FastQCFastQC Report
Mon 3 Jul 2023
SRR4045287.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045287.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111510701
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA29258132.6237957198385833No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA29101122.6097154568152163No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA22036521.976179846631939No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT19716351.768112819952589No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA17187561.5413372748862908No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG16039521.4383839269380971No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC15332691.374997185247719No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA15081151.352439708902915No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT12290661.1021955641728052No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC11929281.0697879121036105No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT11743601.053136595383792No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT11539621.034844180559855No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT11167151.0014420051040662No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA9044730.8111087024733168No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA8782170.787562980166361No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC8767810.7862752113808342No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG8285420.7430156860012924No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC8223340.7374485072961742No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT7494290.6720691317329267No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA7394950.6631605696748333No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA6778600.6078878474631775No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA6117740.5486235800813413No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAG5901020.5291886740089635No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT5813170.5213105063342754No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA5689400.5102111231459302No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT4927600.4418948097187551No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4657690.41768995784539104No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4451880.39923343321104227No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA4105700.36818887902067804No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATA4094750.36720691048296794No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3900290.34976822538314056No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGA3170120.28428841102882135No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA3129890.28068068552452197No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA2946840.2642652206087378No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG2756340.24718165837734263No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2715830.24354882317527538No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG2640590.23680148867506445No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA2592140.2324566141862923No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGC2562210.22977256684988465No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT2453960.2200649783378189No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGAT2329980.20894676287614763No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA2209020.19809937344040193No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC2150320.19283530465833948No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC2125930.19064807062776873No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2044640.18335818730078648No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1995020.17890839014634122No Hit
TCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1946330.17454199305948226No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC1945240.17444424459317137No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1940580.17402634748031942No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGAT1937890.17378511502676322No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGA1921260.17229377833433224No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGA1859680.1667714383752282No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1812260.16251893170324525No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATC1785680.16013530396513245No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT1784360.1600169296756551No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1718420.1541035958513076No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAA1692560.1517845359074552No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTCTGTAGGCACCATCAATAG1655020.14841804285671203No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGA1588920.14249036063363998No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1542730.13834815727685185No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC1534010.13756616954636486No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAA1532820.1374594533308512No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1507850.135220206354904No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1477410.1324904234975619No Hit
TCTTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1456490.13061437036432944No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1400130.12556014691361325Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA1388190.12448939765879509No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCCCTGTAGGC1320490.11841823144847777No Hit
CCGCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1299940.11657535898729576No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1287700.11547770648486912No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1279570.11474862847467886No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCAC1250040.11210045213508252No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAG1208820.10840394591367514No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1196350.10728566758808197No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCTGTAGGCACCATCAATAGATCG1190980.1068040994558899No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA1188170.1065521057032903No Hit
CATTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC1182350.10603018269968548No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1148720.10301432864277303No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1135760.101852108346086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)