FastQCFastQC Report
Sun 2 Jul 2023
SRR4045291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37788652
Total Bases972.1 Mbp
Sequences flagged as poor quality0
Sequence length3-35
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC26938617.128756537809287No Hit
TCGACCTGGTATTGCAGTACCTCCAGG15881474.202708792046883No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC4246181.1236653797547476No Hit
ATCCCAGCGGTG3744920.9910170915861195No Hit
TCGACCTGGTATTGCAGTACTTCCAGG3535590.9356221545028915No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC3527490.9334786538561894No Hit
TTCCCGGTCAGG3292130.871195405435473No Hit
ATCCGAGTCACGGC2927180.774618792964618No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC2543440.6730697882528331No Hit
ATCCGAGTCACG2502730.6622967127803341No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC2455930.6499120423771666No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC2383240.6306761087958364No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG2249830.5953718592555246No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC2170280.5743205658672345No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG2124970.5623301937311762No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG2069040.5475294540805531No Hit
ATCTCGCTGGGGCC1925940.5096609426554829No Hit
ATCCCGGACGA1811570.4793952427834684No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG1785380.47246459069246505No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1750570.4632528305058355No Hit
ATCTCGCTGGGG1657280.43856552490943573No Hit
TTCCCGGGCGG1652430.4372820708185092No Hit
ATCCGGGTGCC1610500.4261861471004576No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1601920.4239156241932102No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1518550.4018534453147469No Hit
TCGTTTCCCGGTCAGG1502380.3975743829126268No Hit
TTCCCGGTCAGGG1489000.3940336373999263No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1471940.3895190545563784No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1413200.37397470542214634No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1393960.3688832298119552No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT1345420.3560381037143108No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1254510.3319806168264483No Hit
ACCCCGTCCGTG1239220.3279344285686613No Hit
ATCCCGGACGAG1213360.32109110428178284No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1211880.3206994523117681No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGCA1159120.3067375888401629No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG1137020.30088927226088935No Hit
TCCCCGGCACC1128910.2987431253170926No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT1121270.29672135433674635No Hit
ATCCCAGCGGGG1107000.2929450883826182No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC1104630.2923179159711757No Hit
TCGTTTCCCGGTCAGGG1051520.2782634321012562No Hit
TTCCCGGGCG1050130.2778955968050938No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTA1041200.2755324534995321No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1036500.27428869386502597No Hit
ATCCGGCTCGAAGG991020.26225333467835793No Hit
ATCCCAGCGGGGCC955110.25275048181131204No Hit
ATCCGAGTCACGG941760.24921767518989563No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC936250.24775956549071926No Hit
TCCCCGTACTGG934700.2473493894410417No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG919100.24322116597331916No Hit
ATCCCAGCGGTGCC917410.2427739417643159No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTT892050.2360629323321721No Hit
CCGACCTGGTATTGCAGTACCTCCAGG889410.23536430989917292No Hit
AGAGCACTGG887840.23494884125530596No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA858980.22731162784001926No Hit
ATCCCGGAC847730.2243345436084886No Hit
TCCCCGGCACCT826180.2186317733694232No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG825030.21832744920353336No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG781340.2067657771968156No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCG754150.19957049539634278No Hit
ATCTCGCTGGG735390.19460604204669696No Hit
TTCCCGGCCAATGC728700.19283566929034673No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC704180.18634694881415723No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC651360.1723692075599839No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG618220.16359937898816818No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC615700.16293251212030532No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG612720.16214391558608654No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCC612350.16204600259358284No Hit
ATCCCGGACG608980.161154200472671No Hit
TTCCCGGCCAACG607100.16065669661886856No Hit
CGACCTGGTATTGCAGTACCTCCAGG604480.15996336678005874No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCG595850.15767961238733785No Hit
TTCCCGTGGATCGCCCCAGCTGCGGCGGGCG585980.15506771715487497No Hit
ATCCCAGCGG582930.1542605965409933No Hit
TCGACCTGGTATTGCAGTACCTCCAG573580.15178630875745447No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC571770.15130732898331486No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC570560.15098712703485692No Hit
TTCCGGCTCGAAGG570460.15096066406390998No Hit
GCGACCTGGTATTGCAGTACCTCCAGG552930.14632170525691152No Hit
ATAACTGACGAAGACTACTCCTGTCTGATT536380.14194208356519306No Hit
GCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG533680.1412275833496257No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG526400.13930107906468853No Hit
AACCGGGCGG514370.13611758365977172No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA505450.13375708665130476No Hit
TTCCCGGGC493960.1307164912895014No Hit
TTCCCGGTCAG490230.12972942247318056No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT489900.12964209466905566No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCC488580.12929278345255607No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC483680.12799609787615604No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCA474340.1255244563897119No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG471460.12476232282644006No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG460450.12184874972518206No Hit
TTCCCGGCCCATGC458250.12126656436434939No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC456980.12093048463332325No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG455850.12063145306162285No Hit
TCGACTGCCTAATTTGTGGTAGTGGGGG453000.11987725838963506No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG452110.11964173794820732No Hit
TAGCCGGTCCCC449480.11894576181230282No Hit
ATCCCAGCGGG447500.11842179498755341No Hit
CCGGGGCGGGG440630.11660378888349868No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC437560.11579137567542765No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC436930.11562465895846193No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT435730.11530710330709866No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCG434570.11500013284411416No Hit
TAAGCGCCCGGCCG430870.1140210029190774No Hit
TCGACCTGGTATTGCAGTACCTCCAGGA430790.11399983254231985No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC429940.11377489728927087No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATT419020.1108851408618651No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC418070.11063374263786917No Hit
ATCCCGGACGAGC416150.11012565359568792No Hit
ATCCTGCCGACTACG411820.10897980695368546No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG397430.10517178543442088No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGG395810.10474308530508047No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC394240.10432761666121353No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCG392860.10396242766214578No Hit
TCGACTGCCTAATTTGTGGTAGTGGGGGA391510.10360517755436209No Hit
GTGTAATGGTTAGCACTCTGGACT391200.10352314234442657No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCC385170.10192742519632614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)