FastQCFastQC Report
Mon 3 Jul 2023
SRR4045286.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045286.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122186753
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length1-35
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTCTGGCGCCAAGCGCCCGGCCG15501351.268660441447364No Hit
TCGCCCCGAGAGAGGGGCCCGTGCCTT14449831.1826020125111272No Hit
TCCCCGCGGGGGCGCGCCGGC11445230.9366997419106472No Hit
CCCCCGCGGGGGCGCGCCGGC9564150.7827485193914596No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC9485480.7763100145561607No Hit
ATGCGTGCATTTATCAGA9184790.7517009638516214No Hit
AACGATGCCGACCGGCGATGCGGCGGCG9143110.7482897921020948No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC9055300.7411032520031038No Hit
CCCCGCGGGGGCGCGCCGGC8772170.7179313456344977No Hit
CCCGCCCCCG8573630.7016824483420064No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC8543440.6992116403977116No Hit
CCCCCCGCGGGGGCGCGCCGGC8394290.6870049161548634No Hit
ATTGATCATCGACACTTCGAACGCA7325730.5995519006876302No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC7298900.5973560816367712No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC6925160.5667684777579776No Hit
TCGGCCGAGGTGGGATCCCGAGGCC6456390.528403435027036No Hit
TGCGTGCATTTATCAGA6398150.5236369608741465No Hit
CCGGCCGAGGTGGGATCCCGAGGCC6112760.5002800917379316No Hit
TCGCCCCGAGAGAGGGGCCCGTGCCT5392870.4413629028999567No Hit
ACGATGCCGACCGGCGATGCGGCGGCG5272380.4315017684445711No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG5226520.42774849741690085No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG4845510.3965659026883217No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCG4310910.35281320553628265No Hit
CCGCCCCCG4044140.3309802331845253No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG4017200.32877541152108364No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG3986990.3263029667381373No Hit
AGGGCGCCCTGGAATGGGT3958740.32399093214302865No Hit
GCGCCGCGACCGGCTCCGGGACGGCT3945140.32287788186007366No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC3566320.29187452096382327No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG3546440.29024750334432736No Hit
TCGCTTCTGGCGCCAAGCGCCCGG3362640.2752049561379211No Hit
AGGGCGCCCTGGAATGGGTT3214030.2630424265386609No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCC3189840.2610626701897873No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC3154830.2581973841305039No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC3102310.253899045831916No Hit
ACCCGGCCGTCGCCGGCAGTCGAGAGT3016190.24685081859896876No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCG3003870.24584252598970363No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC3000890.24559863703064438No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT2974000.24339790746383122No Hit
ACGGTCCCCCGCGAGGGGGGCCCGGGC2937580.24041722427962384No Hit
ACCCCCCTCGCGG2894850.2369201185008984No Hit
TCGACTGCATAATTTGTGGTAGTGGGG2769100.22662849548019331No Hit
ATGCAGCCCAAAGCGGGTGGT2581840.21130277518709414No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC2560710.2095734551518854No Hit
TAACGATGCCGACCGGCGATGCGGCGGCG2462160.20150793269709033No Hit
ACCCCCGCGGGGAATCCCCCGC2447270.20028930632111974No Hit
ATTTATCAGA2442990.19993902284971923No Hit
ATGAGGGTAATGCGA2431180.19897246962606494No Hit
TCCCCCGCGGGGGCGCGCCGGC2392040.19576917638526656No Hit
CGCTTCTGGCGCCAAGCGCCCGGC2375690.1944310607877435No Hit
CTCGCCCGCCGCGCCGGGGAGGT2308920.18896647495003No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2302680.1884557812907918No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA2298060.1880776715623174No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2282310.18678866112433645No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT2264550.18533514840188936No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC2258740.1848596467736564No Hit
CGGCCGAGGTGGGATCCCGAGGCC2101980.17203010542394886No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2076130.16991449146700868No Hit
ATCAGA2058010.16843151564883632No Hit
TCGCCGAATCCCGGGGCCGAGGGAGC2016560.16503916754380077No Hit
ACCCCCCTCGCGGC1940990.1588543726994693No Hit
ATCCGAGTCACGGC1903340.15577302393820058No Hit
ACGGGGTCGGCGGCGACGTCGGCT1881990.1540256986778264No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCG1870440.15308042435664035No Hit
TGCCCGCCGCGCCGGGGAGGT1868120.15289055107307747No Hit
CGGGTGTTGACGCGATG1813970.1484588104244001No Hit
CCCCGCGGGGGCGCGCCGG1805940.14780161970586125No Hit
TCCCCGCGGGGGCGCGCCGG1799590.14728192343404034No Hit
ACCTCTAGCATCACCAGTATTAGAGGC1747000.14297785620017253No Hit
CCGCCGGC1746410.1429295694599561No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC1738020.14224291564569197No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1699260.1390707223392703No Hit
CCCCCGCGGGGGCGCGCCGG1669400.13662692223272355No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC1624210.13292848529987536No Hit
CGGGGGGC1528790.12511912809402503No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1527270.12499472835651833No Hit
ATTGATCATCGACACTTCGAACG1469370.12025608046070264No Hit
TTGCGTGCATTTATCAGA1456440.1191978642725697No Hit
TCGCCCCGAGAGAGGGGCCCGTGCC1445620.11831233456215993No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG1402490.11478249201040638No Hit
CCGGCCGAGGTGGGATCCCGAGGC1394220.11410565922805069No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG1373470.11240743912721864No Hit
GCGGGGCGGACTGT1363900.11162421183252165No Hit
ATGCAGCCCAAAGCGGGTGG1350630.11053816938731485No Hit
CCGGGGCGGG1299150.10632494669859996No Hit
ACGCG1276080.10443685331420502No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG1250920.10237771029073832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)