGCCGGC4971551.8100657063809134No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4953121.8033556238174069No Hit
TTCCCCCGCGGGGGCGCGCCGGC4687881.7067857757900367No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT4049421.4743322047918654No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT4012101.4607445606643528No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC4004581.4580066430909633No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTT3925451.4291966141571455No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC3668621.3356887191606537No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT3543951.2902982691773468No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3228701.175520541117369No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3163231.1516839103288303No Hit
TCCCCCCCGCGGGGGCGCGCCGGC2906191.0580995575277623No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT2583150.9404856090027972No Hit
CCCCCCGCGGGGGCGCGCCGGCG2206770.8034513781155189No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC2152400.7836560884259997No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT1663500.6056550376773139No Hit
TCTCCCCCGCGGGGGCGCGCCGGC1598130.5818548153671449No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT1513630.5510896511448828No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1502360.5469864156326355No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGT1500320.5462436826738969No Hit
TCGCCCCAGCTGCGGCGGGCGTCGCG1448260.5272894288347139No Hit
ACGATGCCGACCGGCGATGCGGCGGCG1402420.5105997823501163No Hit
TCCCCCGCGGGGGCGCGCCGGCG1400870.5100354509353884No Hit
TCGCGGCGCCTCGCCTCGGCCGGCG1387510.5051712782252178No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1369540.4986286746622113No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTA1354230.49305453662383447No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGT1353390.4927487054055304No Hit
TTCCCCCGCGGGGGCGCGCCGGCG1285870.4681657008104163No Hit
TCACCCCCGCGGGGAATCCCCC1277790.4652238957581574No Hit
TCCCCGCCCCTTGCCTCTCGGC1248660.4546181060091101No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1234860.44959373599411345No Hit
TCCCCGCGGGGGCGCGCCGGC1138800.41461975167233245No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT1120630.4080043311525869No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG1112580.4050734486438388No Hit
TCGGGTCGCGCCGTCGGGCCCGGGGG1102460.40138891063284127No Hit
ACCCCCGCGGGGAATCCCCC1098300.39987431793266837No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1069180.389272169031458No Hit
GTGGGGGGCCCAAGTCCTTCTGCTCGAGGCCC1068320.38895905611748No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC1058180.3852672364108085No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGT1053590.3835960872536466No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1051100.382689516142245No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG1036950.377537716452955No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGT958950.34913910332471304No Hit
CCCCCCCGCGGGGGCGCGCCGGC945250.3441511417880859No Hit
TCGCCCCAGCTGCGGCGGGCGTCGC938690.341762745607044No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC932340.3394508072305782No Hit
ACCCCCCCGCGGGGGCGCGCCGGC896140.3262709380608043No Hit
TCGATGCCGACCGGCGATGCGGCGGCG882770.32140312450279673No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC871340.3172416354251582No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGC868920.31636055024861526No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGT854430.3110849617328688No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC851560.310040038403663No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGG847990.3087402557258703No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG811990.2956332035128356No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGG808880.2945008998355429No Hit
CCCCCGCGGGGGCGCGCCGGC802070.2920214824585772No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC797150.29023018532279576No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC796410.28996076258286113No Hit
CCGCGAGGGGGGCCCGGGC794460.2892507972546551No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA791060.2880129089900907No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG780040.2840006946737673No Hit
TCCCCCCCGCGGGGGCGCGCCGGCG778150.283312574432583No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCT739670.2693025919559836No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC713220.25967254942724No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC710240.2585875767718277No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT705350.25680720217955716No Hit
