FastQCFastQC Report
Sun 2 Jul 2023
SRR4045292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4045292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48570692
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length3-35
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGAGTCACGGC10780872.2196245423062946No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG6472581.3326102086418699No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC5634181.1599958262896481No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC4961061.0214101952675494No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC4909031.010697973996335No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG4864881.0016081302691755No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC4197260.864154869360313No Hit
TTCCCGGGC3965110.8163585563079893No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG3353190.6903731163640823No Hit
TTCCCGGTCAGGG3205110.6598855952062614No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC3076380.6333819579922806No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG3047430.6274215734871555No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG2922140.6016261823076352No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC2780340.572431621933655No Hit
TCGACCTGGTATTGCAGTACCTCCAGG2675800.550908354363162No Hit
ATCCGAGTCACG2666480.5489895017349146No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG2428450.49998258208880364No Hit
ATCCGGCTCGAAGG2375560.4890932993089742No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG2347160.4832461518151728No Hit
TTCCCGGTCAGG2068610.42589675271663824No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC2061480.4244287892789339No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT2055950.42329024260144366No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG1977540.4071467624961983No Hit
TTCCGGCTCGAAGG1973470.40630880861240354No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1890040.38913178342198623No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1815340.37375213842948746No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1799320.3704538531178432No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1744250.3591157400022219No Hit
ATCCGAGTCACGG1689800.34790527588118364No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1653230.3403760440555387No Hit
ATCTCGCTGGGG1646880.33906867128843876No Hit
ATCTCGCTGGGGCC1643600.3383933669299997No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC1643080.33828630648293007No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1632690.33614715639628934No Hit
TTCCCGGGCGG1580150.32532993353275674No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1453020.29915571307899014No Hit
AGAGCACTGG1444650.2974324516521198No Hit
TCGTTTCCCGGTCAGGG1402720.28879967367975734No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT1401780.2886061413331315No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT1392190.2866316996265979No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC1340600.27601006796444244No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTT1313550.2704408658620717No Hit
AGAGCACTGGT1313230.2703749825100289No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGC1292740.26615638912453626No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1278270.2631772262993494No Hit
AACCATCCTCTGC1262700.25997158945151533No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1246690.25667536299462235No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1220870.2513594000266663No Hit
AACCATCCTCTG1158820.23858420629461075No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG1145800.23590357740836798No Hit
ATCCCGGACGAG1133320.2333341266786975No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG1123610.23133497871514783No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA1070320.22036334174526484No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1069140.22012039688460686No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1068170.21992068797372705No Hit
TTCCCGGGCG1020070.21001759661978875No Hit
TTTCTTGCGACCCGGGTTCGTTTCCCGGGC1008460.2076272662534847No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC998130.205500469295352No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTA963480.19836653758196404No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG962170.1980968276095387No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGG960370.19772623375429776No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC941760.19389470506205678No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG937840.19308763399953208No Hit
TCGTTTCCCGGTCAGG926240.1906993624879794No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCC869640.17904624459540333No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG860530.17717062791693394No Hit
ATCCCGGACGA845220.17401852129263468No Hit
GTGTAATGGTTAGCACTCTGG844720.17391557855506773No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT833780.17166319145710338No Hit
ATCCCGGACGAGC833690.17164466176434134No Hit
GTATAGTGGTGAGTATCCCCGC823040.16945198145416582No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC822810.16940462779488505No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCC813420.16747136318337816No Hit
TCGACTGCATAATTTGTGGTAGTGGGG808810.1665222311430111No Hit
TAAGCGCCCGGCCG762180.15692179143751958No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT721160.14847636924752894No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGC717000.14761988567097212No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC714950.14719782044694774No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG703640.14486925572318385No Hit
TTCCCGGGCGGCG670980.13814503610531223No Hit
ATCCGGGTGCC658160.13550558431409623No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC653620.13457086425698855No Hit
GTCTAGGGGTATGATTCTCGCTT649080.13363614419988087No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGG628520.12940313883112886No Hit
GCGCAGCGGAAGCGTGCT627150.1291210757301955No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCG614850.1265886843860491No Hit
ATCCCAGCGGGG612360.12607602955296582No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGC597600.12303715993999015No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC583270.12008682108132206No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG582240.11987475904193416No Hit
TCCCCGTACTGG581660.11975534546635654No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG571540.11767178445800194No Hit
TTCCCGGCCAATGC564100.1161399965230061No Hit
ATCCCAGCGGGGCC563810.11608028973521729No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCC554870.11423967358752064No Hit
ATCCGGCTCGGAGG531700.10946930712866929No Hit
ATCCCAGCGGG526010.10829781877515765No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG523900.10786340042262524No Hit
ATCTCGCTGGG523060.1076904566235128No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGC515060.10604337282244197No Hit
GTGTAATGGTTAGCACTCTGGACT515010.10603307854868528No Hit
TTCCCGGTCAGGGA497890.10250831921439373No Hit
TTCCCGGCCAACG494250.10175889608490651No Hit
GCGCAATGGATAGCGCATTG492280.10135330169889281No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC488240.10052152437935206No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC487150.10029710921145615No Hit
AAGCGCCCGGCCG487070.10028063837344546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)