FastQCFastQC Report
Mon 3 Jul 2023
SRR4045283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107866178
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA86402868.010190182134757No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA32763783.037447011425583No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC29143592.701828371076613No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA22211432.0591653854649414No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT17082331.5836595230063681No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT15134801.4031089522797406No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA11642851.0793791173355562No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA9421650.8734572944635157No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC7708670.7146512598230745No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC7225640.6698707726531296No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA6025080.5585698976003395No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA5991830.5554873743649283No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC5929630.5497209699967306No Hit
TGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCAT5405330.5011144457162466No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4826090.44741457326874046No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA4352730.40353056729237224No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA4205400.3898719763668645No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATAGATCG4194690.388879079408932No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA3863980.3582197934184708No Hit
TATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCAT3730640.34585817993847895No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT3594720.33325738119691234No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATC3445670.3194393334303548No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA3192050.2959268659727612No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGGCTGTAGGC3147670.2918125086438123No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2866920.2657848876410547No Hit
TCTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA2697380.25006726390175793No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG2634670.2442535787260396No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGA2397620.2222772739755366No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCTGTAGGCACCATCAATAGATCG2355700.21839097701227533No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCA2343980.217304445513959No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC2295510.21281091465018814No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA2159810.20023051155108137No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA2146230.19897154416651347No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGAT2100890.19476818767046702No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2063710.19132132409475006No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAAT2011600.18649033805573423No Hit
TCCTCCTCGGCGCGCGGCGGCGGCGGCGGGCGGCGGAGGGGCCTGTAGGC2008230.1861779138962354No Hit
TGCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1952620.18102245172717624No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA1890630.17527551592678103No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAG1762790.1634237935082858No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA1727460.16014843874416315No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA1721250.15957272538200065No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTTTAGGCA1665930.15444414837800222No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT1663260.15419661944451207No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1642430.1522655229334259No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1627870.15091570223244583No Hit
TTCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1616810.14989035766150904No Hit
CCATTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1613180.1495538295609213No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1529990.1418414954871211No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATAGATCGGAA1502440.13928740480635182No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1458530.1352166199862945No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1449180.1343498051817503No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC1443120.1337879979394468No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1429380.13251419736036257No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1336770.12392855895941729No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCTGTAGGCACCATCAATAGATCG1301910.12069677670418619No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAA1279440.11861363994930829No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC1277830.1184643809294884No Hit
TCTAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1214690.11261083154350754No Hit
TCGAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1211360.11230211568263779No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC1194370.11072701583994198No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGA1108310.10274861133950626No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)