AAAACGCTGCCGACCGGCGATGCGGCGGCGTT679720.2474757091734438No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC669740.24384214303216362No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG658550.23976803430261198No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGC651900.2373468704910375No Hit
GGGTCGCGCCGTCGGGCCCGGGGG644930.23480919954868051No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC632020.23010886499117275No Hit
GTCTAGGGGTATGATTCTCGCTT631380.22987585072960767No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC620990.2260930098270124No Hit
ACCCCTCCTCCCCGCGC611260.22255046488165606No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCC607900.2213271400084395No Hit
TAAACGCTGCCGACCGGCGATGCGGCGGCGTT604850.2202166814181685No Hit
TCGCGGCGCCTCGCCTCGGCCGG590620.2150357549461828No Hit
CCCCCGCCCCTTGCCTCTCGGC587620.21394350059509656No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC566210.20614844537617788No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG548430.19967501792207348No Hit
ACCGCCCGTCCCCGCCCCTT538010.1958812544759673No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC535190.19485453538594624No Hit
TTCCCCGCGAGGGGCCCGGGGCGGG532560.19389699240482733No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC531700.1935838794908493No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC525230.19122825094034No Hit
ACGATGCCGACCGGCGATGCGGCGGCGT520440.18948428482643895No Hit
TCGCGGCGCCTCGCCTCGGCCGGC508410.18510434487858318No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCC503360.18326571672092137No Hit
TTGGTCTAGGGGTATGATTCTCGCTT501730.18267225852349786No Hit
AACGATGCCGACCGGCGATGCGGCGGCG499490.1818567086080201No Hit
ACCCCCGCGGGGAATCCCCCG492790.17941734055726088No Hit
TCCCCGCCCCTTGCCTCTCGGCG471870.17180068688235292No Hit
CTCCCCCCCGCGGGGGCGCGCCGGC462160.1682654236326705No Hit
TCACCGCCCGTCCCCGCCCCTT457840.16669257736710633No Hit
TCTCCCCCGCGGGGGCGCGCCGGCG445190.1620869048533594No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC434350.15814022579810116No Hit
TCCCCGCGAGGGGCCCGGGGCGGG431490.15709894331673227No Hit
TCGCCCGGGGCACCCCCCTCGCGG423250.15409888469908212No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC411810.14993375477360663No Hit
TTACCCTACGCCGGCAGGCGCGGGT410780.14955874744640035No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC410450.14943859946778087No Hit
TCGCGCGGGTCGGGGGGCGGGGCGGACTGT409720.1491728175756832No Hit
TAACGATGCCGACCGGCGATGCGGCGGCG405060.14747618248366262No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCC399460.14543730769496832No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTT396880.14449796895303416No Hit
ACCCCTCCTCCCCGCG395250.14390451075561062No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC365690.13314216454957434No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT365630.1331203194625526No Hit
TCCCCGCGGGGGCGCGCCGGCG356450.12977802114822876No Hit
TCGCGCCGTCGGGCCCGGGGG353180.12858746390554476No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCC352620.12838357642667533No Hit
AGGGCGCCCTGGAATGGGT343320.12499758793830802No Hit
CGCGGCGCCTCGCCTCGGCCGGCG340450.12395266460910219No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCC338200.12313347384578753No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT337960.12304609349770063No Hit
TTCCCCGCGAGGGGCCCGGGGCGGGG333510.12142591621025604No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC332190.12094532429577812No Hit
ACCCCCCCCGCGGGGGCGCGCCGGC331090.12054483103371316No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGT321670.11711515237130239No Hit
TTACCCTACGCCGGCAGGCGCGGGTA320820.11680568030516131No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGC319640.11637606026040073No Hit
ACCTCTAGCATCACCAGTATTAGAGGC312330.11371460049158727No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG308590.11235292340056645No Hit
TCCCGCGAGGGGGGCCCGGGC299810.10915625899972074No Hit
TTTGGTCTAGGGGTATGATTCTCGCTT298520.10868658962875366No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC298190.10856644165013417No Hit
TCACCCCCGCGGGGAATCCCC297720.10839532180179734No Hit
ACCCCTCCTCCCCGCGCCCCCGCCC294700.1072957857550372No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGG291170.10601056646859242No Hit
TTCGCGCCGTCGGGCCCGGGGG289480.10539526318414716No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT289190.10528967859687549No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT286500.10431029052873485No Hit
CTCCCCCGCGGGGGCGCGCCGGC278130.10126290088920425No Hit
TCGCTTCTGGCGCCAAGCGCCCGG276000.10048740029993304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)