FastQCFastQC Report
Mon 3 Jul 2023
SRR4045285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37723150
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length3-36
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG8506392.2549521977883606No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC8421812.232530952478783No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG7797172.066945628877758No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC7051071.869162569933847No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG6930491.8371981130950092No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG5616171.488786063730097No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG4989361.3226254965452249No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG4233911.1223638534957976No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT4170371.105520085146654No Hit
TCGCTTCTGGCGCCAAGCGCCCGG3446180.9135451307751341No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG3410440.9040708424402522No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG2790870.7398295211295982No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTT2435610.6456539286883518No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT2418770.6411898264063314No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA2407870.6383003540266388No Hit
AACGATGCCGACCGGCGATGCGGCGGCG2252790.5971903194722604No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC2193210.5813963043913353No Hit
ACCTCTAGCATCACCAGTATTAGAGGC2134220.5657586919438063No Hit
CCGCGAGGGGGGCCCGGGC2116390.5610321513447313No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG2098120.5561889714936319No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGT2081690.5518335557873614No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC2066020.5476796078800419No Hit
CCCCCCGCGGGGGCGCGCCGGC2018720.5351408882874309No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGG1943540.5152114815438266No Hit
TTCGCTTCTGGCGCCAAGCGCCCGG1792730.475233377912502No Hit
TCGGGTCGCGCCGTCGGGCCCGGGGG1671740.4431602344979144No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCG1654760.4386590197266135No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1594240.4226158207890911No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC1589980.42148654075812864No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC1559850.4134994028865564No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT1553180.4117312578615519No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC1529760.40552286858335No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTA1462670.38773803354173764No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC1414300.3749156684953404No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC1407920.3732243993409882No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1407520.3731183636573298No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCG1400600.3712839463300387No Hit
TAACGATGCCGACCGGCGATGCGGCGGCG1305130.3459758795328598No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1285960.3408941193935289No Hit
AACGATGCCGACCGGCGATGCGGCGGC1263290.3348845470221866No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCCG1257770.3334212545877001No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCC1238790.328389861398107No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1194840.31673919065613554No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1161940.3080177556752286No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC1152090.3054066269651394No Hit
TCGCCCCAGCTGCGGCGGGCGTCGCG1133520.30048392035129623No Hit
TCCCCGCCCCTTGCCTCTCGGC1131020.299821197328431No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCC1096260.2906066964185122No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT1074010.28470846151501134No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1048910.27805472236544404No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGC1038080.2751838062303917No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGC1033060.27385305840047824No Hit
TCCCCCGCGGGGGCGCGCCGGC1007310.2670270112649659No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGG983500.2607152371951971No Hit
TCGATGCCGACCGGCGATGCGGCGGCG975840.2586846538531379No Hit
CCCCCGCCCCTTGCCTCTCGGC950660.25200970756683894No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG947630.2512064872631262No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG936360.2482189318760496No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC922750.2446110677395711No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT910970.2414883168558299No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG901350.23893815866384435No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG887020.23513943029678064No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC886770.2350731579944941No Hit
TCGCGGCGCCTCGCCTCGGCCGGCG854570.2265372854599894No Hit
ACGATGCCGACCGGCGATGCGGCGGCG830490.22015393730375116No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC821670.2178158504790825No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGG810280.21479648438690832No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT804870.21336235176542787No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG798140.21157830138787453No Hit
TCTCGCTTCTGGCGCCAAGCGCCCG785730.20828854430237137No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGC764680.2027084164498458No Hit
TAACGATGCCGACCGGCGATGCGGCGGC761980.2019926755851513No Hit
TCCCCGCCCCTTGCCTCTCGGCG733070.1943289465487373No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC713850.1892339319489491No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC712070.18876207315666904No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGG711830.18869845174647398No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC706600.18731203518263984No Hit
TCTCCCCCGCGGGGGCGCGCCGGC700320.18564727494920227No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT692390.18354511752067365No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC687190.18216665363311388No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC682990.18105327895470022No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC681370.18062383443588353No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGC668860.1773075684294657No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCC656730.17409203632252343No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC651660.1727480340321527No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGC644170.17076251585564833No Hit
ACCCCCGCGGGGAATCCCCC644000.17071745069009348No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC633690.1679843809437971No Hit
CCCCGCCCCTTGCCTCTCGGC633320.16788629793641305No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGT632110.16756553999334625No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC629650.16691342053884684No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCG628960.166730508984536No Hit
GGGTCGCGCCGTCGGGCCCGGGGG616370.16339303584138654No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG614360.16286020653100286No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCC602520.1597215502947129No Hit
TTCCCCCGCGGGGGCGCGCCGGC589650.15630985217300253No Hit
CGCTATTAAAGGTTCGTTTGT579840.15370932703127918No Hit
ACCCCTCCTCCCCGCGC576760.1528928522671092No Hit
TAACGCTGCCGACCGGCGATGCGGCGGCGTT574300.15224073281260977No Hit
TGGGGTCTCCCCCGCGGGGGCGCGCCGGC572830.15185105167516497No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC570670.1512784589834094No Hit
CGGGTCGCGCCGTCGGGCCCGGGGG568880.15080394929903787No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG565880.15000868167159953No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGG562990.1492425738571673No Hit
TTACCATGATCACGAAGGTGGT562830.1492001595837039No Hit
CCCCCGCCCCTTGCCTCTCGGCG558430.14803376706346102No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCC558120.14795158940862574No Hit
TCACCCCCGCGGGGAATCCCCC553560.1467427826149195No Hit
CGCGGCGCCTCGCCTCGGCCGGCG541570.14356436299725767No Hit
AGATACCATGATCACGAAGGTGGT529040.14024279520665692No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCC521290.13818835383577457No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG518290.13739308620833626No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC513900.13622934458018485No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC513520.13612861068070933No Hit
TCGATGCCGACCGGCGATGCGGCGGC499430.13239350372384065No Hit
TCCGCGAGGGGGGCCCGGGC495020.13122446031150634No Hit
TCCCCGCGGGGGCGCGCCGGC486920.12907723771742285No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG486050.12884661010546572No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT480520.12738066677888776No Hit
CCGCTATTAAAGGTTCGTTTGT476770.12638658224458985No Hit
CCGCGCGGGGGGCCCGGGC476110.12621162336655345No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT472030.1251300593932373No Hit
TCCGCTATTAAAGGTTCGTTTGT469200.12437985693135384No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC458930.1216573907534233No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGC453270.12015698582965631No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA451180.11960294938254096No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTC439750.11657297972200095No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCC435800.11552587734587381No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC435720.11550467020914212No Hit
CCCCCGCGGGGGCGCGCCGGC429010.11372592161577175No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC418280.110881514401634No Hit
ACGATGCCGACCGGCGATGCGGCGGC409310.10850366419559343No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGG405340.10745126003528338No Hit
TCGCTATTAAAGGTTCGTTTGT398930.10575203820465681No Hit
TTTTAGCAGTAAACTGAGAGT395090.10473409564153577No Hit
CTCGCTTCTGGCGCCAAGCGCCCG391780.10385665035926214No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG391120.10368169148122573No Hit
CCGCCGGCCGAGGTGGGATCCCGAGGCC388190.10290498009842763No Hit
TCTCCCCCGCGGGGGCGCGCCGGCG387820.10280689709104357No Hit
TCGACTGCATAATTTGTGGTAGTGGGG387180.10263723999719004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)