FastQCFastQC Report
Sun 2 Jul 2023
SRR4045289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20920577
Total Bases563.6 Mbp
Sequences flagged as poor quality0
Sequence length3-36
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC11918145.696850521857021No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC6457893.0868603671877692No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC4087581.9538562440223326No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG3366401.6091334383368108No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG3219591.5389585096051606No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC2819381.3476588145728485No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT2270591.0853381338382782No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC2246171.0736654156336127No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1943520.9289992336253441No Hit
TCGGGTCGCGCCGTCGGGCCCGGGGG1940910.927751658092413No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC1677300.8017465292663774No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG1645950.7867612829225503No Hit
CCGCGAGGGGGGCCCGGGC1641220.784500351018043No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCC1501390.7176618503399786No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1452770.6944215735541137No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG1399150.6687913053258522No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1366100.6529934618916103No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1278080.6109200525396599No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC1239140.5923067991862748No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC1201100.5741237442925212No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGC1171720.5600801545769986No Hit
TCGCGGCGCCTCGCCTCGGCCGGCG1074070.513403621706992No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGT1061520.5074047431865766No Hit
TTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1001560.4787439658093561No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC992020.4741838621372632No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC939980.449308831204799No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC921340.44039894310754435No Hit
ACCCCTCCTCCCCGCGC913800.43679483601241015No Hit
CCCCCCGCGGGGGCGCGCCGGC886620.42380284253154205No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC869830.4157772512679741No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC866880.4143671563169601No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG864540.4132486403219185No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC844280.4035643949973273No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG805720.38513278099356435No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC801930.3833211674802277No Hit
TCCCCGCCCCTTGCCTCTCGGCG756980.3618351444130819No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCG753750.3602912099412937No Hit
TCGCCCCAGCTGCGGCGGGCGTCGCG736810.3521939189344539No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC733670.35069300430862876No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGG731130.3494788886558913No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCC690600.3301056180238241No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCC685830.32782556618777775No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCC674730.32251978518565716No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC653950.31258698075105673No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCGC647570.3095373516705586No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGC635790.3039065318322721No Hit
GGGTCGCGCCGTCGGGCCCGGGGG629670.30098118230677867No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCC616990.29492016400886073No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGC589760.28190427061356865No Hit
TCCCCGCCCCTTGCCTCTCGGC581650.27802770449400127No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGG571330.2730947621568946No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT567340.2711875489858621No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC561100.2682048396657511No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGC545110.26056164703296664No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC529200.2529566942632605No Hit
CCCCCGCCCCTTGCCTCTCGGCG515540.24642723764263288No Hit
CGCGGCGCCTCGCCTCGGCCGGCG511270.24438618495082617No Hit
ACCCCCGCGGGGAATCCCCC509900.24373132729560948No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG508210.24292351018807942No Hit
TCTCCCCCGCGGGGGCGCGCCGGC507150.24241683200229136No Hit
TCCCCCGCGGGGGCGCGCCGGC498300.2381865471492493No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG481980.23038561508126665No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC478640.2287891007977457No Hit
CGGGTCGCGCCGTCGGGCCCGGGGG473010.22609797043360705No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC471170.22521845358280512No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT453850.21693952322634313No Hit
TTAAGGCCTACTTCACAAAGCGCCTTCC451790.2159548467520757No Hit
ACCCCCCTCGCGG443710.21209262058116274No Hit
TCCGCGAGGGGGGCCCGGGC435230.20803919509485802No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC434680.2077762960361944No Hit
TGGGGTCTCCCCCGCGGGGGCGCGCCGGC431110.2060698421463232No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG426480.20385671007066392No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC424720.20301543308294032No Hit
ACCCCTCCTCCCCGCG412200.19703089451117908No Hit
ACCTCTAGCATCACCAGTATTAGAGGC385680.18435437990070733No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA384240.1836660623652971No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGG381590.1823993669008269No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCG381460.18233722712332456No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCC372510.17805914244143456No Hit
TCACCCCCGCGGGGAATCCCCC367510.17566915099903793No Hit
CCGCGCGGGGGGCCCGGGC362300.17317877991606062No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC361110.17260996195277023No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTT355640.16999531131478832No Hit
CCCCCGCCCCTTGCCTCTCGGC354480.1694408333001523No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGC354440.1694217133686131No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGC352350.1684226969456913No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC337950.161539521591589No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCC337420.16128618249869495No Hit
GTCTAGTGGTTAGGATTCG329240.15737615649893405No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG309980.14816990946282219No Hit
CCCCGCCCCTTGCCTCTCGGCG297110.1420180714900932No Hit
TTCCCCCGCGGGGGCGCGCCGGC293920.14049325694984416No Hit
CCCCGCCCCTTGCCTCTCGGCGCCC293740.14040721725791788No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC283260.1353977951946545No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG278960.1333424025541934No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC275360.13162160871566783No Hit
TCCGTAGTGTAGTGGTTATCACGTTCG273360.13066561213870917No Hit
CCCCGCCCCTTGCCTCTCGGC272480.13024497364484736No Hit
TTCTCCCCCGCGGGGGCGCGCCGGC262190.1253263712563951No Hit
TAGCCCCTCTCCGGCCCCGGCCGGGGGG260240.12439427459386039No Hit
TAAGGCCTACTTCACAAAGCGCCTTCC257490.12307977930054224No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGG244520.11688014149896535No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCCGC243590.11643560309067956No Hit
TCGTTTCCCGGTCAGGG241190.11528840719832918No Hit
GTCTCCCCCGCGGGGGCGCGCCGGC240400.11491078855043052No Hit
TCCTCCTCGGCGCGCGGCGGCGG236320.11296055553343484No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGC233000.11137360121568349No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGG230040.10995872628178467No Hit
TCTCCCCCGCGGGGGCGCGCCGGCG228340.10914612919136982No Hit
TCCCCTCCTCCCCGCGC228030.10899794972194121No Hit
CGGGGGGCGGGGCGGACTGT227970.10896926982463247No Hit
TCCCCGCGAGGGGCCCGGGGCGGGG222410.1063115993406874No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC222130.10617775981991318No Hit
TCGCCGCGGCCCGGGTTCGCTTCCCGGTCAGG212460.10155551637031808No Hit
TTCCCCGCGAGGGGCCCGGGGCGGGG210490.10061385974201381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)