FastQCFastQC Report
Sun 2 Jul 2023
SRR4045290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20446575
Total Bases432 Mbp
Sequences flagged as poor quality0
Sequence length1-35
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGCGCCCTGGAATGGGT2834361.3862272776736446No Hit
CCCCGCGGGGGCGCGCCGGC2739461.3398136362691553No Hit
TCCCCGCGGGGGCGCGCCGGC2344001.1464022703068852No Hit
CCCCCGCGGGGGCGCGCCGGC1852150.9058485345345125No Hit
TCGCTTCTGGCGCCAAGCGCCCGG1820720.8904767668912764No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1650340.8071474073286112No Hit
TCGCCCCGAGAGAGGGGCCCGTGCCTT1624420.7944704675477433No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC1570520.7681090842842873No Hit
CCGGCCGAGGTGGGATCCCGAGGCC1543700.7549919729832502No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1350060.6602866250215501No Hit
ACCTCTAGCATCACCAGTATTAGAGGC1310670.6410217848221523No Hit
AGGGCGCCCTGGAATGGGTT1309790.6405913948913204No Hit
TCGGCCGAGGTGGGATCCCGAGGCC1178110.5761894106959234No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC1173730.5740472426311009No Hit
ACCCCCCTCGCGGC1112230.5439688554195508No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1034230.5058206570049019No Hit
GCGCCGCGACCGGCTCCGGGACGGCT1023580.5006119606829017No Hit
ACCCCCCTCGCGG1022390.5000299561173449No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC918460.44919992712715945No Hit
AACGATGCCGACCGGCGATGCGGCGGC908130.44414773623455267No Hit
CCCCCCGCGGGGGCGCGCCGGC873920.42741632767346116No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG837240.4094768928292391No Hit
ATTGATCATCGACACTTCGAACGCA798180.3903734488539034No Hit
CCCCGCGGGGGCGCGCCGG756220.3698516744246897No Hit
ACGGTCCCCCGCGAGGGGGGCCCGGGC716190.35027382336650514No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG676390.33080846058569713No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC601400.29413239136628017No Hit
CCGGCCGAGGTGGGATCCCGAGGC597620.29228367098157026No Hit
CGGGTGTTGACGCGATG592550.2898040380846181No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC570870.2792007952432131No Hit
TCCCCGCGGGGGCGCGCCGG546570.2673161641986494No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC536940.2626063289328408No Hit
TCGACTGCATAATTTGTGGTAGTGGGG525540.25703082301069985No Hit
ACGATGCCGACCGGCGATGCGGCGGCG513430.2511080706670922No Hit
TCCCCCGCGGGGGCGCGCCGGC507670.24829097293801042No Hit
TCGGCCGAGGTGGGATCCCGAGGC480570.23503691938625415No Hit
CGGCCGAGGTGGGATCCCGAGGCC472380.23103135855271603No Hit
AGCGGTCGGCGTC469540.22964237286684933No Hit
CCGCCGGC463360.2266198617616887No Hit
CCCCCGCGGGGGCGCGCCGG458860.22441900416084354No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC457020.2235190979418313No Hit
CGGGGGGC444370.21733224268612228No Hit
TCTTCCCGGAGTCGGGTTGCTT433150.21184477106801505No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG424650.20768759559975206No Hit
ACCCGGCCGTCGCCGGCAGTCGAGAGT423380.20706646467684686No Hit
ATGCGTGCATTTATCAGA418640.20474822800395664No Hit
ACCCCCGCGGGGAATCCCCCGC406580.19884992963369172No Hit
AGGGCGCCCTGGAATGGG405210.19817989076410109No Hit
ACCATGATCACGAAGGTGGT394090.19274132709267933No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC383940.18777717050410642No Hit
CTCGCCCGCCGCGCCGGGGAGGT378980.18535133634850826No Hit
AAGGGCAGGGCGCCCTGGAATGGGTT361780.1769391695186113No Hit
CGCTTCTGGCGCCAAGCGCCCGG360720.17642074528374557No Hit
TGCCCGCCGCGCCGGGGAGGT349440.17090392889762712No Hit
TCCATCTAAGGCTAAATACC348680.1705322285028177No Hit
GCGGTCGGCGTC343700.16809661275788243No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG341270.16690814965342604No Hit
TCGGCCGAGGTGGGATCCCGAGGCCT341040.16679566137604954No Hit
ATCCGAGTCACGGC339900.16623811078383544No Hit
AACTGTGGTAATTCTAGAGCT338340.16547514681554246No Hit
CCTCTAGCATCACCAGTATTAGAGGC335750.16420843099638938No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG333660.16318625491066352No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC333250.16298573232925317No Hit
TCGCCCCGAGAGAGGGGCCCGTGCCT331520.16213962485159494No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG326770.15981649738403622No Hit
TCGGTTGGCCTCGGATAGCCGGTC326100.15948881414124372No Hit
ACCTCTAGCATCACCAGTATTAGAGG318180.15561530476375626No Hit
AGCGGCCCCCGGCGCGCCGGGC314210.15367365928034402No Hit
TGCGTGCATTTATCAGA313190.15317479822415248No Hit
AGCCGGTC313180.15316990742948391No Hit
AGGGCAGGGCGCCCTGGAATGGGTT305920.14961919050012043No Hit
ACGATGCCGACCGGCGATGCGGCGGC305520.14942355871337865No Hit
TTAGCAGTAAACTGAGAGT301600.14750636720330912No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC301130.14727649985388752No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG300500.1469683797897692No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT298870.1461711802587964No Hit
ATTGATCATCGACACTTCGAACG294750.14415617285535598No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT291100.14237103280133714No Hit
CCCGCGGGGGCGCGCCGGC290020.1418428269771343No Hit
TCGCCGAATCCCGGGGCCGAGGGAGC289350.14151514373434182No Hit
CCCGCCCCCG288460.14107986300884134No Hit
CGGCCGAGGTGGGATCCCGAGGC280180.1370302850232863No Hit
AGGGCAGGGCGCCCTGGAATGGGT276010.13499082364650314No Hit
CGCTTCTGGCGCCAAGCGCCCGGC275900.13493702490514917No Hit
ATGTGGCGT265230.1297185469938119No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC264810.12951313361773303No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC263090.12867191693474334No Hit
AAGGGCAGGGCGCCCTGGAATGGGT259710.12701882833677522No Hit
TGAAGGGCAGGGCGCCCTGGAATGGGTT255180.12480329835192445No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC254590.12451474146648032No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG254270.1243582360370869No Hit
TTCACTGACCCGGTGAGGCGGGG245050.11984892335268865No Hit
TCCCCCGCGGGGGCGCGCCGGCG243730.11920333845644075No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG242840.11876805773094028No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCC233540.11421961868919367No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC228920.11196007155232601No Hit
GTGGGGGGGCCGGGCC223810.10946087547669965No Hit
GCGCCGCGACCGGCTCCGGGA221360.10826263078290618No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT221330.10824795839890056No Hit
AACACGTGCGCGAGTCGGGGGCTCGC209370.10239856797532106No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC207470.10146931698829754No Hit
AATGCAGCCCAAAGCGGGTGGTA205890.10069657143066749No Hit

[OK]Adapter Content

Adapter graph